Role of Klebsiella pneumoniae OmpK35 Porin in Antimicrobial Resistance
Open accessAbstract
OmpK35 from Klebsiella pneumoniae is the homologue of Escherichia coli OmpF porin. Expression of OmpK35 in K. pneumoniae strain CSUB10R (lacking both OmpK35 and OmpK36) decreased the MICs of cephalosporins and meropenem ≥128-fold and decreased the MICs of imipenem, ciprofloxacin, and chloramphenicol ≥8-fold. MIC reductions by OmpK35 were 4 times (cefepime), 8 times (cefotetan, cefotaxime, and cefpirome), or 128 times (ceftazidime) higher than those caused by OmpK36, but the MICs were similar or 1 dilution lower for other evaluated agents.
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K. pneumoniae post-therapy isolates exhibit a shift in porin expression from OmpK35 to OmpK36, the latter having a smaller channel size.
“The post-therapy isolates were found to exhibit a shift in porin expression from OmpK35 to OmpK36 (the latter possessing a smaller channel size).”
From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [42]
This porin shift correlated with a 4-8 fold decrease in susceptibility for a wide range of beta-lactam antimicrobials.
“This alteration in the type of porin expressed correlated with a 4 – 8 fold decrease in susceptibility for a wide range of β-lactam antimicrobials (41,42).”
From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [42]
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Mechanisms of Antibiotic Resistance
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Mechanisms of antibiotic resistance
Munita, J. M., & Arias, C. A. (2016) · Virulence Mechanisms of Bacterial Pathogens · doi:10.1128/9781555819286.ch17 · Cited by 421 · see citing works
- Authors
- Doménech-Sánchez, A., Martínez-Martínez, L., Hernández-Allés, S., del Carmen Conejo, M., Pascual, A., Tomás, J. M., Albertí, S., & Benedí, V. J.
- Affiliations
- Laboratory of Microbiology, Department of Biology, University of the Balearic Island
- Department of Microbiology, School of Medicine, University of Seville
- Department of Microbiology, University of Barcelona, Barcelona, Spain
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2003-10
- Volume
- 47
- Issue
- 10
- Pages
- 3332-3335
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC201126
- OpenAlex
- W4297572977
- Cited by
- 151
- References
- 19
- Citations by year
- 2026: 6 · 2025: 6 · 2024: 9 · 2023: 11 · 2022: 10 · 2021: 9 · 2020: 9 · 2019: 7 · 2018: 4 · 2017: 7 · 2016: 7 · 2015: 10 · 2014: 2 · 2013: 7 · 2012: 8
- FWCI
- 2.04
- Type
- journal article
- Language
- en
- Open access
- green
- europepmc.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- DIGITAL.CSIC (Spanish National Research Council (CSIC)) (submittedVersion)
- Funders
- Ministerio de Ciencia y Tecnología
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Enterobacteriaceae and Cronobacter Research
- Keywords
- Klebsiella pneumoniae, outer membrane porins, antimicrobial resistance, minimum inhibitory concentration, cephalosporins, meropenem, imipenem, ciprofloxacin, chloramphenicol, porin expression
- MeSH terms
- Amino Acid Sequence, Anti-Infective Agents, Bacterial Proteins, Klebsiella pneumoniae, Microbial Sensitivity Tests, Molecular Sequence Data, Sequence Alignment, Sequence Homology, Amino Acid, Protein Structure, Secondary, Porins, Drug Resistance, Bacterial
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