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A Technological Understanding of Biofilm Detection Techniques: A Review
Abstract
Biofouling is a persistent problem in almost any water-based application in several industries. To eradicate biofouling-related problems in bioreactors, the detection of biofilms is necessary. The current literature does not provide clear supportive information on selecting biofilm detection techniques that can be applied to detect biofouling within bioreactors. Therefore, this research aims to review all available biofilm detection techniques and analyze their characteristic properties to provide a comparative assessment that researchers can use to find a suitable biofilm detection technique to investigate their biofilms. In addition, it discusses the confluence of common bioreactor fabrication materials in biofilm formation.
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![Figure 1 A visualization of the five phenomena of biofilm formation. The figure indicates the conditions of the medium and the specific surface on which microbial adhesion occurs; the initial adherence of microorganisms to the surface; the proliferation (or else microcolony formation); and the maturation of the biofilm architecture with the presence of the polymeric matrix and its dispersion [7,9].](/paper-figures/a3deac32dde343e296c9102fdba86b48/a-technological-understanding-of-biofilm-detection-techniques-a-review-figure-1.jpg)
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“Similarly, reverse transcription-polymerase chain reaction evaluates biofilm-related gene expression but does not offer insights into the spatial organization of the EPS matrix and biofilm morphology.45-47”
From SEMTWIST Quantification of Biofilm Infection in Human Chronic Wound Using Scanning Electron Microscopy and Machine Learning (2025) · cites this paper as [47]
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Cited by
Sorted by citations
Cheah, Y. T., & Chan, D. J. C. (2022) · Journal of Applied Microbiology · doi:10.1111/jam.15455 · Cited by 108
Singh, S., Muniz De Oliveira, F., Wang, C., Kumar, M., Xuan, Y., DeMazumder, D., Sen, C. K., & Roy, S. (2025) · Advances in Wound Care · doi:10.1089/wound.2024.0291 · Cited by 6
Metadata
- Authors
- Achinas, S., Yska, S. K., Charalampogiannis, N., Krooneman, J., & Euverink, G. J. W.
- Venue
- Materials
- Publisher
- MDPI AG
- Published
- 2020-07-15
- Volume
- 13
- Issue
- 14
- Pages
- 3147
- ISSN
- 1996-1944
- PMC
- PMC7412299
- Cited by
- 46
- References
- 0
- Language
- en
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
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How to cite
Cite CoChat for the checks on this page, and the paper for its own findings.
- This page
- CoChat. (2026). Citation check: A Technological Understanding of Biofilm Detection Techniques: A Review. https://app.cochat.ai/papers/10.3390/ma13143147
- The paper
- Achinas, S., Yska, S. K., Charalampogiannis, N., Krooneman, J., & Euverink, G. J. W. (2020). A Technological Understanding of Biofilm Detection Techniques: A Review. Materials. https://doi.org/10.3390/ma13143147