The DOI matches its Crossref record; 20 of the 20 references it cites were found in a registry or an index.
CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters
Open accessAbstract
Here we report an efficient CRISPR-Cas9 knock-in strategy to activate silent biosynthetic gene clusters (BGCs) in streptomycetes. We applied this one-step strategy to activate multiple BGCs of different classes in five Streptomyces species and triggered the production of unique metabolites, including a novel pentangular type II polyketide in Streptomyces viridochromogenes. This potentially scalable strategy complements existing activation approaches and facilitates discovery efforts to uncover new compounds with interesting bioactivities.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
The new frontier of genome engineering with CRISPR-Cas9
Doudna, J. A., & Charpentier, E. (2014) · Science · doi:10.1126/science.1258096 · Cited by 7,062
CRISPR-Cas systems for editing, regulating and targeting genomes
Sander, J. D., & Joung, J. K. (2014) · Nature Biotechnology · doi:10.1038/nbt.2842 · Cited by 3,153
antiSMASH 3.0—a comprehensive resource for the genome mining of biosynthetic gene clusters
Weber, T., Blin, K., Duddela, S., Krug, D., Kim, H. U., Bruccoleri, R., Lee, S. Y., Fischbach, M. A., Müller, R., Wohlleben, W., et al. (2015) · Nucleic Acids Research · doi:10.1093/nar/gkv437 · Cited by 1,868
Inhibitors of the Nonmevalonate Pathway of Isoprenoid Biosynthesis as Antimalarial Drugs
Jomaa, H., Wiesner, J., Sanderbrand, S., Altincicek, B., Weidemeyer, C., Hintz, M., Türbachova, I., Eberl, M., Zeidler, J., Lichtenthaler, H. K., et al. (1999) · Science · doi:10.1126/science.285.5433.1573 · Cited by 1,173
Discovery of microbial natural products by activation of silent biosynthetic gene clusters
Rutledge, P. J., & Challis, G. L. (2015) · Nature Reviews Microbiology · doi:10.1038/nrmicro3496 · Cited by 986
Cobb, R. E., Wang, Y., & Zhao, H. (2015) · ACS Synthetic Biology · doi:10.1021/sb500351f · Cited by 570
Self-citationCRISPR-Cas9 Based Engineering of Actinomycetal Genomes
Tong, Y., Charusanti, P., Zhang, L., Weber, T., & Lee, S. Y. (2015) · ACS Synthetic Biology · doi:10.1021/acssynbio.5b00038 · Cited by 432
An Engineered Strong Promoter for Streptomycetes
Wang, W., Li, X., Wang, J., Xiang, S., Feng, X., & Yang, K. (2013) · Applied and Environmental Microbiology · doi:10.1128/aem.00985-13 · Cited by 310
One-step high-efficiency CRISPR/Cas9-mediated genome editing in Streptomyces
Huang, H., Zheng, G., Jiang, W., Hu, H., & Lu, Y. (2015) · Acta Biochimica et Biophysica Sinica · doi:10.1093/abbs/gmv007 · Cited by 289
Luo, Y., Huang, H., Liang, J., Wang, M., Lu, L., Shao, Z., Cobb, R. E., & Zhao, H. (2013) · Nature Communications · doi:10.1038/ncomms3894 · Cited by 259
Self-citation
Cited by
Application of different types of CRISPR/Cas-based systems in bacteria
Liu, Z., Dong, H., Cui, Y., Cong, L., & Zhang, D. (2020) · Microbial Cell Factories · doi:10.1186/s12934-020-01431-z · Cited by 185
An isotopic labeling approach linking natural products with biosynthetic gene clusters
McCaughey, C. S., van Santen, J. A., van der Hooft, J. J. J., Medema, M. H., & Linington, R. G. (2022) · Nature Chemical Biology · doi:10.1038/s41589-021-00949-6 · Cited by 39
Metadata
- Authors
- Zhang, M. M., Wong, F. T., Wang, Y., Luo, S., Lim, Y. H., Heng, E., Yeo, W. L., Cobb, R. E., Enghiad, B., Ang, E. L., & Zhao, H.
- Venue
- Nature Chemical Biology
- Published
- 2017-04-10
- Volume
- 13
- Issue
- 6
- Pages
- 607-609
- ISSN
- 1552-4450 (ISSN-L)
- 1552-4469
- OpenAlex
- W2607417244
- Cited by
- 304
- References
- 20
- FWCI
- 22.08
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters. https://app.cochat.ai/papers/10.1038/nchembio.2341
- The paper
- Zhang, M. M., Wong, F. T., Wang, Y., Luo, S., Lim, Y. H., Heng, E., Yeo, W. L., Cobb, R. E., Enghiad, B., Ang, E. L., & Zhao, H. (2017). CRISPR–Cas9 strategy for activation of silent Streptomyces biosynthetic gene clusters. Nature Chemical Biology. https://doi.org/10.1038/nchembio.2341
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
