Cited by 192Antimicrobial Peptides and ActivitiesAntimicrobial agents and applicationsBacterial Genetics and Biotechnology

Atomic Force Microscopy Study of the Effect of Antimicrobial Peptides on the Cell Envelope of Escherichia coli

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Meincken, M., Holroyd, D. L., Rautenbach, M.·2005·Antimicrobial Agents and Chemotherapy

Abstract

The influences of the antibacterial magainin 2 and PGLa from the African clawed frog (Xenopus laevis) and the hemolytic bee venom melittin on Escherichia coli as the target cell were studied by atomic force microscopy (AFM). Nanometer-scale images of the effects of the peptides on this gram-negative bacterium's cell envelope were obtained in situ without the use of fixing agents. These high-resolution AFM images of the surviving and intact target cells before and after peptide treatment showed distinct changes in cell envelope morphology as a consequence of peptide action. Although all three peptides are lytic to E. coli, it is clear from this AFM study that each peptide causes distinct morphological changes in the outer membrane and in some cases the inner membrane, probably as a consequence of different mechanisms of action.

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Crossref · 10.1128/aac.49.10.4085-4092.2005

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33 of 33 references verified0 citation errors · 0 not checkable

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  • Carvacrol-induced morphological changes of some strains were comparable to those described after treatment with other antimicrobial agents, such as antimicrobial peptides.

    “The morphological changes of some strains after carvacrol contact were comparable to those described after treatment with other antimicrobial agents, such as antimicrobial peptides [119].”

    From Effect of essential oils on pathogenic bacteria (2013) · cites this paper as [119]

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Authors
Meincken, M., Holroyd, D. L., & Rautenbach, M.
Affiliations
Stellenbosch University (ZA)
Venue
Antimicrobial Agents and Chemotherapy
Published
2005-10
Volume
49
Issue
10
Pages
4085-4092
ISSN
  • 0066-4804 (ISSN-L)
  • 1098-6596
OpenAlex
W2089546856
Cited by
192
References
35
Citations by year
2026: 6 · 2025: 8 · 2024: 5 · 2023: 7 · 2022: 6 · 2021: 12 · 2020: 9 · 2019: 10 · 2018: 14 · 2017: 11 · 2016: 17 · 2015: 7 · 2014: 9 · 2013: 13 · 2012: 10
FWCI
5.58
Type
journal article
Language
en
Open access
Locations
  • Antimicrobial Agents and Chemotherapy (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Microbiology › Antimicrobial Peptides and Activities
  • Physical Sciences › Chemistry › Organic Chemistry › Antimicrobial agents and applications
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
antimicrobial peptides, Escherichia coli, cell envelope, magainin 2, PGLa, melittin, outer membrane, inner membrane, membrane morphology, peptide-membrane interactions, Gram-negative bacteria, in situ imaging
MeSH terms
Animals, Anti-Bacterial Agents, Escherichia coli, Melitten, Xenopus laevis, Microscopy, Atomic Force, Antimicrobial Cationic Peptides, Xenopus Proteins, Magainins

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