The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Factors involved in adherence of lactobacilli to human Caco-2 cells
Open accessAbstract
A quantitative assay performed with bacterial cells labelled with [3H]thymidine was used to investigate factors involved in the adherence of human isolates Lactobacillus acidophilus BG2FO4 and NCFM/N2 and Lactobacillus gasseri ADH to human Caco-2 intestinal cells. For all three strains, adherence was concentration dependent, greater at acidic pH values, and significantly greater than adherence of a control dairy isolate, Lactobacillus delbrueckii subsp. bulgaricus 1489. Adherence of L. acidophilus BG2FO4 and NCFM/N2 was decreased by protease treatment of the bacterial cells, whereas adherence of L. gasseri ADH either was not affected or was enhanced by protease treatment. Putative surface layer proteins were identified on L. acidophilus BG2FO4 and NCFM/N2 cells but were not involved in adherence. Periodate oxidation of bacterial cell surface carbohydrates significantly reduced adherence of L. gasseri ADH, moderately reduced adherence of L. acidophilus BG2FO4, and had no effect on adherence of L. acidophilus NCFM/N2. These results indicate that Lactobacillus species adhere to human intestinal cells via mechanisms which involve different combinations of carbohydrate and protein factors on the bacterial cell surface. The involvement of a secreted bridging protein, which has been proposed as the primary mediator of adherence of L. acidophilus BG2FO4 in spent culture supernatant (M.-H. Coconnier, T. R. Klaenhammer, S. Kernéis, M.-F. Bernet, and A. L. Servin, Appl. Environ. Microbiol. 58:2034-2039, 1992), was not confirmed in this study. Rather, a pH effect on Caco-2 cells contributed significantly to the adherence of this strain in spent culture supernatant.(ABSTRACT TRUNCATED AT 250 WORDS)
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Citations
Cites
Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells
CHAUVIERE, G., COCONNIER, M.-H., KERNEIS, S., FOURNIAT, J., & SERVIN, A. L. (1992) · Journal of General Microbiology · doi:10.1099/00221287-138-8-1689 · Cited by 249
Cited by
Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens
Servin, A. L. (2004) · FEMS Microbiology Reviews · doi:10.1016/j.femsre.2004.01.003 · Cited by 1,236
Probiotic Mechanisms of Action
Bermudez-Brito, M., Plaza-Díaz, J., Muñoz-Quezada, S., Gómez-Llorente, C., & Gil, A. (2012) · Annals of Nutrition and Metabolism · doi:10.1159/000342079 · Cited by 1,234
Dunne, C., O’Mahony, L., Murphy, L., Thornton, G., Morrissey, D., O’Halloran, S., Feeney, M., Flynn, S., Fitzgerald, G., Daly, C., et al. (2001) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.386s · Cited by 922
Kos, B., Šušković, J., Vuković, S., Šimpraga, M., Frece, J., & Matošić, S. (2003) · Journal of Applied Microbiology · doi:10.1046/j.1365-2672.2003.01915.x · Cited by 880
Taxonomy and important features of probiotic microorganisms in food and nutrition
Holzapfel, W. H., Haberer, P., Geisen, R., Björkroth, J., & Schillinger, U. (2001) · The American Journal of Clinical Nutrition · doi:10.1093/ajcn/73.2.365s · Cited by 759
Metadata
- Authors
- Greene, J. D., & Klaenhammer, T. R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1994-12
- Volume
- 60
- Issue
- 12
- Pages
- 4487-4494
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2133439477
- Cited by
- 298
- References
- 1
- FWCI
- 9.64
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Greene, J. D., & Klaenhammer, T. R. (1994). Factors involved in adherence of lactobacilli to human Caco-2 cells. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.60.12.4487-4494.1994
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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