The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation
Open accessAbstract
An electroporation procedure for the plasmid-mediated genetic transformation of intact cells of Streptococcus cremoris and Streptococcus lactis was performed. Ten different strains were transformed. The method was simple and rapid and yielded transformant colonies in 14 to 24 h. The method was optimized for S. lactis LM0230, and transformation frequencies of between 1 × 10 4 and 5 × 10 5 transformants per μg of purified plasmid (pMU1328) were achieved routinely. The optimized procedure involved lysozyme treatment of cells. Transformation of LM0230 occurred at comparable frequencies with pLS1 (4.4 kilobase pair [kbp]), pMU1328 (7.4 kbp), and pAMβ1 (26.5 kbp). Plasmid DNA isolated from transformants had not undergone detectable deletions or rearrangements. Transformation was possible with plasmid DNA which was religated after restriction endonuclease digestion. Phage DNA-dependent transfection of S. lactis LM0230 and S. lactis C6 was also achieved.
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Citations
Cites
A rapid alkaline extraction procedure for screening recombinant plasmid DNA
Birnboim, H. C., & Doly, J. (1979) · Nucleic Acids Research · doi:10.1093/nar/7.6.1513 · Cited by 14,686
Cited by
Holo, H., & Nes, I. F. (1989) · Applied and Environmental Microbiology · doi:10.1128/aem.55.12.3119-3123.1989 · Cited by 1,048
Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation
Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P. (1988) · FEMS Microbiology Letters · doi:10.1111/j.1574-6968.1988.tb03154.x · Cited by 133
Metadata
- Authors
- Powell, I. B., Achen, M. G., Hillier, A. J., & Davidson, B. E.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1988-03
- Volume
- 54
- Issue
- 3
- Pages
- 655-660
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2142716675
- Cited by
- 179
- References
- 1
- FWCI
- 13.68
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Powell, I. B., Achen, M. G., Hillier, A. J., & Davidson, B. E. (1988). A Simple and Rapid Method for Genetic Transformation of Lactic Streptococci by Electroporation. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.54.3.655-660.1988
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