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Purification and Characterization of Liposan, a Bioemulsifier from Candida lipolytica
Open accessAbstract
The inducible water-soluble bioemulsifier liposan (M. C. Cirigliano and G. M. Carman, Appl. Environ. Microbiol. 48:747-750, 1984) was purified from the yeast Candida lipolytica. The purification procedure included repeated solvent extractions of a concentrated culture filtrate and Affi-Gel concanavalin A affinity chromatography. The procedure yielded a preparation containing a major band ( M r = 27,600) which stained for protein and carbohydrate upon polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. Liposan is composed of approximately 83% carbohydrate and 17% protein. Acid and enzymatic digestions of the emulsifier revealed that the carbohydrate portion is a heteropolysaccharide consisting of glucose, galactose, galactosamine, and galacturonic acid. Liposan effected and stabilized oil-in-water emulsions with a variety of commercial vegetable oils. Emulsification and stabilization properties of liposan were compared to those of a number of commercial emulsifiers and stabilizers. Images:
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Groenewald, M., Boekhout, T., Neuvéglise, C., Gaillardin, C., van Dijck, P. W. M., & Wyss, M. (2014) · Critical Reviews in Microbiology · doi:10.3109/1040841x.2013.770386 · Cited by 498
Metadata
- Authors
- Cirigliano, M. C., & Carman, G. M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1985-10
- Volume
- 50
- Issue
- 4
- Pages
- 846-850
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2128240398
- Cited by
- 277
- References
- 0
- FWCI
- 3.86
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Purification and Characterization of Liposan, a Bioemulsifier from Candida lipolytica. https://app.cochat.ai/papers/10.1128/aem.50.4.846-850.1985
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- Cirigliano, M. C., & Carman, G. M. (1985). Purification and Characterization of Liposan, a Bioemulsifier from Candida lipolytica. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.50.4.846-850.1985
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