Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells

CHAUVIERE, G.; COCONNIER, M.-H.; KERNEIS, S.; FOURNIAT, J.; SERVIN, A. L.

doi:10.1099/00221287-138-8-1689

Cited by 249Probiotics and Fermented FoodsReproductive tract infections researchInfant Nutrition and Health

Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells

CHAUVIERE, G., COCONNIER, M.-H., KERNEIS, S., FOURNIAT, J., SERVIN, A. L.·1992·Journal of General Microbiology

DOI 10.1099/00221287-138-8-1689

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Abstract

Twenty-five strains of lactobacilli were tested for their ability to adhere to human enterocyte-like Caco-2 cells in culture. Seven Lactobacillus strains adhered well to the Caco-2 cells, of which three possessed calcium-independent adhesion properties. A high level of calcium-independent adhesion was observed with the human stool isolate Lactobacillus acidophilus strain LB. Scanning electron microscopy revealed that this strain adhered to the apical brush border of the cells. Adhesion increased in parallel with the morphological and functional differentiation of the Caco-2 cells. Two Lactobacillus components were involved in this adhesion. One was protease-resistant and bacterial-surface-associated; the other was heat-stable, extracellular and protease-sensitive.

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Caco-2 cells express morphological and functional differentiation in vitro and show characteristics of mature enterocytes; HT-29 cells show typical epithelial cell morphology and produce large amounts of mucus.
“The Caco-2 cell line express morphological and functional differentiation in vitro and show characteristics of mature enterocytes. In turn, the HT-29 line shows a typical epithelial cell morphology, producing large amounts of mucus (Chauviere et al. 1992; Duary et al. 2011; Sharma and Kanwar 2017).”
From Adhesion and aggregation properties of Lactobacillaceae strains as protection ways against enteropathogenic bacteria (2022) · cites this paper as Chauviere et al. 1992

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Authors: CHAUVIERE, G., COCONNIER, M.-H., KERNEIS, S., FOURNIAT, J., & SERVIN, A. L.
Venue: Journal of General Microbiology
Publisher: Microbiology Society
Published: 1992-08-01
Volume: 138
Issue: 8
Pages: 1689-1696
ISSN: 0022-1287 (ISSN-L)
DOI: 10.1099/00221287-138-8-1689
OpenAlex: W2100511668
Cited by: 249
References: 35
Citations by year: 2025: 4 · 2024: 3 · 2023: 3 · 2022: 10 · 2021: 2 · 2020: 7 · 2019: 6 · 2018: 8 · 2017: 5 · 2016: 6 · 2015: 10 · 2014: 12 · 2013: 11 · 2012: 13
FWCI: 10.59
Type: journal article
Language: en
Open access: closed
Locations: Journal of General Microbiology (publishedVersion)
PubMed (publishedVersion)
Topics: Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
Health Sciences › Nursing › Nutrition and Dietetics › Infant Nutrition and Health
Keywords: Lactobacillus acidophilus, bacterial adhesion, Caco-2 cells, enterocyte differentiation, calcium-independent adhesion, brush border, probiotic bacteria
MeSH terms: Bacterial Adhesion, Binding Sites, Cell Differentiation, Cell Line, Humans, Intestines, Kinetics, Lactobacillus acidophilus

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Adhesion of human Lactobacillus acidophilus strain LB to human enterocyte-like Caco-2 cells

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