The Repurposed Drug Disulfiram Inhibits Urease and Aldehyde Dehydrogenase and Prevents In Vitro Growth of the Oomycete Pythium insidiosum
Open accessAbstract
Pythium insidiosum is an oomycete microorganism that causes a life-threatening infectious disease, called pythiosis, in humans and animals. The disease has been increasingly reported worldwide.
Integrity signals
Registry
Cites
Statistics
Claims the paper makes
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
DIS's antifungal effects arise from inactivating key enzymes, including ALDH, urease, and carbamate kinase.
“DIS’s antifungal effects arise from inactivating key enzymes, including ALDH, urease (URE), and carbamate kinase (CK).”
From Off-label and repurposed use of disulfiram beyond alcohol dependence: A systematic review of clinical and preclinical evidence (2026) · cites this paper as [110]
In Pythium insidiosum, DIS binds the oomycete's putative ALDH and URE at low energy, impairing their activities.
“In Pythium insidiosum, DIS binds the oomycete’s putative ALDH and URE at low energy, impairing their activities.110”
From Off-label and repurposed use of disulfiram beyond alcohol dependence: A systematic review of clinical and preclinical evidence (2026) · cites this paper as [110]
Cites
Urease as a Virulence Factor in Experimental Cryptococcosis
Cox, G. M., Mukherjee, J., Cole, G. T., Casadevall, A., & Perfect, J. R. (2000) · Infection and Immunity · doi:10.1128/iai.68.2.443-448.2000 · Cited by 522 · see citing works
Long, T. E. (2017) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00898-17 · Cited by 67 · see citing works
Flagged first, then by citations
Cited by
Sorted by citations
The Promiscuity of Disulfiram in Medicinal Research
Cvek, B. (2023) · ACS Medicinal Chemistry Letters · doi:10.1021/acsmedchemlett.3c00450 · Cited by 21 · see citing works
Antifungal activity of the repurposed drug disulfiram against Cryptococcus neoformans
Peng, M., Zhang, C., Duan, Y.-Y., Liu, H.-B., Peng, X.-Y., Wei, Q., Chen, Q.-Y., Sang, H., & Kong, Q.-T. (2024) · Frontiers in Pharmacology · doi:10.3389/fphar.2023.1268649 · Cited by 9 · see citing works
Long, T. E., Naidu, S. T., Hissom, E. G., Meka, Y., Chavva, H., Brown, K. C., Valentine, M. E., Fan, J., Denvir, J., Primerano, D. A., et al. (2025) · Scientific Reports · doi:10.1038/s41598-025-00078-3 · Cited by 8 · see citing works
Benkő, B.-M., De Brito Parreirinha, N. S., Sebe, I., & Zelkó, R. (2026) · iScience · doi:10.1016/j.isci.2026.115441 · Cited by 2 · see citing works
- Authors
- Krajaejun, T., Lohnoo, T., Yingyong, W., Rujirawat, T., Kumsang, Y., Jongkhajornpong, P., Theerawatanasirikul, S., Kittichotirat, W., Reamtong, O., & Yolanda, H.
- Affiliations
- Mahidol University (TH)
- Kasetsart University (TH)
- King Mongkut's University of Technology Thonburi (TH)
- Atma Jaya Catholic University of Indonesia (ID)
- ORCID
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2019-08
- Volume
- 63
- Issue
- 8
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC6658754
- OpenAlex
- W2945561678
- Cited by
- 23
- References
- 2
- Citations by year
- 2026: 3 · 2025: 3 · 2024: 4 · 2023: 3 · 2022: 3 · 2021: 3 · 2020: 4
- FWCI
- 3.30
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ramathibodi Hospital, Mahidol University (CF_61007)
- Thailand Research Fund (RSA6280092, BRG5980009, MRG6080067)
- Mahidol University (ngor-por 54/2561)
- King Mongkut's University of Technology Thonburi
- Topics
- Health Sciences › Veterinary › Small Animals › Infectious Diseases and Mycology
- Life Sciences › Agricultural and Biological Sciences › Plant Science › Plant Pathogens and Resistance
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Legionella and Acanthamoeba research
- Keywords
- disulfiram, drug repurposing, urease inhibition, aldehyde dehydrogenase inhibition, Pythium insidiosum, pythiosis, oomycete, antifungal activity, in vitro growth inhibition
- MeSH terms
- Aldehyde Dehydrogenase, Animals, Antifungal Agents, Disulfiram, Humans, Oomycetes, Pythium, Urease, Pythiosis, Molecular Docking Simulation, Acetaldehyde Dehydrogenase Inhibitors
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
