Production of beta-carotene in Zymomonas mobilis and Agrobacterium tumefaciens by introduction of the biosynthesis genes from Erwinia uredovora
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The Erwinia uredovora crtB, crtE, crtI, and crtY genes required for beta-carotene biosynthesis were introduced by conjugal transfer into an ethanol-producing bacterium, Zymomonas mobilis, and a phytopathogenic bacterium, Agrobacterium tumefaciens, in which no carotenoid is synthesized. The transconjugants of Z. mobilis and A. tumefaciens carrying these genes appeared as yellow colonies and produced 220 and 350 micrograms of beta-carotene per g of dry weight, respectively, in the stationary phase in liquid culture.
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Z. mobilis can be engineered to produce other valuable bioproducts, such as isobutanol and isoprenoids.
“It naturally converts up to 96% of glucose into ethanol and can be engineered to produce other valuable bioproducts, such as isobutanol and isoprenoids (5–11).”
From Physiological and metabolic responses of <i>Zymomonas mobilis</i> to lignocellulosic hydrolysate (2025) · cites this paper as [10]
Z. mobilis naturally converts up to 96% of glucose into ethanol.
“It naturally converts up to 96% of glucose into ethanol and can be engineered to produce other valuable bioproducts, such as isobutanol and isoprenoids (5–11).”
From Physiological and metabolic responses of <i>Zymomonas mobilis</i> to lignocellulosic hydrolysate (2025) · cites this paper as [10]
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Physiological and metabolic responses of <i>Zymomonas mobilis</i> to lignocellulosic hydrolysate
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- Authors
- Misawa, N., Yamano, S., & Ikenaga, H.
- Affiliations
- Kirin (Japan) (JP)
- ORCID
- Misawa, N
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1991-06
- Volume
- 57
- Issue
- 6
- Pages
- 1847-1849
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC183481
- OpenAlex
- W1552989068
- Cited by
- 69
- References
- 18
- Citations by year
- 2026: 1 · 2025: 5 · 2024: 2 · 2022: 1 · 2021: 2 · 2020: 2 · 2019: 1 · 2018: 3 · 2017: 1 · 2016: 1 · 2015: 1 · 2014: 3 · 2013: 1 · 2012: 2
- FWCI
- 2.89
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Microbial Metabolism and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Microbial Metabolic Engineering and Bioproduction
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Plant biochemistry and biosynthesis
- Keywords
- β-carotene biosynthesis, Zymomonas mobilis, Agrobacterium tumefaciens, Erwinia uredovora, crtB, chromium telluride, crtI, carotenoid biosynthesis genes, conjugative transfer, transconjugants, recombinant bacteria, ethanol production
- MeSH terms
- Carotenoids, Conjugation, Genetic, Erwinia, Genes, Bacterial, Gram-Negative Anaerobic Bacteria, Kinetics, Plasmids, Rhizobium, beta Carotene
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