The DOI matches its Crossref record; 1 of the 1 references it cites were found in a registry or an index.
Scanning Transmission X-Ray, Laser Scanning, and Transmission Electron Microscopy Mapping of the Exopolymeric Matrix of Microbial Biofilms
Open accessAbstract
Confocal laser scanning microscopy (CLSM), transmission electron microscopy (TEM), and soft X-ray scanning transmission X-ray microscopy (STXM) were used to map the distribution of macromolecular subcomponents (e.g., polysaccharides, proteins, lipids, and nucleic acids) of biofilm cells and matrix. The biofilms were developed from river water supplemented with methanol, and although they comprised a complex microbial community, the biofilms were dominated by heterotrophic bacteria. TEM provided the highest-resolution structural imaging, CLSM provided detailed compositional information when used in conjunction with molecular probes, and STXM provided compositional mapping of macromolecule distributions without the addition of probes. By examining exactly the same region of a sample with combinations of these techniques (STXM with CLSM and STXM with TEM), we demonstrate that this combination of multimicroscopy analysis can be used to create a detailed correlative map of biofilm structure and composition. We are using these correlative techniques to improve our understanding of the biochemical basis for biofilm organization and to assist studies intended to investigate and optimize biofilms for environmental remediation applications.
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Cites
Infection of Plants and Plant Tissue Cultures with Cyanobacteria–Bacteria Complexes
Lobakova, E. S., Shchelmanova, A. G., Korzhenevskaya, T. G., & Gusev, M. V. (2001) · Microbiology · doi:10.1023/a:1010451210773 · Cited by 19
Cited by
Microbial Polysaccharides Template Assembly of Nanocrystal Fibers
Chan, C. S., De Stasio, G., Welch, S. A., Girasole, M., Frazer, B. H., Nesterova, M. V., Fakra, S., & Banfield, J. F. (2004) · Science · doi:10.1126/science.1092098 · Cited by 423
Viscoelasticity of biofilms and their recalcitrance to mechanical and chemical challenges
Peterson, B. W., He, Y., Ren, Y., Zerdoum, A., Libera, M. R., Sharma, P. K., van Winkelhoff, A.-J., Neut, D., Stoodley, P., van der Mei, H. C., et al. (2015) · FEMS Microbiology Reviews · doi:10.1093/femsre/fuu008 · Cited by 346
Dammak, L., Fouilloux, J., Bdiri, M., Larchet, C., Renard, E., Baklouti, L., Sarapulova, V., Kozmai, A., & Pismenskaya, N. (2021) · Membranes · doi:10.3390/membranes11100789 · Cited by 93
McCutcheon, J., & Southam, G. (2018) · Chemical Geology · doi:10.1016/j.chemgeo.2018.09.016 · Cited by 70
“The challenge of maintaining the structure of extracellular polymeric substances (EPS) (Lawrence et al., 2003) and the microbe-mineral relationships that exist in hydrated biofilms has been the focus of several studies, which have primarily turned to cryogenic ACCEPTED MANUSCRIPT”
BackgroundA Technological Understanding of Biofilm Detection Techniques: A Review
Achinas, S., Yska, S. K., Charalampogiannis, N., Krooneman, J., & Euverink, G. J. W. (2020) · Materials · doi:10.3390/ma13143147 · Cited by 46
Metadata
- Authors
- Lawrence, J. R., Swerhone, G. D. W., Leppard, G. G., Araki, T., Zhang, X., West, M. M., & Hitchcock, A. P.
- Venue
- Applied and Environmental Microbiology
- Publisher
- American Society for Microbiology
- Published
- 2003-09
- Volume
- 69
- Issue
- 9
- Pages
- 5543-5554
- ISSN
- 0099-2240 (ISSN-L)
- 1098-5336
- OpenAlex
- W2143303915
- Cited by
- 366
- References
- 1
- FWCI
- 4.93
- Type
- journal article
- Language
- en
- Open access
- green
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- Lawrence, J. R., Swerhone, G. D. W., Leppard, G. G., Araki, T., Zhang, X., West, M. M., & Hitchcock, A. P. (2003). Scanning Transmission X-Ray, Laser Scanning, and Transmission Electron Microscopy Mapping of the Exopolymeric Matrix of Microbial Biofilms. Applied and Environmental Microbiology. https://doi.org/10.1128/aem.69.9.5543-5554.2003
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
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