The DOI matches its Crossref record; 37 of the 37 references it cites were found in a registry or an index.
Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection
Open accessAbstract
The aim of this study was to compare the antagonistic properties of Lactobacillus casei GG exerted in vitro against Salmonella typhimurium C5 in a cellular model, cultured enterocyte-like Caco-2 cells, to those exerted in vivo in an animal model, C3H/He/Oujco mice. Our results show that a 1-h contact between the invading strain C5 and either the culture or the supernatant of L. casei GG impeded the invasion by the Salmonella strain in Caco-2 cells, without modifying the viability of the strain. After neutralization at pH 7, no inhibition of the invasion by C5 was observed. The antagonistic activity of L. casei GG was examined in C3H/He/Oujco mice orally infected with C5 as follows: (i) L. casei GG was given daily to conventional animals as a probiotic, and (ii) it was given once to germ-free animals in order to study the effect of the population of L. casei GG established in the different segments of the gut. In vivo experiments show that after a single challenge with C5, this strain survives and persists at a higher level in the feces of the untreated conventional mice than in those of the treated group. In L. casei GG germ-free mice, establishment of L. casei GG in the gut significantly delayed the occurrence of 100% mortality of the animals (15 days after C5 challenge versus 9 days in germ-free mice [P < 0.01]). Cecal colonization level and translocation rate of C5 to the mesenteric lymph nodes, spleen, and liver were significantly reduced during the first 2 days post-C5 challenge, although the L. casei GG population level in the gut dramatically decreased in these animals.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“The survival of probiotic bacteria in gastrointestinal ecosystems and their adhesion to intestinal mucosa is considered to be preconditions for temporary colonization, stimulation of the immune system, and resistance to intestinal pathogens (Hudault et al., 1997; Roselli et al., 2005).”
From Adhesion and Colonization of the Probiotic Lactobacillus plantarum HC-2 in the Intestine of Litopenaeus Vannamei Are Associated With Bacterial Surface Proteins (2022) · cites this paper as Hudault et al., 1997
Experimental — accuracy not yet measured
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
A medium for the cultivation of lactobacilli
De MAN, J. C., ROGOSA, M., & SHARPE, M. E. (1960) · Journal of Applied Bacteriology · doi:10.1111/j.1365-2672.1960.tb00188.x · Cited by 5,311
MICROBIAL ECOLOGY OF THE GASTROINTESTINAL TRACT
Savage, D. C. (1977) · Annual Review of Microbiology · doi:10.1146/annurev.mi.31.100177.000543 · Cited by 2,436
Genetics of bacteriocins produced by lactic acid bacteria
Klaenhammer, T. R. (1993) · FEMS Microbiology Reviews · doi:10.1111/j.1574-6976.1993.tb00012.x · Cited by 2,051
Pinto, M. (1983) · HAL · Cited by 1,752
One Hundred and Twenty-Seven Cultured Human Tumor Cell Lines Producing Tumors in Nude Mice23
Fogh, J., Fogh, J. M., & Orfeo, T. (1977) · JNCI Journal of the National Cancer Institute · doi:10.1093/jnci/59.1.221 · Cited by 1,599
Saavedra, J. M., Bauman, N. A., Perman, J. A., Yolken, R. H., Saavedra, J. M., Bauman, N. A., & Oung, I. (1994) · The Lancet · doi:10.1016/s0140-6736(94)91708-6 · Cited by 958
Jones, B. D., Ghori, N., & Falkow, S. (1994) · The Journal of Experimental Medicine · doi:10.1084/jem.180.1.15 · Cited by 883
Bernet‐Camard, M., Brassart, D., Neeser, J., & Servin, A. L. (1994) · Gut · doi:10.1136/gut.35.4.483 · Cited by 751
Kaila, M., Isolauri, E., Soppi, E. T., Virtanen, E., Laine, S., & Arvilommi, H. S. (1992) · Pediatric Research · doi:10.1203/00006450-199208000-00002 · Cited by 700
Isolauri, E., Juntunen, M., Rautanen, T., Sillanaukee, P., & Koivula, T. (1991) · PubMed · Cited by 684
Research works printed but not linked: 1
- Hudault S., Ducluzeau R., Dubos F., Raibaud P., Ghnassia J. C., Griscelli C. Elimination du tube digestif d'un enfant "gnotoxénique" d'une souche de Lactobacillus casei, issue d'une préparation commerciale: démonstration chez des souris "gnotoxéniques" du rôle antagoniste d'une souche de Escherichia coli d'origine humaine. Ann Microbiol (Paris) 1976 Jul;127(1):75–82. [PubMed] [Google Scholar]
Cited by
Sorted by citations
Antagonistic activities of lactobacilli and bifidobacteria against microbial pathogens
Servin, A. L. (2004) · FEMS Microbiology Reviews · doi:10.1016/j.femsre.2004.01.003 · Cited by 1,267
Probiotic bacteria: safety, functional and technological properties
Saarela, M., Mogensen, G., Fondén, R., Mättö, J., & Mattila-Sandholm, T. (2000) · Journal of Biotechnology · doi:10.1016/s0168-1656(00)00375-8 · Cited by 1,202
“The antagonistic activity of L. rhamnosus GG against enteropathogenic bacteria has also been shown in vivo in S. typhimurium infected mice (Hudault et al., 1997).”
BackgroundPotential Uses of Probiotics in Clinical Practice
Reid, G., Jaß, J., Sebulsky, M. T., & McCormick, J. K. (2003) · Clinical Microbiology Reviews · doi:10.1128/cmr.16.4.658-672.2003 · Cited by 918
“There is good in vitro evidence that certain probiotic strains can inhibit the growth and adhesion of a range of enteropathogens (12, 24, 25, 48, 59).”
BackgroundOverview of gut flora and probiotics
Holzapfel, W. H., Haberer, P., Snel, J., Schillinger, U., & Veld, J. H. J. H. I. (1998) · International Journal of Food Microbiology · doi:10.1016/s0168-1605(98)00044-0 · Cited by 884
Jacobsen, C. N., Nielsen, V. R., Hayford, A. E., Møller, P. L., Michaelsen, K. F., Pærregaard, A., Sandström, B., Tvede, M., & Jakobsen, M. (1999) · Applied and Environmental Microbiology · doi:10.1128/aem.65.11.4949-4956.1999 · Cited by 880
“Recently, Caco-2 cells have been used to examine the antimicrobial activity of lactobacilli (6, 13, 27) and bifidobacteria (5) against pathogenic bacteria.”
BackgroundMethod
Metadata
- Authors
- Hudault, S., Liévin, V, Bernet-Camard, M. F., & Servin, A. L.
- Affiliations
- Inserm (FR)
- CJF 94.07 INSERM, UPR de Pharmacie Paris XI, Chatenay-Malabry, France.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-02
- Volume
- 63
- Issue
- 2
- Pages
- 513-518
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC168342
- OpenAlex
- W2168298293
- Cited by
- 329
- References
- 37
- Citations by year
- 2026: 1 · 2024: 5 · 2023: 6 · 2022: 9 · 2021: 5 · 2020: 10 · 2019: 9 · 2018: 13 · 2017: 7 · 2016: 19 · 2015: 8 · 2014: 13 · 2013: 10 · 2012: 14
- FWCI
- 30.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Salmonella and Campylobacter epidemiology
- Life Sciences › Immunology and Microbiology › Microbiology › Reproductive tract infections research
- Keywords
- Salmonella typhimurium, probiotics, antagonistic activity, Caco-2 cells, germ-free mice, intestinal colonization, bacterial translocation, mesenteric lymph nodes, gastrointestinal infections
- MeSH terms
- Animals, Antibiosis, Culture Media, Feces, Female, Germ-Free Life, Humans, Intestines, Lacticaseibacillus casei, Lymph Nodes, Mesentery, Mice, Inbred C3H, Salmonella Infections, Animal, Salmonella typhimurium, Caco-2 Cells, Mice
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
