TiO 2 Photocatalysis Causes DNA Damage via Fenton Reaction-Generated Hydroxyl Radicals during the Recovery Period
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Here, we show that resistance of Escherichia coli to TiO 2 photocatalysis involves defenses against reactive oxygen species. Results support the idea that TiO 2 photocatalysis generates damage which later becomes deleterious during recovery. We found this to be partly due to DNA attack via hydroxyl radicals generated by the Fenton reaction during recovery.
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UVA-excited TiO2 generates singlet oxygen, superoxide, and highly reactive hydroxyl radicals.
“UVA-excited TiO 2 has been shown to generate 1 O 2 (189), O 2) and also highly reactive • OH either through reduction of surface hydroxyl group by valence bond holes (190) or by Fenton type reactions (191).”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [191]
Photoexcited TiO2 exhibits strong bactericidal activity and genotoxicity on various cells mostly as result of oxidative stress.
“Photoexcited TiO 2 exhibits strong bactericidal activity (191)(192)(193)(194) and genotoxicity (195)(196)(197)(198) on various cells as mostly the result of oxidative stress.”
From Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup> (2012) · cites this paper as [191]
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Cites
Imlay, J. A. (2003) · Annual Review of Microbiology · doi:10.1146/annurev.micro.57.030502.090938 · Cited by 2,206 · see citing works
DNA damage and oxygen radical toxicity
Imlay, J. A., & Linn, S. (1988) · Science · doi:10.1126/science.3287616 · Cited by 1,974 · see citing works
FORMATION OF HALOFORMS DURING CHLORINATION OF NATURAL WATERS
Rook, J. J. (1974) · Cited by 1,600 · see citing works
Oxidative stress in bacteria and protein damage by reactive oxygen species.
Cabiscol, E., Tamarit, J., & Ros, J. (2000) · PubMed · Cited by 1,417 · see citing works
Biochemistry of homologous recombination in Escherichia coli
Kowalczykowski, S. C., Dixon, D. A., Eggleston, A. K., Lauder, S., & Rehrauer, W. M. (1994) · Microbiological Reviews · doi:10.1128/mr.58.3.401-465.1994 · Cited by 1,250 · see citing works
The Occurrence of Organohalides in Chlorinated Drinking Waters
Bellar, T. A., Lichtenberg, J. J., & Kroner, R. C. (1974) · American Water Works Association · doi:10.1002/j.1551-8833.1974.tb02129.x · Cited by 1,151 · see citing works
Nieuwenhuijsen, M. J., Toledano, M. B., Eaton, N. E., Fawell, J., & Elliott, P. (2000) · Occupational and Environmental Medicine · doi:10.1136/oem.57.2.73 · Cited by 591 · see citing works
Cho, M., Chung, H.-M., Choi, W., & Yoon, J. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.1.270-275.2005 · Cited by 521 · see citing works
Inactivation of Escherichia coli by titanium dioxide photocatalytic oxidation
Ireland, J. C., Klostermann, P., Rice, E. W., & Clark, R. M. (1993) · Applied and Environmental Microbiology · doi:10.1128/aem.59.5.1668-1670.1993 · Cited by 445 · see citing works
Photocatalytic inactivation of coliform bacteria and viruses in secondary wastewater effluent
Watts, R. J., Kong, S., Orr, M. P., et al. (1995) · Water Research · doi:10.1016/0043-1354(94)e0122-m · Cited by 348 · see citing works
Dukan, S., & Touati, D. (1996) · Journal of Bacteriology · doi:10.1128/jb.178.21.6145-6150.1996 · Cited by 289 · see citing works
Self-citationGogniat, G., Thyssen, M., Denis, M., Pulgarin, C. O., & Dukan, S. (2006) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.2006.00190.x · Cited by 269 · see citing works
Self-citationThe photocatalytic removal of bacterial pollutants from drinking water
Dunlop, P., Byrne, J., Manga, N., et al. (2002) · Journal of Photochemistry and Photobiology A Chemistry · doi:10.1016/s1010-6030(02)00063-1 · Cited by 254 · see citing works
Oxidative Stress Defense and Deterioration of Growth-arrested Escherichia coli Cells
Dukan, S., & Nyström, T. (1999) · Journal of Biological Chemistry · doi:10.1074/jbc.274.37.26027 · Cited by 228 · see citing works
Self-citationPhotocatalytic bactericidal effect of TiO2 on Enterobacter cloacae
Ibáñez, J. A., Litter, M. I., & Pizarro, R. A. (2003) · Journal of Photochemistry and Photobiology A Chemistry · doi:10.1016/s1010-6030(03)00074-1 · Cited by 220 · see citing works
Superoxide and iron: Partners in crime
Liochev, S. I., & Fridovich, I. (1999) · International Union of Biochemistry and Molecular Biology: Life · doi:10.1080/152165499307161 · Cited by 219 · see citing works
TolC Is Involved in Enterobactin Efflux across the Outer Membrane of Escherichia coli
Bleuel, C., Große, C., Taudte, N., Scherer, J., Wesenberg, D., Krauss, G.-J., Nies, D. H., & Grass, G. (2005) · Journal of Bacteriology · doi:10.1128/jb.187.19.6701-6707.2005 · Cited by 180 · see citing works
The photocatalytic disinfection of urban waste waters
Melián, J. A. H., Doña-Rodrı́guez, J. M., Suárez, A. V., et al. (2000) · Chemosphere · doi:10.1016/s0045-6535(99)00502-0 · Cited by 179 · see citing works
TiO2-photocatalysis as a tertiary treatment of naturally treated wastewater
Araña, J., Melián, J. A. H., Doña-Rodrı́guez, J. M., Dı́az, O. G., Viera, A., Peña, J. P., Sosa, P. M. M., & Jiménez, V. (2002) · Catalysis Today · doi:10.1016/s0920-5861(02)00226-2 · Cited by 139 · see citing works
McDonald, L. C., Hackney, C. R., & Ray, B. (1983) · Applied and Environmental Microbiology · doi:10.1128/aem.45.2.360-365.1983 · Cited by 121 · see citing works
Role of escherichia coli rpos and associated genes in defense against oxidative damage
Eisenstark, A., Calcutt, M. J., Becker‐Hapak, M., & Ivanova, A. (1996) · Free Radical Biology and Medicine · doi:10.1016/s0891-5849(96)00154-2 · Cited by 114 · see citing works
Repair and Enumeration of Injured Coliforms by a Plating Procedure
Speck, M. L., Ray, B., & Read, R. B. (1975) · Applied Microbiology · doi:10.1128/am.29.4.549-550.1975 · Cited by 110 · see citing works
Reactive Oxygen Species Are Partially Involved in the Bacteriocidal Action of Hypochlorous Acid
Dukan, S., Belkin, S., & Touati, D. (1999) · Archives of Biochemistry and Biophysics · doi:10.1006/abbi.1999.1265 · Cited by 104 · see citing works
Self-citationDukan, S., Lévi, Y., & Touati, D. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.11.4204-4209.1997 · Cited by 67 · see citing works
Self-citationDubrac, S., & Touati, D. (2002) · Microbiology · doi:10.1099/00221287-148-1-147 · Cited by 66 · see citing works
Induction of a Global Stress Response during the First Step of Escherichia coli Plate Growth
Cuny, C., Lesbats, M., & Dukan, S. (2006) · Applied and Environmental Microbiology · doi:10.1128/aem.01874-06 · Cited by 63 · see citing works
Self-citationEffect of Rehydration on Recovery, Repair, and Growth of Injured Freeze-Dried Salmonella anatum
Ray, B., Jezeski, J. J., & Busta, F. F. (1971) · Applied Microbiology · doi:10.1128/am.22.2.184-189.1971 · Cited by 58 · see citing works
The Effect of Catalase on Recovery of Heat-injured DNA-repair Mutants of Escherichia coli
Mackey, B. M., & Seymour, D. A. (1987) · Microbiology · doi:10.1099/00221287-133-6-1601 · Cited by 42 · see citing works
Resuscitation effects of catalase on airborne bacteria
Marthi, B., Shaffer, B. T., Lighthart, B., et al. (1991) · Applied and Environmental Microbiology · doi:10.1128/aem.57.9.2775-2776.1991 · Cited by 38 · see citing works
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Photoinduced Damage to Cellular DNA: Direct and Photosensitized Reactions<sup>†</sup>
Cadet, J., Mouret, S., Ravanat, J., & Douki, T. (2012) · Photochemistry and Photobiology · doi:10.1111/j.1751-1097.2012.01200.x · Cited by 300 · see citing works
Pinto, A. V., Deodato, E. L., Cardoso, J. S., Oliveira, E. F., Machado, S. L., Toma, H. K., Leitão, A. C., & de Pádula, M. (2010) · Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis · doi:10.1016/j.mrfmmm.2010.02.003 · Cited by 25 · see citing works
- Authors
- Gogniat, G., & Dukan, S.
- Affiliations
- Laboratoire de Chimie Bactérienne, UPR 9043, Université de la Méditerranée, CNRS, 31 Chemin Joseph Aiguier, 13402 Marseille Cedex 20, France
- Centre National de la Recherche Scientifique (FR)
- Corresponding author
- Dukan, Sam
- ORCID
- Dukan, Sam
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2007-12
- Volume
- 73
- Issue
- 23
- Pages
- 7740-7743
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC2168052
- OpenAlex
- W2144481287
- Cited by
- 140
- References
- 29
- Citations by year
- 2026: 3 · 2025: 5 · 2024: 5 · 2023: 3 · 2022: 7 · 2021: 7 · 2020: 14 · 2019: 9 · 2018: 14 · 2017: 10 · 2016: 11 · 2015: 12 · 2014: 10 · 2013: 7 · 2012: 11
- FWCI
- 1.76
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Physical Sciences › Energy › Renewable Energy, Sustainability and the Environment › TiO2 Photocatalysis and Solar Cells
- Physical Sciences › Environmental Science › Water Science and Technology › Advanced oxidation water treatment
- Health Sciences › Medicine › Pulmonary and Respiratory Medicine › Photodynamic Therapy Research Studies
- Keywords
- TiO2 photocatalysis, DNA damage, hydroxyl radicals, Fenton reaction, reactive oxygen species, Escherichia coli, bacterial resistance, convalescence, photocatalytic degradation
- MeSH terms
- DNA Damage, DNA, Bacterial, Escherichia coli, Photochemistry, Titanium, Reactive Oxygen Species, Hydroxyl Radical
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