Cited by 17Probiotics and Fermented FoodsBacterial Genetics and BiotechnologyEnzyme Production and Characterization

The effects of adding lactococcal proteinase on the growth rate of Lactococcus lactis in milk depend on the type of enzyme

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Helinck, S, Richard, J, Juillard, V·1997·Applied and Environmental Microbiology

Abstract

Increasing the proteolytic activity of Lactococcus lactis cultures in milk by adding the corresponding proteinase resulted in a stimulation of the growth rate regardless of the strain and the type of proteinase, demonstrating that the rate of casein degradation was responsible for the growth rate limitation of L. lactis in milk. However, the stimulation was only transient, and the reduction in growth rate in the poststimulation phase depended on the type of cell envelope proteinase. When a PI-type proteinase was added, three causes were involved in the subsequent reduction in growth rate: degradation of the added proteinase, repression of the proteolytic activity expressed by the cells, and competition for peptide uptake. When a PIII-type proteinase was added, the cessation of stimulation was due to the autoproteolysis of the added enzyme only.

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Registry

Crossref · 10.1128/aem.63.6.2124-2130.1997

Cites

35 of 35 references verified0 citation errors · 0 not checkable

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Authors
Helinck, S., Richard, J., & Juillard, V
Affiliations
  • Unité de Recherches Laitières, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
  • Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
Venue
Applied and Environmental Microbiology
Published
1997-06
Volume
63
Issue
6
Pages
2124-2130
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2103515472
Cited by
17
References
35
Citations by year
2019: 1
FWCI
3.97
Type
journal article
Language
en
Open access
Locations
  • Applied and Environmental Microbiology (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
  • HAL (submittedVersion)
Topics
  • Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
Keywords
Lactococcus lactis, casein degradation, proteolytic activity, cell envelope proteinase, autoproteolysis, dairy fermentation
MeSH terms
Animals, Caseins, Cell Division, Cell Membrane, Kinetics, Milk, Endopeptidases, Lactococcus lactis

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