Cited by 942Antibiotic Resistance in BacteriaBacterial Genetics and BiotechnologyDrug Transport and Resistance Mechanisms

The porin and the permeating antibiotic: a selective diffusion barrier in Gram-negative bacteria

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Pagès, Jean-Marie, James, Chloë E., Winterhalter, Mathias·2008·Nature Reviews Microbiology

Abstract

Gram-negative bacteria are responsible for a large proportion of antibiotic-resistant bacterial diseases. These bacteria have a complex cell envelope that comprises an outer membrane and an inner membrane that delimit the periplasm. The outer membrane contains various protein channels, called porins, which are involved in the influx of various compounds, including several classes of antibiotics. Bacterial adaptation to reduce influx through porins is an increasing problem worldwide that contributes, together with efflux systems, to the emergence and dissemination of antibiotic resistance. An exciting challenge is to decipher the genetic and molecular basis of membrane impermeability as a bacterial resistance mechanism. This Review outlines the bacterial response towards antibiotic stress on altered membrane permeability and discusses recent advances in molecular approaches that are improving our knowledge of the physico-chemical parameters that govern the translocation of antibiotics through porin channels.

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  • Hydrophilic molecules such as β-lactams, tetracyclines and some fluoroquinolones are particularly affected by changes in permeability of the outer membrane since they often use water-filled diffusion channels known as porins to cross this barrier.

    “Hydrophilic molecules such as β-lactams, tetracyclines and some fluoroquinolones are particularly affected by changes in permeability of the outer membrane since they often use water-filled diffusion channels known as porins to cross this barrier (37).”

    From Mechanisms of Antibiotic Resistance (2016) · cites this paper as [37]

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Authors
Pagès, J.-M., James, C. E., & Winterhalter, M.
Affiliations
  • Aix-Marseille Université (FR)
  • Constructor University (DE)
Corresponding author
Pagès, Jean-Marie
Venue
Nature Reviews Microbiology
Published
2008-11-10
Volume
6
Issue
12
Pages
893-903
ISSN
  • 1740-1526 (ISSN-L)
  • 1740-1534
OpenAlex
W1996764065
Cited by
939
References
96
Citations by year
2026: 48 · 2025: 59 · 2024: 57 · 2023: 50 · 2022: 75 · 2021: 64 · 2020: 84 · 2019: 67 · 2018: 57 · 2017: 60 · 2016: 60 · 2015: 53 · 2014: 42 · 2013: 32 · 2012: 47
FWCI
1.55
Type
journal article
Language
en
Open access
Locations
  • Nature Reviews Microbiology (publishedVersion)
  • PubMed (publishedVersion)
  • University of Salford Institutional Repository (University of Salford) (submittedVersion, cc-by)
Funders
European Commission
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Medicine › Antibiotic Resistance in Bacteria
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Health Sciences › Medicine › Oncology › Drug Transport and Resistance Mechanisms
Keywords
porin, antibiotic permeation, Gram-negative bacteria, outer membrane, antibiotic resistance, membrane permeability, efflux systems, periplasm, antibiotic translocation, bacterial cell envelope
MeSH terms
Animals, Anti-Bacterial Agents, Bacterial Proteins, Cell Membrane Permeability, Diffusion, Gram-Negative Bacteria, Humans, Models, Biological, Models, Molecular, Mutation, Porins, Drug Resistance, Bacterial

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