Cited by 118Probiotics and Fermented FoodsReproductive tract infections researchBacterial Genetics and Biotechnology

Lactobacillaceae and Cell Adhesion: Genomic and Functional Screening

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Turpin, Williams, Humblot, Christèle, Noordine, Marie-Louise, Thomas, Muriel, Guyot, Jean-Pierre·2012·PLoS ONE

Abstract

The analysis of collections of lactic acid bacteria (LAB) from traditional fermented plant foods in tropical countries may enable the detection of LAB with interesting properties. Binding capacity is often the main criterion used to investigate the probiotic characteristics of bacteria. In this study, we focused on a collection of 163 Lactobacillaceace comprising 156 bacteria isolated from traditional amylaceous fermented foods and seven strains taken from a collection and used as controls. The collection had a series of analyses to assess binding potential for the selection of new probiotic candidates. The presence/absence of 14 genes involved in binding to the gastrointestinal tract was assessed. This enabled the detection of all the housekeeping genes (ef-Tu, eno, gap, groEl and srtA) in the entire collection, of some of the other genes (apf, cnb, fpbA, mapA, mub) in 86% to 100% of LAB, and of the other genes (cbsA, gtf, msa, slpA) in 0% to 8% of LAB. Most of the bacteria isolated from traditional fermented foods exhibited a genetic profile favorable for their binding to the gastrointestinal tract. We selected 30 strains with different genetic profiles to test their binding ability to non-mucus (HT29) and mucus secreting (HT29-MTX) cell lines as well as their ability to degrade mucus. Assays on both lines revealed high variability in binding properties among the LAB, depending on the cell model used. Finally, we investigated if their binding ability was linked to tighter cross-talk between bacteria and eukaryotic cells by measuring the expression of bacterial genes and of the eukaryotic MUC2 gene. Results showed that wild LAB from tropical amylaceous fermented food had a much higher binding capacity than the two LAB currently known to be probiotics. However their adhesion was not linked to any particular genetic equipment.

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  1. [7]Ljungh Å Wadström T 2009 Lactobacillus Molecular Biology: From Genomics to Probiotics Caister Academic Press
  2. [19]Christie J McNab R Jenkinson HF 2002 Expression of fibronectin-binding protein FbpA modulates adhesion in Streptococcus gordonii. Microbiology 148 1615 1625 12055283
  3. [23]Roos S Jonsson H 2002 A high-molecular-mass cell-surface protein from Lactobacillus reuteri 1063 adheres to mucus components. Microbiology-Sgm 148 433 442 11832507

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Authors
Turpin, W., Humblot, C., Noordine, M.-L., Thomas, M., & Guyot, J.-P.
Affiliations
  • Institut de Recherche pour le Développement (FR)
  • Microbiologie de l’alimentation au service de la santé (FR)
Corresponding author
Humblot, Christèle
Venue
PLoS ONE
Published
2012-05-31
Volume
7
Issue
5
Pages
e38034
ISSN
1932-6203 (ISSN-L)
OpenAlex
W2166637116
Cited by
118
References
75
Citations by year
2026: 3 · 2025: 6 · 2024: 4 · 2023: 7 · 2022: 14 · 2021: 9 · 2020: 6 · 2019: 11 · 2018: 10 · 2017: 6 · 2016: 5 · 2015: 14 · 2014: 15 · 2013: 6
FWCI
17.21
Type
journal article
Language
en
Open access
Locations
  • PLoS ONE (publishedVersion, cc-by)
  • Europe PMC (PubMed Central) (publishedVersion, cc-by)
  • PubMed Central (publishedVersion, cc-by)
  • PubMed (publishedVersion)
  • HAL (submittedVersion, other-oa)
  • DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Keywords
Lactobacillaceae, lactic acid bacteria, cell adhesion, probiotics, mucus degradation, traditional fermented foods, host-bacteria interactions
MeSH terms
Bacterial Adhesion, Humans, Intestinal Mucosa, Lactobacillaceae, Mucins, Gene Expression Regulation, Bacterial, Genome, Bacterial, HT29 Cells, Mucin-2

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