The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.
Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli
Open accessAbstract
Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.
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Bradford, M. M. (1976) · Analytical Biochemistry · doi:10.1016/0003-2697(76)90527-3 · Cited by 255,302
Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
Altschul, S. (1997) · Nucleic Acids Research · doi:10.1093/nar/25.17.3389 · Cited by 73,302
A comprehensive set of sequence analysis programs for the VAX
Devereux, J., Haeberli, P., & Smithies, O. (1984) · Nucleic Acids Research · doi:10.1093/nar/12.1part1.387 · Cited by 14,222
Schägger, H., & von Jagow, G. (1987) · Analytical Biochemistry · doi:10.1016/0003-2697(87)90587-2 · Cited by 11,444
Phylogenetic identification and in situ detection of individual microbial cells without cultivation
Amann, R., Ludwig, W., & SCHLEIFER, K. H. (1995) · Microbiological Reviews · doi:10.1128/mmbr.59.1.143-169.1995 · Cited by 9,093
The PROSITE database, its status in 1995
Bairoch, A. (1996) · Nucleic Acids Research · doi:10.1093/nar/24.1.189 · Cited by 1,924
Winkler, U. K., & Stuckmann, M. (1979) · Journal of Bacteriology · doi:10.1128/jb.138.3.663-670.1979 · Cited by 1,116
The PROSITE database, its status in 1999
Hofmann, K., Bucher, P., Falquet, L., & Bairoch, A. (1999) · Nucleic Acids Research · doi:10.1093/nar/27.1.215 · Cited by 1,092
‘Interfacial activation’ of lipases: facts and artifacts
Verger, R. (1997) · Trends in Biotechnology · doi:10.1016/s0167-7799(96)10064-0 · Cited by 880
Jaeger, K. (1994) · FEMS Microbiology Reviews · doi:10.1016/0168-6445(94)90025-6 · Cited by 840
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R., G., Q., B., & P., L. (2002) · Applied Microbiology and Biotechnology · doi:10.1007/s00253-002-0975-y · Cited by 1,603
Daniel, R. (2005) · Nature Reviews Microbiology · doi:10.1038/nrmicro1160 · Cited by 862
Self-citationMetagenomic Analyses: Past and Future Trends
Simon, C., & Daniel, R. (2011) · Applied and Environmental Microbiology · doi:10.1128/aem.02345-10 · Cited by 731
Self-citationUncultured soil bacteria are a reservoir of new antibiotic resistance genes
Riesenfeld, C. S., Goodman, R. M., & Handelsman, J. (2004) · Environmental Microbiology · doi:10.1111/j.1462-2920.2004.00664.x · Cited by 527
Opportunities to improve fiber degradation in the rumen: microbiology, ecology, and genomics
Krause, D. O., Denman, S. E., Mackie, R. I., Morrison, M., Rae, A. L., Attwood, G. T., & McSweeney, C. S. (2003) · FEMS Microbiology Reviews · doi:10.1016/s0168-6445(03)00072-x · Cited by 512
Metadata
- Authors
- Henne, A., Schmitz, R. A., Bömeke, M., Gottschalk, G., & Daniel, R.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2000-07
- Volume
- 66
- Issue
- 7
- Pages
- 3113-3116
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W1978737414
- Cited by
- 302
- References
- 18
- FWCI
- 9.07
- Type
- journal article
- Language
- en
- Open access
- green
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- Henne, A., Schmitz, R. A., Bömeke, M., Gottschalk, G., & Daniel, R. (2000). Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.66.7.3113-3116.2000
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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