The effects of adding lactococcal proteinase on the growth rate of Lactococcus lactis in milk depend on the type of enzyme
Open accessAbstract
Increasing the proteolytic activity of Lactococcus lactis cultures in milk by adding the corresponding proteinase resulted in a stimulation of the growth rate regardless of the strain and the type of proteinase, demonstrating that the rate of casein degradation was responsible for the growth rate limitation of L. lactis in milk. However, the stimulation was only transient, and the reduction in growth rate in the poststimulation phase depended on the type of cell envelope proteinase. When a PI-type proteinase was added, three causes were involved in the subsequent reduction in growth rate: degradation of the added proteinase, repression of the proteolytic activity expressed by the cells, and competition for peptide uptake. When a PIII-type proteinase was added, the cessation of stimulation was due to the autoproteolysis of the added enzyme only.
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Positive role of cell wall anchored proteinase PrtP in adhesion of lactococci
Habimana, O., Le Goff, C., Juillard, V., Bellon-Fontaine, M.-N., Buist, G., Kulakauskas, S., & Briandet, R. (2007) · BMC Microbiology · doi:10.1186/1471-2180-7-36 · Cited by 58 · see citing works
“Proteolytic activity of cloned PrtP proteinases used in this work is comparable to that of a wild type strain, suggesting that their expression and anchoring could be also comparable [35].”
MethodSelf-citation
- Authors
- Helinck, S., Richard, J., & Juillard, V
- Affiliations
- Unité de Recherches Laitières, Institut National de la Recherche Agronomique, Jouy-en-Josas, France.
- Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement (FR)
- ORCID
- Juillard, V
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 1997-06
- Volume
- 63
- Issue
- 6
- Pages
- 2124-2130
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- PMC
- PMC168501
- OpenAlex
- W2103515472
- Cited by
- 17
- References
- 35
- Citations by year
- 2019: 1
- FWCI
- 3.97
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Applied and Environmental Microbiology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- HAL (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Biotechnology › Enzyme Production and Characterization
- Keywords
- Lactococcus lactis, casein degradation, proteolytic activity, cell envelope proteinase, autoproteolysis, dairy fermentation
- MeSH terms
- Animals, Caseins, Cell Division, Cell Membrane, Kinetics, Milk, Endopeptidases, Lactococcus lactis
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