Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation

Oultram, J.D.; Loughlin, M.; Swinfield, T-J.; Brehm, J.K.; Thompson, D.E.; Minton, N.P.

doi:10.1111/j.1574-6968.1988.tb03154.x

Cited by 133Bacterial Genetics and BiotechnologyMicrobial Inactivation MethodsMicrobial Metabolic Engineering and Bioproduction

Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation

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Oultram, J.D., Loughlin, M., Swinfield, T-J., Brehm, J.K., Thompson, D.E., Minton, N.P.·1988·FEMS Microbiology Letters

DOI 10.1111/j.1574-6968.1988.tb03154.x

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Abstract

A method is presented for the introduction of plasmids into Clostridium acetobutylicum ATCC 8052 by electroporation. A plasmid shuttle vector, pMTL500E, which contains the erythromycin resistance gene and replication machinery of plasmid pAMβ1, was constructed and introduced into C. acetobutylicum by electroporation. The vector was then used to introduce a 2.2 kb ClaI/SphI chromosomal fragment from C. pasteurianum into a leucine requiring mutant of C. acetobutylicum, SBA9, where complementation of auxotrophy was observed. Plasmid DNA indistinguishable from that introduced, on the basis of agarose gel electrophoresis, was observed in transformants containing either plasmid.

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Some well-studied non-acetogenic clostridia, such as C. acetobutylicum, C. beijerinckii, C. difficile, and C. botulinum, have transformation protocols that were established as early as 1988.
“Some of the well-studied, non-acetogenic clostridia, such as the ABE model organisms C. acetobutylicum (Mermelstein et al., 1992) and C. beijerinckii (Oultram et al., 1988), and important pathogens, such as C. difficile (Ackermann et al., 2001) and C. botulinum (Zhou and Johnson, 1993), have transformation protocols that were established as early as 1988.”
From Gas Fermentation—A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks (2016) · cites this paper as Oultram et al., 1988

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Authors: Oultram, J. D., Loughlin, M., Swinfield, T.-J., Brehm, J. K., Thompson, D. E., & Minton, N. P.
ORCID: Minton, N.P.
Venue: FEMS Microbiology Letters
Publisher: Oxford University Press (OUP)
Published: 1988-11
Volume: 56
Issue: 1
Pages: 83-88
ISSN: 0378-1097 (ISSN-L)
1574-6968
DOI: 10.1111/j.1574-6968.1988.tb03154.x
OpenAlex: W1979262511
Cited by: 133
References: 24
Citations by year: 2024: 1 · 2023: 2 · 2022: 3 · 2021: 1 · 2019: 2 · 2017: 3 · 2016: 8 · 2015: 5 · 2014: 7 · 2013: 3 · 2012: 4
FWCI: 3.55
Type: journal article
Language: en
Open access: bronze
doi.org
Locations: FEMS Microbiology Letters (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Inactivation Methods
Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
Keywords: Clostridium acetobutylicum, electroporation, plasmid transformation, shuttle vector, erythromycin resistance, leucine auxotrophy

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Introduction of plasmids into whole cells of Clostridium acetobutylicum by electroporation

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