The DOI matches its Crossref record; 14 of the 14 references it cites were found in a registry or an index.
Promoters largely determine the efficiency of repressor action.
Open accessAbstract
Operator sequence and repressor protein regulate the activity of the lac promoter over a greater than 1000-fold range. Combinations of the lac operator with other promoter sequences, however, differ vastly in the level of repression. The data presented show that the extent of repression is determined largely by the rates of complex formation of the competing systems operator-repressor and promoter-RNA polymerase and by the rate at which RNA polymerase clears the promoter. Moreover, up to 70-fold differences in the level of repression were found when the operator was placed in different positions within the promoter sequence. A kinetic model is proposed that explains the observed effects and that allows predictions on promoters controlled by negatively acting elements.
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Cites
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Self-citationThe interaction of RNA polymerase and lac repressor with the lac control region
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[76] Bacteriophage λ repressor and cro protein: Interactions with operator DNA
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Cited by
Recombinant protein expression in Escherichia coli: advances and challenges
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Biofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
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Phagocytic processing of bacterial antigens for class I MHC presentation to T cells
Pfeifer, J. D., Wick, M. J., Roberts, R. L., Findlay, K., Normark, S. J., & Harding, C. V. (1993) · Nature · doi:10.1038/361359a0 · Cited by 615
Control site location and transcriptional regulation in Escherichia coli
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Experimental reproducibility in flow-chamber biofilms
Heydorn, A., Ersbøll, B. K., Hentzer, M., Parsek, M. R., Givskov, M., & Molin, S. (2000) · Microbiology · doi:10.1099/00221287-146-10-2409 · Cited by 264
Metadata
- Authors
- Lanzer, M., & Bujard, H.
- Venue
- Proceedings of the National Academy of Sciences
- Published
- 1988-12
- Volume
- 85
- Issue
- 23
- Pages
- 8973-8977
- ISSN
- 0027-8424 (ISSN-L)
- 1091-6490
- OpenAlex
- W2021539568
- Cited by
- 351
- References
- 14
- FWCI
- 4.63
- Type
- journal article
- Language
- en
- Open access
- green
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
