The DOI matches its Crossref record.
Facilitation of Robust Growth of Prochlorococcus Colonies and Dilute Liquid Cultures by “Helper” Heterotrophic Bacteria
Open accessAbstract
Axenic (pure) cultures of marine unicellular cyanobacteria of the Prochlorococcus genus grow efficiently only if the inoculation concentration is large; colonies form on semisolid medium at low efficiencies. In this work, we describe a novel method for growing Prochlorococcus colonies on semisolid agar that improves the level of recovery to approximately 100%. Prochlorococcus grows robustly at low cell concentrations, in liquid or on solid medium, when cocultured with marine heterotrophic bacteria. Once the Prochlorococcus cell concentration surpasses a critical threshold, the “helper” heterotrophs can be eliminated with antibiotics to produce axenic cultures. Our preliminary evidence suggests that one mechanism by which the heterotrophs help Prochlorococcus is the reduction of oxidative stress.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cited by
Stewart, E. J. (2012) · Journal of Bacteriology · doi:10.1128/jb.00345-12 · Cited by 1,161
Widespread Production of Extracellular Superoxide by Heterotrophic Bacteria
Diaz, J. M., Hansel, C. M., Voelker, B. M., Mendes, C. M., Andeer, P. F., & Zhang, T. (2013) · Science · doi:10.1126/science.1237331 · Cited by 364
Prochlorococcus: Advantages and Limits of Minimalism
Partensky, F., & Garczarek, L. (2010) · Annual Review of Marine Science · doi:10.1146/annurev-marine-120308-081034 · Cited by 294
Black Queen evolution: the role of leakiness in structuring microbial communities
Morris, J. J. (2015) · Trends in Genetics · doi:10.1016/j.tig.2015.05.004 · Cited by 207
Self-citationThe energy expansions of evolution
Judson, O. P. (2017) · Nature Ecology & Evolution · doi:10.1038/s41559-017-0138 · Cited by 118
Metadata
- Authors
- Morris, J. J., Kirkegaard, R., Szul, M. J., Johnson, Z. I., & Zinser, E. R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2008-07-15
- Volume
- 74
- Issue
- 14
- Pages
- 4530-4534
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2101034323
- Cited by
- 298
- References
- 0
- FWCI
- 6.21
- Type
- journal article
- Language
- en
- Open access
- green
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: Facilitation of Robust Growth of Prochlorococcus Colonies and Dilute Liquid Cultures by “Helper” Heterotrophic Bacteria. https://app.cochat.ai/papers/10.1128/aem.02479-07
- The paper
- Morris, J. J., Kirkegaard, R., Szul, M. J., Johnson, Z. I., & Zinser, E. R. (2008). Facilitation of Robust Growth of Prochlorococcus Colonies and Dilute Liquid Cultures by “Helper” Heterotrophic Bacteria. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.02479-07
Data sources
Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
