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Cited by 302

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli

Open access
Henne, Anke, Schmitz, Ruth A., Bömeke, Mechthild, Gottschalk, Gerhard, Daniel, Rolf·2000·Applied and Environmental Microbiology

Abstract

Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.

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Crossref · 10.1128/aem.66.7.3113-3116.2000

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Metadata

Authors
Henne, A., Schmitz, R. A., Bömeke, M., Gottschalk, G., & Daniel, R.
Venue
Applied and Environmental Microbiology
Published
2000-07
Volume
66
Issue
7
Pages
3113-3116
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W1978737414
Cited by
302
References
18
FWCI
9.07
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli. https://app.cochat.ai/papers/10.1128/aem.66.7.3113-3116.2000
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Henne, A., Schmitz, R. A., Bömeke, M., Gottschalk, G., & Daniel, R. (2000). Screening of Environmental DNA Libraries for the Presence of Genes Conferring Lipolytic Activity on Escherichia coli. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.66.7.3113-3116.2000

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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).

Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.