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Production of Alkaline Protease by a New Aspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent
Open accessAbstract
The production of alkaline protease by an Aspergillus flavus strain isolated in our laboratory by solid-substrate fermentation for use as a depilation agent and the influence of various factors on enzyme production are reported. The optimum conditions for maximum production were a growth temperature of 32°C, 63% substrate moisture, and a growth period of 48 h. Enrichment with corn steep liquor or Casitone increased productivity. Scaling-up experiments indicated that flask-scale results could be reproduced at 1 and 30 kg of substrate. The enzyme preparation exhibited maximum activity at both pH 7.5 and pH 9.5. The use of this enzyme as a depilation agent was confirmed by experiments in a tannery.
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Cited by
A biotechnology perspective of fungal proteases
Souza, P. M. D., Bittencourt, M. L. D. A., Caprara, C. C., Freitas, M. D., Almeida, R. P. C. D., Silveira, D., Fonseca, Y. M., Ferreira Filho, E. X., Pessoa Junior, A., & Magalhães, P. O. (2015) · Brazilian Journal of Microbiology · doi:10.1590/s1517-838246220140359 · Cited by 345
Beaulieu, M., Beaulieu, Y., Melinard, J., Pandian, S., & Goulet, J. (1995) · Applied and Environmental Microbiology · doi:10.1128/aem.61.1.165-169.1995 · Cited by 94
Irfan, M., Rauf, A., Syed, Q., Nadeem, M., & Baig, S. (2011) · International Journal for Agro Veterinary and Medical Sciences · doi:10.5455/ijavms.20110215080443 · Cited by 8
Metadata
- Authors
- Malathi, S., & Chakraborty, R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-03
- Volume
- 57
- Issue
- 3
- Pages
- 712-716
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2115851465
- Cited by
- 185
- References
- 0
- FWCI
- 3.17
- Type
- journal article
- Language
- en
- Open access
- green
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- CoChat. (2026). Citation check: Production of Alkaline Protease by a New Aspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent. https://app.cochat.ai/papers/10.1128/aem.57.3.712-716.1991
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- Malathi, S., & Chakraborty, R. (1991). Production of Alkaline Protease by a New Aspergillus flavus Isolate under Solid-Substrate Fermentation Conditions for Use as a Depilation Agent. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.57.3.712-716.1991
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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