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Cited by 339

The DOI matches its Crossref record; 3 of the 3 references it cites were found in a registry or an index.

gfp -Based N -Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication

Open access
Andersen, Jens Bo, Heydorn, Arne, Hentzer, Morten, Eberl, Leo, Geisenberger, Otto, Christensen, Bjarke Bak, Molin, So/ren, Givskov, Michael·2001·Applied and Environmental Microbiology

Abstract

In order to perform single-cell analysis and online studies of N -acyl homoserine lactone (AHL)-mediated communication among bacteria, components of the Vibrio fischeri quorum sensor encoded by luxR-P luxI have been fused to modified versions of gfp mut3∗ genes encoding unstable green fluorescent proteins. Bacterial strains harboring this green fluorescent sensor detected a broad spectrum of AHL molecules and were capable of sensing the presence of 5 nM N -3-oxohexanoyl- l -homoserine lactone in the surroundings. In combination with epifluorescent microscopy, the sensitivity of the sensor enabled AHL detection at the single-cell level and allowed for real-time measurements of fluctuations in AHL concentrations. This green fluorescent AHL sensor provides a state-of-the-art tool for studies of communication between the individuals present in mixed bacterial communities.

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Crossref · 10.1128/aem.67.2.575-585.2001

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Metadata

Authors
Andersen, J. B., Heydorn, A., Hentzer, M., Eberl, L., Geisenberger, O., Christensen, B. B., Molin, S., & Givskov, M.
Venue
Applied and Environmental Microbiology
Published
2001-02
Volume
67
Issue
2
Pages
575-585
ISSN
  • 0099-2240 (ISSN-L)
  • 1098-5336
OpenAlex
W2085222365
Cited by
339
References
3
FWCI
5.54
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: gfp -Based N -Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication. https://app.cochat.ai/papers/10.1128/aem.67.2.575-585.2001
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Andersen, J. B., Heydorn, A., Hentzer, M., Eberl, L., Geisenberger, O., Christensen, B. B., Molin, S., & Givskov, M. (2001). gfp -Based N -Acyl Homoserine-Lactone Sensor Systems for Detection of Bacterial Communication. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.67.2.575-585.2001

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