The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples
Open accessAbstract
To detect low levels of microorganisms in environmental samples by using polymerase chain reaction (PCR)-gene probe detection, samples were concentrated by filtration. Fluoropore (Millipore Corp.) filters were compatible with PCR DNA amplification, whereas various other filters including nitrocellulose and cellulose acetate filters inhibited PCR amplification. By concentrating cells on Fluoropore filters and releasing the DNA by freeze-thaw cycling, PCR DNA amplification could be performed without removing the filter. Concentration with Fluoropore FHLP and FGLP filters permitted the detection of single cells of microorganisms in 100-ml samples by PCR-gene probes.
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Cites
Primer-Directed Enzymatic Amplification of DNA with a Thermostable DNA Polymerase
Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., & Erlich, H. A. (1988) · Science · doi:10.1126/science.2448875 · Cited by 17,719
Cited by
Chellaiah, E. R., Ravi, P., & Uthandakalaipandian, R. (2021) · Folia Microbiologica · doi:10.1007/s12223-021-00867-z · Cited by 16
Metadata
- Authors
- Bej, A. K., Mahbubani, M. H., Dicesare, J. L., & Atlas, R. M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-12
- Volume
- 57
- Issue
- 12
- Pages
- 3529-3534
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1574617738
- Cited by
- 204
- References
- 1
- FWCI
- 2.92
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Bej, A. K., Mahbubani, M. H., Dicesare, J. L., & Atlas, R. M. (1991). Polymerase chain reaction-gene probe detection of microorganisms by using filter-concentrated samples. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.57.12.3529-3534.1991
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