Lactobacillaceae and Cell Adhesion: Genomic and Functional Screening
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The analysis of collections of lactic acid bacteria (LAB) from traditional fermented plant foods in tropical countries may enable the detection of LAB with interesting properties. Binding capacity is often the main criterion used to investigate the probiotic characteristics of bacteria. In this study, we focused on a collection of 163 Lactobacillaceace comprising 156 bacteria isolated from traditional amylaceous fermented foods and seven strains taken from a collection and used as controls. The collection had a series of analyses to assess binding potential for the selection of new probiotic candidates. The presence/absence of 14 genes involved in binding to the gastrointestinal tract was assessed. This enabled the detection of all the housekeeping genes (ef-Tu, eno, gap, groEl and srtA) in the entire collection, of some of the other genes (apf, cnb, fpbA, mapA, mub) in 86% to 100% of LAB, and of the other genes (cbsA, gtf, msa, slpA) in 0% to 8% of LAB. Most of the bacteria isolated from traditional fermented foods exhibited a genetic profile favorable for their binding to the gastrointestinal tract. We selected 30 strains with different genetic profiles to test their binding ability to non-mucus (HT29) and mucus secreting (HT29-MTX) cell lines as well as their ability to degrade mucus. Assays on both lines revealed high variability in binding properties among the LAB, depending on the cell model used. Finally, we investigated if their binding ability was linked to tighter cross-talk between bacteria and eukaryotic cells by measuring the expression of bacterial genes and of the eukaryotic MUC2 gene. Results showed that wild LAB from tropical amylaceous fermented food had a much higher binding capacity than the two LAB currently known to be probiotics. However their adhesion was not linked to any particular genetic equipment.
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According to Turpin et al., the same housekeeping genes were present in all their isolates, so screening them was not essential to evaluate bacteria's binding capacity.
“According to Turpin et. al. the same housekeeping genes were present in all their isolates, therefore screening them was not essential to evaluate the bacteria's binding capacity [32].”
From EVALUATION OF ADHESIVE AND AGGREGATION PROPERTIES OF LACTIC ACID BACTERIA ISOLATED FROM DIFFERENT BIOTOPES (2025) · cites this paper as [32]
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- Authors
- Turpin, W., Humblot, C., Noordine, M.-L., Thomas, M., & Guyot, J.-P.
- Affiliations
- Institut de Recherche pour le Développement (FR)
- Microbiologie de l’alimentation au service de la santé (FR)
- Corresponding author
- Humblot, Christèle
- Venue
- PLoS ONE
- Publisher
- Public Library of Science (PLoS)
- Published
- 2012-05-31
- Volume
- 7
- Issue
- 5
- Pages
- e38034
- ISSN
- 1932-6203 (ISSN-L)
- PMC
- PMC3364998
- OpenAlex
- W2166637116
- Cited by
- 118
- References
- 75
- Citations by year
- 2026: 3 · 2025: 6 · 2024: 4 · 2023: 7 · 2022: 14 · 2021: 9 · 2020: 6 · 2019: 11 · 2018: 10 · 2017: 6 · 2016: 5 · 2015: 14 · 2014: 15 · 2013: 6
- FWCI
- 17.21
- Type
- journal article
- Language
- en
- Open access
- gold
- doi.org
- Locations
- PLoS ONE (publishedVersion, cc-by)
- Europe PMC (PubMed Central) (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HAL (submittedVersion, other-oa)
- DOAJ (DOAJ: Directory of Open Access Journals) (submittedVersion, cc-by-sa)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lactobacillaceae, lactic acid bacteria, cell adhesion, probiotics, mucus degradation, traditional fermented foods, host-bacteria interactions
- MeSH terms
- Bacterial Adhesion, Humans, Intestinal Mucosa, Lactobacillaceae, Mucins, Gene Expression Regulation, Bacterial, Genome, Bacterial, HT29 Cells, Mucin-2
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