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Use of Genetically Engineered Phage To Deliver Antimicrobial Agents to Bacteria: an Alternative Therapy for Treatment of Bacterial Infections
Open accessAbstract
The emergence and increasing prevalence of multidrug-resistant bacterial pathogens emphasizes the need for new and innovative antimicrobial strategies. Lytic phages, which kill their host following amplification and release of progeny phage into the environment, may offer an alternative strategy for combating bacterial infections. In this study, however, we describe the use of a nonlytic phage to specifically target and deliver DNA encoding bactericidal proteins to bacteria. To test the concept of using phage as a lethal-agent delivery vehicle, we used the M13 phagemid system and the addiction toxins Gef and ChpBK. Phage delivery of lethal-agent phagemids reduced target bacterial numbers by several orders of magnitude in vitro and in a bacteremic mouse model of infection. Given the powerful genetic engineering tools available and the present knowledge in phage biology, this technology may have potential use in antimicrobial therapies and DNA vaccine development.
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Citations
Cited by
Sequence-specific antimicrobials using efficiently delivered RNA-guided nucleases
Citorik, R. J., Mimee, M., & Lu, T. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.3011 · Cited by 808
Bacteriophage therapy: a potential solution for the antibiotic resistance crisis
Golkar, Z., Bagasra, O., & Pace, D. G. (2014) · The Journal of Infection in Developing Countries · doi:10.3855/jidc.3573 · Cited by 593
More Is Better: Selecting for Broad Host Range Bacteriophages
Ross, A., Ward, S., & Hyman, P. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.01352 · Cited by 367
Revisiting phage therapy: new applications for old resources
Nobrega, F. L., Costa, A. R., Kluskens, L. D., & Azeredo, J. (2015) · Trends in Microbiology · doi:10.1016/j.tim.2015.01.006 · Cited by 310
Experimental Phage Therapy against Staphylococcus aureus in Mice
Capparelli, R., Parlato, M., Borriello, G., Salvatore, P., & Iannelli, D. (2007) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01513-06 · Cited by 300
Metadata
- Authors
- Westwater, C., Kasman, L. M., Schofield, D. A., Werner, P. A., Dolan, J. W., Schmidt, M. G., & Norris, J. S.
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2003-04
- Volume
- 47
- Issue
- 4
- Pages
- 1301-1307
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- OpenAlex
- W2138608758
- Cited by
- 214
- References
- 0
- FWCI
- 6.96
- Type
- journal article
- Language
- en
- Open access
- green
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- Westwater, C., Kasman, L. M., Schofield, D. A., Werner, P. A., Dolan, J. W., Schmidt, M. G., & Norris, J. S. (2003). Use of Genetically Engineered Phage To Deliver Antimicrobial Agents to Bacteria: an Alternative Therapy for Treatment of Bacterial Infections. Antimicrobial Agents and Chemotherapy. https://doi.org/10.1128/aac.47.4.1301-1307.2003
Data sources
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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