Atomic Force Microscopy Study of the Effect of Antimicrobial Peptides on the Cell Envelope of Escherichia coli
Open accessAbstract
The influences of the antibacterial magainin 2 and PGLa from the African clawed frog (Xenopus laevis) and the hemolytic bee venom melittin on Escherichia coli as the target cell were studied by atomic force microscopy (AFM). Nanometer-scale images of the effects of the peptides on this gram-negative bacterium's cell envelope were obtained in situ without the use of fixing agents. These high-resolution AFM images of the surviving and intact target cells before and after peptide treatment showed distinct changes in cell envelope morphology as a consequence of peptide action. Although all three peptides are lytic to E. coli, it is clear from this AFM study that each peptide causes distinct morphological changes in the outer membrane and in some cases the inner membrane, probably as a consequence of different mechanisms of action.
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Carvacrol-induced morphological changes of some strains were comparable to those described after treatment with other antimicrobial agents, such as antimicrobial peptides.
“The morphological changes of some strains after carvacrol contact were comparable to those described after treatment with other antimicrobial agents, such as antimicrobial peptides [119].”
From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [119]
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- Authors
- Meincken, M., Holroyd, D. L., & Rautenbach, M.
- Affiliations
- Stellenbosch University (ZA)
- Venue
- Antimicrobial Agents and Chemotherapy
- Publisher
- American Society for Microbiology
- Published
- 2005-10
- Volume
- 49
- Issue
- 10
- Pages
- 4085-4092
- ISSN
- 0066-4804 (ISSN-L)
- 1098-6596
- PMC
- PMC1251503
- OpenAlex
- W2089546856
- Cited by
- 192
- References
- 35
- Citations by year
- 2026: 6 · 2025: 8 · 2024: 5 · 2023: 7 · 2022: 6 · 2021: 12 · 2020: 9 · 2019: 10 · 2018: 14 · 2017: 11 · 2016: 17 · 2015: 7 · 2014: 9 · 2013: 13 · 2012: 10
- FWCI
- 5.58
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Antimicrobial Agents and Chemotherapy (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
- Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- antimicrobial peptides, Escherichia coli, cell envelope, magainin 2, PGLa, melittin, outer membrane, inner membrane, membrane morphology, peptide-membrane interactions, Gram-negative bacteria, in situ imaging
- MeSH terms
- Animals, Anti-Bacterial Agents, Escherichia coli, Melitten, Xenopus laevis, Microscopy, Atomic Force, Antimicrobial Cationic Peptides, Xenopus Proteins, Magainins
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