The DOI matches its Crossref record; 36 of the 36 references it cites were found in a registry or an index.
An Engineered Strong Promoter for Streptomycetes
Open accessAbstract
Well-characterized promoters are essential tools for metabolic engineering and synthetic biology. In Streptomyces coelicolor , the native kasO p is a temporally expressed promoter strictly controlled by two regulators, ScbR and ScbR2. In this work, first, kasO p was engineered to remove a common binding site of ScbR and ScbR2 upstream of its core region, thus generating a stronger promoter, kasO p 3 . Second, another ScbR binding site internal to the kasO p 3 core promoter region was abolished by random mutation and screening of the mutant library to obtain the strongest promoter, kasO p* (where the asterisk is used to distinguish the engineered promoter from the native promoter). The activities of kasO p* were compared with those of two known strong promoters, ermE p* and SF14p, in three Streptomyces species. kasO p* showed the highest activity at the transcription and protein levels in all three hosts. Furthermore, relative to ermE p* and SF14p, kasO p* was shown to confer the highest actinorhodin production level when used to drive the expression of actII -ORF4 in S. coelicolor . Therefore, kasO p* is a simple and well-defined strong promoter useful for gene overexpression in streptomycetes.
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Cites
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Cited by
CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
Zhang, M. M., Wong, F. T., Wang, Y., Luo, S., Lim, Y. H., Heng, E., Yeo, W. L., Cobb, R. E., Enghiad, B., Ang, E. L., et al. (2017) · Nature Chemical Biology · doi:10.1038/nchembio.2341 · Cited by 304
Molecular Basis of Stationary Phase Survival and Applications
Jaishankar, J., & Srivastava, P. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.02000 · Cited by 282
Metadata
- Authors
- Wang, W., Li, X., Wang, J., Xiang, S., Feng, X., & Yang, K.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2013-07-15
- Volume
- 79
- Issue
- 14
- Pages
- 4484-4492
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2073204088
- Cited by
- 310
- References
- 36
- FWCI
- 5.86
- Type
- journal article
- Language
- en
- Open access
- green
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- Wang, W., Li, X., Wang, J., Xiang, S., Feng, X., & Yang, K. (2013). An Engineered Strong Promoter for Streptomycetes. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.00985-13
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