The DOI matches its Crossref record; 24 of the 24 references it cites were found in a registry or an index.
Efficacy of Phospholipid Analysis in Determining Microbial Biomass in Sediments
Open accessAbstract
Improvements in the analysis of lipid-bound phosphates resulted in a simplified and sensitive method for determining microbial biomass in sediments. Sensitivity was enhanced over previous methods by use of a dye, malachite green, which when complexed with phosphomolybdate at low pH has a high extinction coefficient (at 610 nm). The use of a persulfate oxidation technique to liberate phosphate from lipids increased the simplicity and safety of the method relative to the traditional perchloric acid digestions. The modified method was both accurate (yielding quantitative recoveries of cells added to sediments) and precise (coefficient of variation of less than 5% for cells and sediments). A comparison with an epifluorescence technique indicated that the analysis of lipid-bound phosphate was more rapid and less tedious and could be successfully applied to a wider variety of sediment types. An estimate of the lipid-bound phosphate-to-carbon conversion factor based on a diverse enrichment culture from sediments suggested that previous factors for pure cultures may have been too low.
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Cites
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An improved assay for nanomole amounts of inorganic phosphate
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Determination of the sedimentary microbial biomass by extractible lipid phosphate
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Relationships between Biovolume and Biomass of Naturally Derived Marine Bacterioplankton
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A new micromethod for the colorimetric determination of inorganic phosphate
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Determination of total nitrogen in aqueous samples using persulfate digestion1
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Bacterial Biovolume and Biomass Estimations
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Cited by
Microbial Cellulose Utilization: Fundamentals and Biotechnology
Lynd, L. R., Weimer, P. J., van Zyl, W. H., & Pretorius, I. S. (2002) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.66.3.506-577.2002 · Cited by 4,726
Microbial biomass measured as total lipid phosphate in soils of different organic content
Frostegård, Å., Tunlid, A., & Bååth, E. (1991) · Journal of Microbiological Methods · doi:10.1016/0167-7012(91)90018-l · Cited by 960
A simplified diphenylamine colorimetric method for growth quantification
Zhao, Y., Xiang, S., Dai, X., & Yang, K. (2013) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-013-4893-y · Cited by 64
A Technological Understanding of Biofilm Detection Techniques: A Review
Achinas, S., Yska, S. K., Charalampogiannis, N., Krooneman, J., & Euverink, G. J. W. (2020) · Materials · doi:10.3390/ma13143147 · Cited by 46
Atoloye, I. A., Adesina, I. S., Sharma, H., Subedi, K., Liang, C.-L. (., Shahbazi, A., & Bhowmik, A. (2022) · PLOS ONE · doi:10.1371/journal.pone.0264620 · Cited by 16
“8) buffer, with PLFA eluted from silicic acid columns in methanol, following removal of non-polar lipids by washing with chloroform and acetone [23].”
Method
Metadata
- Authors
- Findlay, R. H., King, G. M., & Watling, L.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1989-11
- Volume
- 55
- Issue
- 11
- Pages
- 2888-2893
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1580992421
- Cited by
- 400
- References
- 24
- FWCI
- 12.53
- Type
- journal article
- Language
- en
- Open access
- bronze
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- CoChat. (2026). Citation check: Efficacy of Phospholipid Analysis in Determining Microbial Biomass in Sediments. https://app.cochat.ai/papers/10.1128/aem.55.11.2888-2893.1989
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- Findlay, R. H., King, G. M., & Watling, L. (1989). Efficacy of Phospholipid Analysis in Determining Microbial Biomass in Sediments. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.55.11.2888-2893.1989
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Bibliographic data: Semantic Scholar, OpenAlex, Crossref.
Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.
