The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.
Ribosomal protection as a linezolid resistance mechanism in Mycobacterium abscessus
Open accessPublished version of a preprint
Abstract
Mycobacterium abscessus has emerged as a significant pulmonary pathogen characterized by its resistance to most first-line antimycobacterial drugs. Recent investigations have highlighted the clinical efficacy of including the oxazolidinone antibiotic linezolid in M. abscessus combination therapies, despite moderate resistance frequently being observed in patient isolates. Even with the potential usefulness of linezolid, the mechanisms that drive linezolid resistance in M. abscessus remain poorly understood. In several bacterial pathogens, including Mycobacterium tuberculosis , ATP-binding cassette (ABC) family proteins of the F subtype (ABC-F) have been found to confer antibiotic resistance to ribosome-targeting antibiotics, including linezolid. Here, we identified an M. abscessus ABC-F protein, MAB_2736c, that causes specific resistance to antibiotics that bind the 50S ribosomal subunit, including linezolid, macrolides, and chloramphenicol. These results demonstrate that targeting ABC-F proteins could help combat intrinsic resistance to several ribosome-targeting antibiotics in mycobacteria.
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Highly accurate protein structure prediction with AlphaFold
Jumper, J., Evans, R., Pritzel, A., Green, T., Figurnov, M., Ronneberger, O., Tunyasuvunakool, K., Bates, R., Žídek, A., Potapenko, A., et al. (2021) · Nature · doi:10.1038/s41586-021-03819-2 · Cited by 43,764 · cited 2×
“To validate that MAB_2736c encodes an ABC-F family protein, its predicted tertiary structure was generated using AlphaFold (31), and this predicted structure was aligned with the predicted structure of OcrA, as well as the X-ray crystal structure of PoxtA from Enterococcus faecalis (14) (Fig.”
Miranda-CasoLuengo, A. A., Staunton, P. M., Dinan, A. M., Lohan, A. J., & Loftus, B. J. (2016) · BMC Genomics · doi:10.1186/s12864-016-2868-y · Cited by 81
Akusobi, C., Benghomari, B. S., Zhu, J., Wolf, I. D., Singhvi, S., Dulberger, C. L., Ioerger, T. R., & Rubin, E. J. (2022) · eLife · doi:10.7554/elife.71947 · Cited by 45 · cited 1×
“We utilized an anhydrotetracycline (ATc)-inducible Cas9/CRISPRi system in the M. abscessus subspecies abscessus reference strain (ATC19977) to determine whether depletion of this protein is sufficient to modulate linezolid susceptibility (32, 33).”
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Research works printed but not linked: 41
- 10.1513/AnnalsATS.201804-236OC · DOI
- 10.1128/AAC.01206-17 · DOI
- 10.3389/fmicb.2019.01977 · DOI
- 10.1093/jac/dkr578 · DOI
- 10.1016/j.drudis.2018.04.001 · DOI
- 10.1128/AAC.01842-18 · DOI
- 10.1016/j.diagmicrobio.2018.10.022 · DOI
- World Health Organization. 2022. WHO consolidated guidelines on tuberculosis. Module 4: treatment - drug-resistant tuberculosis treatment, 2022 update
- 10.1056/NEJMoa2119430 · DOI
- 10.1016/j.micpath.2022.105734 · DOI
- 10.1146/annurev.biochem.73.011303.073626 · DOI
- 10.1021/acsinfecdis.7b00251 · DOI
- 10.1128/mBio.01975-15 · DOI
- 10.1038/s41467-022-29274-9 · DOI
- 10.1093/nar/gky050 · DOI
- 10.1038/s41579-020-0386-z · DOI
- 10.1002/pro.3589 · DOI
- 10.1073/pnas.1808535115 · DOI
- 10.1038/s41467-021-23753-1 · DOI
- 10.1073/pnas.1803313115 · DOI
- 10.1128/AAC.01184-21 · DOI
- 10.1093/jac/dkv116 · DOI
- 10.1016/j.cmi.2015.08.007 · DOI
- 10.1093/jac/dkx292 · DOI
- 10.1016/j.jgar.2017.05.002 · DOI
- 10.1128/CMR.00188-20 · DOI
- 10.1093/jac/dky088 · DOI
- 10.1038/s41564-022-01130-y · DOI
- 10.1093/nar/gkab1112 · DOI · Paper page
- 10.1016/j.tube.2010.09.006 · DOI
- 10.1038/nmicrobiol.2016.274 · DOI · Paper page
- 10.1016/j.jmb.2018.12.013 · DOI
- 10.1128/spectrum.02199-23 · DOI
- 10.1128/mbio.03376-24 · DOI
- 10.1073/pnas.2302033120 · DOI
- 10.2217/fmb-2018-0207 · DOI
- 10.1038/90802 · DOI
- 10.1371/journal.ppat.1005010 · DOI
- 10.1093/nar/gkl521 · DOI
- 10.1093/molbev/mst010 · DOI · Paper page
- 10.1093/bioinformatics/btp033 · DOI
Metadata
- Authors
- Funck, T., McGowen, K., Sullivan, M. R., Zinga, S., Wolf, I. D., Nurjadi, D., Denkinger, C. M., & Rubin, E. J.
- Affiliations
- Harvard University (US)
- University Hospital Schleswig-Holstein (DE)
- University Hospital Heidelberg (DE)
- Corresponding author
- Rubin, Eric J.
- ORCID
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2026-04-01
- Volume
- 70
- Issue
- 4
- Pages
- e0160525-e0160525
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC13041422
- OpenAlex
- W7133187841
- Cited by
- 1
- References
- 3
- Citations by year
- 2026: 1
- FWCI
- 4.63
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion, cc-by)
- bioRxiv (Cold Spring Harbor Laboratory) (acceptedVersion, cc-by)
- PubMed (publishedVersion)
- PubMed Central (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (submittedVersion, cc-by)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion)
- Funders
- National Institutes of Health (R01AI179642)
- National Institute of General Medical Sciences (T32GM145407, T32GM007753, T32GM144273)
- Damon Runyon Cancer Research Foundation (DRG-2415-20)
- Boehringer Ingelheim Fonds (MD Fellowship)
- Topics
- Health Sciences › Medicine › Epidemiology › Mycobacterium research and diagnosis
- Health Sciences › Medicine › Infectious Diseases › Tuberculosis Research and Epidemiology
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Keywords
- Mycobacterium abscessus, linezolid resistance, ribosome-targeting antibiotics, 50S ribosomal subunit, macrolides, chloramphenicol, intrinsic antibiotic resistance, oxazolidinones
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