The DOI matches its Crossref record; 30 of the 30 references it cites were found in a registry or an index.
Functional Expression of a Bacterial Xylose Isomerase in Saccharomyces cerevisiae
Open accessAbstract
In industrial fermentation processes, the yeast Saccharomyces cerevisiae is commonly used for ethanol production. However, it lacks the ability to ferment pentose sugars like d -xylose and l -arabinose. Heterologous expression of a xylose isomerase (XI) would enable yeast cells to metabolize xylose. However, many attempts to express a prokaryotic XI with high activity in S. cerevisiae have failed so far. We have screened nucleic acid databases for sequences encoding putative XIs and finally were able to clone and successfully express a highly active new kind of XI from the anaerobic bacterium Clostridium phytofermentans in S. cerevisiae . Heterologous expression of this enzyme confers on the yeast cells the ability to metabolize d -xylose and to use it as the sole carbon and energy source. The new enzyme has low sequence similarities to the XIs from Piromyces sp. strain E2 and Thermus thermophilus , which were the only two XIs previously functionally expressed in S. cerevisiae . The activity and kinetic parameters of the new enzyme are comparable to those of the Piromyces XI. Importantly, the new enzyme is far less inhibited by xylitol, which accrues as a side product during xylose fermentation. Furthermore, expression of the gene could be improved by adapting its codon usage to that of the highly expressed glycolytic genes of S. cerevisiae . Expression of the bacterial XI in an industrially employed yeast strain enabled it to grow on xylose and to ferment xylose to ethanol. Thus, our findings provide an excellent starting point for further improvement of xylose fermentation in industrial yeast strains.
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Self-citationGenetically Engineered Saccharomyces Yeast Capable of Effective Cofermentation of Glucose and Xylose
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Self-citationEngineering of yeast hexose transporters to transport d -xylose without inhibition by d -glucose
Farwick, A., Bruder, S., Schadeweg, V., Oreb, M., & Boles, E. (2014) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.1323464111 · Cited by 298
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El-Gendi, H., Saleh, A. K., Badierah, R., Redwan, E. M., El-Maradny, Y. A., & El-Fakharany, E. M. (2021) · Journal of Fungi · doi:10.3390/jof8010023 · Cited by 285
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Metadata
- Authors
- Brat, D., Boles, E., & Wiedemann, B.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2009-04-15
- Volume
- 75
- Issue
- 8
- Pages
- 2304-2311
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1989978741
- Cited by
- 291
- References
- 30
- FWCI
- 12.23
- Type
- journal article
- Language
- en
- Open access
- green
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