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Cited by 8Enzyme Production and CharacterizationBiofuel production and bioconversionFungal and yeast genetics research

Exploitation of Different Agro-residues for Acid protease Production by Rhizopus sp. in Koji Fermentation

Irfan, Muhammad, Rauf, Abdul, Syed, Quratualain, Nadeem, Muhammad, Baig, Shahjahan·2011·International Journal for Agro Veterinary and Medical Sciences
DOI 10.5455/ijavms.20110215080443
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Abstract

Two different strains of Rhizopus sp. i.e. R. oligosporus -M30 and R. arrhizus -M26 were employed for the production of acid protease using different agro-residues such as rice husk, rice bran, wheat bran, defatted soybean meal and sunflower meal as a sole carbon source. R. oligosporus -M30 gave maximum enzyme level with soybean meal (15.6 U/gds) while R. arrhizus -M26 with sunflower meal (13.6 U/gds) respectively. The optimal conditions for the enzyme production by R .oligosporus -M30 were initial pH of 5.5, temperature 30 oC with 10% of inoculum size for 72hr of fermentation period. When R. arrhizus -M26 was cultivated that exhibit the best enzyme production with the fermentation period of 72h, initial pH 5.0, temperature 33 oC with 5% of inoculum size

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Crossref · 10.5455/ijavms.20110215080443

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23 of 23 references verified0 citation errors · 0 not checkable

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  • The highest protease production (15.6 ± 0.92 U/gds) occurred with soybean meal by R. arrhizus.

    “Irfan et al. (2011) showed that among the different substrates tested, the highest protease production (15.6 ± 0.92 U/gram dry substrate [gds]) occurred with soybean meal by R. arrhizus and 11.7 ± 0.74 U/gds by Rhizopus oligosporus when sunflower meal was used.”

    From Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review. (2025) · cites this paper as Irfan et al., 2011

  • Protease production by Rhizopus oligosporus was 11.7 ± 0.74 U/gds when sunflower meal was used.

    “Irfan et al. (2011) showed that among the different substrates tested, the highest protease production (15.6 ± 0.92 U/gram dry substrate [gds]) occurred with soybean meal by R. arrhizus and 11.7 ± 0.74 U/gds by Rhizopus oligosporus when sunflower meal was used.”

    From Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review. (2025) · cites this paper as Irfan et al., 2011

  • Variations in MCE production may be due to differences in fungal characteristics as well as the specific composition of the preferred substrate for protease production.

    “These variations may be due to differences in fungal characteristics as well as the specific composition of the preferred substrate for protease production (Irfan et al., 2011).”

    From Invited review: Milk-clotting enzymes of microbial origin and their role in cheesemaking-A review. (2025) · cites this paper as Irfan et al., 2011

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Authors
Irfan, M., Rauf, A., Syed, Q., Nadeem, M., & Baig, S.
ORCID
Venue
International Journal for Agro Veterinary and Medical Sciences
Publisher
ScopeMed
Published
2011
Volume
5
Issue
1
Pages
43
ISSN
1999-9666 (ISSN-L)
DOI
10.5455/ijavms.20110215080443
OpenAlex
W2126659905
Cited by
8
References
24
Citations by year
2025: 1 · 2022: 3 · 2021: 1 · 2020: 1 · 2017: 1 · 2014: 1
FWCI
0.27
Type
journal article
Language
en
Open access
closed
Locations
International Journal for Agro Veterinary and Medical Sciences (publishedVersion)
Topics
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
  • Physical Sciences › Engineering › Biomedical Engineering › Biofuel production and bioconversion
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Fungal and yeast genetics research
Keywords
Rhizopus oligosporus, Rhizopus oryzae, koji fermentation, agro-residues, rice husk, rice bran, wheat bran, defatted soybean meal, sunflower meal, carbon source, enzyme production optimization, inoculum size, fermentation temperature, initial pH

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