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Cited by 103Bacterial Genetics and BiotechnologyProbiotics and Fermented FoodsMicrobial metabolism and enzyme function

The DOI matches its Crossref record; 43 of the 44 references it cites were found in a registry or an index.

Lactobacillus plantarum WCFS1 Electron Transport Chains

Open access
Brooijmans, R. J. W., de Vos, W. M., Hugenholtz, J.·2009·Applied and Environmental Microbiology
DOI 10.1128/aem.00147-09PMC PMC2687314
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Abstract

Lactobacillus plantarum WCFS1 requires both heme and menaquinone to induce respiration-like behavior under aerobic conditions. The addition of these compounds enhanced both biomass production, without progressive acidification, and the oxygen consumption rate. When both heme and menaquinone were present, L. plantarum WCFS1 was also able to reduce nitrate. The ability to reduce nitrate was severely inhibited by the glucose levels that are typically found in L. plantarum growth media (1 to 2% [vol/vol] glucose). In contrast, comparable mannitol levels did not inhibit the reduction of nitrate. L. plantarum reduced nitrate with concomitant formation of nitrite and ammonia. Genes that encode a bd-type cytochrome (cydABCD) and a nitrate reductase (narGHJI) were identified in the genome of L. plantarum. The narGHJI operon is part of a cluster of genes that includes the molybdopterin cofactor biosynthesis genes and narK. Besides a menaquinone source, isogenic mutants revealed that cydA and ndh1 are required for the aerobic-respiration-like response and narG for nitrate reduction. The ndh1 mutant was still able to reduce nitrate. The existence of a nonredundant branched electron transport chain in L. plantarum WCFS1 that is capable of using oxygen or nitrate as a terminal electron acceptor is proposed.

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Crossref · 10.1128/aem.00147-09

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Research works printed but not linked: 3

  1. Arneth, W., and B. Herold. 1988. Nitrat/Nitrit-Bestimmung in Wurstwaren nach enzymatischer Reduction. Fleischwirtschaft68:761-764.
  2. Beutler, H.-O., B. Wurst, and S. Fisher. 1986. Eine neue Methode zur enzymatischen Bestimmung van Nitrat in Lebensmitteln. Dtsch. Lebensmittel-Rundschau82:283-289.
  3. Kretzschmar, R., and T. Kretschmar. 1988. Enzymatische Nitrat-Bestimmung in kommunalen Abwasser. Vom Abwasser70:119-128.

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Metadata

Authors
Brooijmans, R. J. W., de Vos, W. M., & Hugenholtz, J.
Affiliations
  • TiFN (NL)
  • Department of General Microbiology, Wageningen University and Research Centre, Wageningen, The Netherlands
ORCID
Hugenholtz, J.
Venue
Applied and Environmental Microbiology
Publisher
American Society for Microbiology
Published
2009-06
Volume
75
Issue
11
Pages
3580-3585
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
DOI
10.1128/aem.00147-09
PMC
PMC2687314
OpenAlex
W2036330187
Cited by
103
References
44
Citations by year
2026: 3 · 2025: 6 · 2024: 6 · 2023: 9 · 2022: 8 · 2021: 7 · 2020: 3 · 2019: 9 · 2018: 2 · 2017: 11 · 2016: 5 · 2015: 4 · 2014: 5 · 2013: 6 · 2012: 7
FWCI
3.68
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • Socio-Environmental Systems Modeling (acceptedVersion)
  • Data Archiving and Networked Services (DANS) (submittedVersion, other-oa)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial metabolism and enzyme function
Keywords
Lactobacillus plantarum WCFS1, electron transport chain, aerobic respiration, heme, vitamin K2, nitrate reduction, nitrate reductase, narG, terminal electron acceptors, glucose inhibition, nitrite production, ammonia formation
MeSH terms
Aerobiosis, Ammonia, Bacterial Proteins, Electron Transport, Genes, Bacterial, Glucose, Heme, Mannitol, Nitrates, Nitrites, Oxidation-Reduction, Oxygen, Gene Order, Vitamin K 2, Lactiplantibacillus plantarum, Gene Knockout Techniques

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