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Cited by 97Probiotics and Fermented FoodsBacterial Genetics and BiotechnologyGenomics and Phylogenetic Studies

Comparative High-Density Microarray Analysis of Gene Expression during Growth of Lactobacillus helveticus in Milk versus Rich Culture Medium

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Smeianov, Vladimir V., Wechter, Patrick, Broadbent, Jeffery R., Hughes, Joanne E., Rodríguez, Beatriz T., Christensen, Tove K., Ardö, Ylva, Steele, James L.·2007·Applied and Environmental Microbiology
DOI 10.1128/aem.00005-07PMC PMC1855617
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Abstract

Lactobacillus helveticus CNRZ32 is used by the dairy industry to modulate cheese flavor. The compilation of a draft genome sequence for this strain allowed us to identify and completely sequence 168 genes potentially important for the growth of this organism in milk or for cheese flavor development. The primary aim of this study was to investigate the expression of these genes during growth in milk and MRS medium by using microarrays. Oligonucleotide probes against each of the completely sequenced genes were compiled on maskless photolithography-based DNA microarrays. Additionally, the entire draft genome sequence was used to produce tiled microarrays in which noninterrupted sequence contigs were covered by consecutive 24-mer probes and associated mismatch probe sets. Total RNA isolated from cells grown in skim milk or in MRS to mid-log phase was used as a template to synthesize cDNA, followed by Cy3 labeling and hybridization. An analysis of data from annotated gene probes identified 42 genes that were upregulated during the growth of CNRZ32 in milk ( P < 0.05), and 25 of these genes showed upregulation after applying Bonferroni's adjustment. The tiled microarrays identified numerous additional genes that were upregulated in milk versus MRS. Collectively, array data showed the growth of CNRZ32 in milk-induced genes encoding cell-envelope proteinases, oligopeptide transporters, and endopeptidases as well as enzymes for lactose and cysteine pathways, de novo synthesis, and/or salvage pathways for purines and pyrimidines and other functions. Genes for a hypothetical phosphoserine utilization pathway were also differentially expressed. Preliminary experiments indicate that cheese-derived, phosphoserine-containing peptides increase growth rates of CNRZ32 in a chemically defined medium. These results suggest that phosphoserine is used as an energy source during the growth of L. helveticus CNRZ32.

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Crossref · 10.1128/aem.00005-07

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  • L. helveticus CNRZ 32 genes encoding CEPs, endopeptidases and oligopeptide transporters were upregulated when the strain was grown in milk compared with the levels of expression in MRS medium.

    “Smeianov et al. (2007) showed that L. helveticus CNRZ 32 genes encoding CEPs, endopeptidases and oligopeptide transporters were upregulated when the strain was grown in milk compared with the levels of expression in MRS medium.”

    From Lactobacillus helveticus: the proteolytic system (2013) · cites this paper as Smeianov et al., 2007

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Authors
Smeianov, V. V., Wechter, P., Broadbent, J. R., Hughes, J. E., Rodríguez, B. T., Christensen, T. K., Ardö, Y., & Steele, J. L.
Affiliations
  • University of Wisconsin–Madison (US)
  • Utah State University (US)
  • Department of Nutrition and Food Sciences, Utah State University, Logan, Utah
  • Department of Food Science, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark
ORCID
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
2007-04-15
Volume
73
Issue
8
Pages
2661-2672
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.00005-07
PMC
PMC1855617
OpenAlex
W2147318137
Cited by
97
References
53
Citations by year
2026: 2 · 2025: 3 · 2024: 1 · 2023: 3 · 2022: 1 · 2021: 4 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 7 · 2016: 4 · 2015: 5 · 2014: 6 · 2013: 8 · 2012: 8
FWCI
15.74
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Digital Commons - USU (Utah State University) (submittedVersion)
  • Research at the University of Copenhagen (University of Copenhagen) (submittedVersion)
Funders
Dairy Management
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Genomics and Phylogenetic Studies
Keywords
gene expression, milk, MRS medium, cheese flavor, cheese starter cultures, cell envelope proteinase, oligopeptide transporter, endopeptidase, purine and pyrimidine metabolism, lactose metabolism, cysteine metabolism, upregulated genes, oligonucleotide microarrays
MeSH terms
Adaptation, Physiological, Animals, Carbocyanines, Culture Media, DNA, Bacterial, Enzymes, Milk, Molecular Sequence Data, Nucleic Acid Hybridization, RNA, Bacterial, Staining and Labeling, Gene Expression Regulation, Bacterial, DNA, Complementary, Oligonucleotide Array Sequence Analysis, Gene Expression Profiling, Lactobacillus helveticus, Metabolic Networks and Pathways

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