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Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR
Open accessAbstract
To reduce PCR bias derived from a primer mismatch, the effect of the annealing temperature on the product ratio was investigated by denaturing gradient gel electrophoresis analysis of PCR products from a mixture of perfect-match and one-mismatch templates. These templates were generated by PCR from Pediococcus acidilactici for one mismatch and Micrococcus luteus for the perfect match. PCRs showed that the bias was reduced at lower temperatures. An environmental sample was also examined.
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Citations
Cited by
Takahashi, S., Tomita, J., Nishioka, K., Hisada, T., & Nishijima, M. (2014) · PLoS ONE · doi:10.1371/journal.pone.0105592 · Cited by 1,506
Systematic improvement of amplicon marker gene methods for increased accuracy in microbiome studies
Gohl, D. M., Vangay, P., Garbe, J., MacLean, A., Hauge, A., Becker, A., Gould, T. J., Clayton, J. B., Johnson, T. J., Hunter, R., et al. (2016) · Nature Biotechnology · doi:10.1038/nbt.3601 · Cited by 819
Acinas, S. G., Sarma-Rupavtarm, R., Klepac-Ceraj, V., & Polz, M. F. (2005) · Applied and Environmental Microbiology · doi:10.1128/aem.71.12.8966-8969.2005 · Cited by 625
Bias and artifacts in multitemplate polymerase chain reactions (PCR)
Kanagawa, T. (2003) · Journal of Bioscience and Bioengineering · doi:10.1016/s1389-1723(03)90130-7 · Cited by 566
Sipos, R., Székely, A. J., Palatinszky, M., Révész, S., Márialigeti, K., & Nikolausz, M. (2007) · FEMS Microbiology Ecology · doi:10.1111/j.1574-6941.2007.00283.x · Cited by 461
Metadata
- Authors
- Ishii, K., & Fukui, M.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2001-08
- Volume
- 67
- Issue
- 8
- Pages
- 3753-3755
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2142686163
- Cited by
- 225
- References
- 0
- FWCI
- 1.95
- Type
- journal article
- Language
- en
- Open access
- green
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- Ishii, K., & Fukui, M. (2001). Optimization of Annealing Temperature To Reduce Bias Caused by a Primer Mismatch in Multitemplate PCR. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.67.8.3753-3755.2001
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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