The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Uncultured soil bacteria are a reservoir of new antibiotic resistance genes
Abstract
Summary: Antibiotic resistance genes are typically isolated by cloning from cultured bacteria or by polymerase chain reaction (PCR) amplification from environmental samples. These methods do not access the potential reservoir of undiscovered antibiotic resistance genes harboured by soil bacteria because most soil bacteria are not cultured readily, and PCR detection of antibiotic resistance genes depends on primers that are based on known genes. To explore this reservoir, we isolated DNA directly from soil samples, cloned the DNA and selected for clones that expressed antibiotic resistance in Escherichia coli . We constructed four libraries that collectively contain 4.1 gigabases of cloned soil DNA. From these and two previously reported libraries, we identified nine clones expressing resistance to aminoglycoside antibiotics and one expressing tetracycline resistance. Based on the predicted amino acid sequences of the resistance genes, the resistance mechanisms include efflux of tetracycline and inactivation of aminoglycoside antibiotics by phosphorylation and acetylation. With one exception, all the sequences are considerably different from previously reported sequences. The results indicate that soil bacteria are a reservoir of antibiotic resistance genes with greater genetic diversity than previously accounted for, and that the diversity can be surveyed by a culture‐independent method.
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Citations
Cites
Prokaryotes: The unseen majority
Whitman, W. B., Coleman, D. C., & Wiebe, W. J. (1998) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.95.12.6578 · Cited by 4,871 · cited 1×
“…cultured on standard laboratory media (Giovannoni et al ., 1990; Ward et al ., 1990; Amann et al ., 1995; Suzuki et al ., 1997; Hugenholtz et al ., 1998), and the diversity of the uncultured majority is vast (Head et al ., 1998; Torsvik et al ., 1998; Whitman et al ., 1998; Béjà et al ., 2002).”
Background
Cited by
Origins and evolution of antibiotic resistance
Davies, J., & Davies, D. (2010) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00016-10 · Cited by 5,784
Call of the wild: antibiotic resistance genes in natural environments
Allen, H. K., Donato, J., Wang, H. H., Cloud-Hansen, K. A., Davies, J., & Handelsman, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2312 · Cited by 2,361
Self-citationManyi-Loh, C., Mamphweli, S., Meyer, E., & Okoh, A. (2018) · Molecules · doi:10.3390/molecules23040795 · Cited by 1,808
Dissemination of Antimicrobial Resistance in Microbial Ecosystems through Horizontal Gene Transfer
von Wintersdorff, C. J. H., Penders, J., van Niekerk, J. M., Mills, N. D., Majumder, S., van Alphen, L. B., Savelkoul, P. H. M., & Wolffs, P. F. G. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00173 · Cited by 1,707
Sampling the Antibiotic Resistome
D'Costa, V. M., McGrann, K. M., Hughes, D. W., & Wright, G. D. (2006) · Science · doi:10.1126/science.1120800 · Cited by 1,594
Metadata
- Authors
- Riesenfeld, C. S., Goodman, R. M., & Handelsman, J.
- Venue
- Environmental Microbiology
- Publisher
- Wiley
- Published
- 2004-08-11
- Volume
- 6
- Issue
- 9
- Pages
- 981-989
- ISSN
- 1462-2912 (ISSN-L)
- 1462-2920
- OpenAlex
- W2165524029
- Cited by
- 527
- References
- 1
- FWCI
- 8.45
- Type
- journal article
- Language
- en
- Open access
- closed
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- Riesenfeld, C. S., Goodman, R. M., & Handelsman, J. (2004). Uncultured soil bacteria are a reservoir of new antibiotic resistance genes. Environmental Microbiology. https://doi.org/10.1111/j.1462-2920.2004.00664.x
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