Cited by 964Molecular Biology Techniques and ApplicationsBiosensors and Analytical DetectionBacterial Identification and Susceptibility Testing

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Multiplex PCR: Optimization and Application in Diagnostic Virology

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Elnifro, Elfath M., Ashshi, Ahmed M., Cooper, Robert J., Klapper, Paul E.·2000·Clinical Microbiology Reviews

DOI 10.1128/cmr.13.4.559-570.2000PMC PMC88949

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Abstract

SUMMARY: PCR has revolutionized the field of infectious disease diagnosis. To overcome the inherent disadvantage of cost and to improve the diagnostic capacity of the test, multiplex PCR, a variant of the test in which more than one target sequence is amplified using more than one pair of primers, has been developed. Multiplex PCRs to detect viral, bacterial, and/or other infectious agents in one reaction tube have been described. Early studies highlighted the obstacles that can jeopardize the production of sensitive and specific multiplex assays, but more recent studies have provided systematic protocols and technical improvements for simple test design. The most useful of these are the empirical choice of oligonucleotide primers and the use of hot start-based PCR methodology. These advances along with others to enhance sensitivity and specificity and to facilitate automation have resulted in the appearance of numerous publications regarding the application of multiplex PCR in the diagnosis of infectious agents, especially those which target viral nucleic acids. This article reviews the principles, optimization, and application of multiplex PCR for the detection of viruses of clinical and epidemiological importance.

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Avoiding false positives with PCR
Kwok, S., & Higuchi, R. (1989) · Nature · doi:10.1038/339237a0 · Cited by 3,407
Inhibition and facilitation of nucleic acid amplification
Wilson, I. G. (1997) · Applied and Environmental Microbiology · doi:10.1128/aem.63.10.3741-3751.1997 · Cited by 2,298
Bias caused by template annealing in the amplification of mixtures of 16S rRNA genes by PCR
Suzuki, M. T., & Giovannoni, S. (1996) · Applied and Environmental Microbiology · doi:10.1128/aem.62.2.625-630.1996 · Cited by 2,021
Bias in template-to-product ratios in multitemplate PCR
Polz, M. F., & Cavanaugh, C. M. (1998) · Applied and Environmental Microbiology · doi:10.1128/aem.64.10.3724-3730.1998 · Cited by 1,484
Deletion screening of the Duchenne muscular dystrophy locus via multiplex DNA amplification
Chamberlain, J. S., Gibbs, R. A., Rainer, J. E., Nguyen, P. N., & Caskey, C. T. (1988) · Nucleic Acids Research · doi:10.1093/nar/16.23.11141 · Cited by 1,271
Multiplex PCR: Critical Parameters and Step-by-Step Protocol
Henegariu, O., Heerema, N. A., Dlouhy, S. R., Vance, G. H., & Vogt, P. H. (1997) · BioTechniques · doi:10.2144/97233rr01 · Cited by 956
Prevention of pre-PCR mis-priming and primer dimerization improves low-copy-number amplifications
Chou, Q., Russell, M., Birch, D. E., Raymond, J., & Bloch, W. (1992) · Nucleic Acids Research · doi:10.1093/nar/20.7.1717 · Cited by 742
General concepts for PCR primer design.Preprint
Dieffenbach, C. W., Lowe, T. M., & Dveksler, G. S. (1993) · Genome Research · doi:10.1101/gr.3.3.s30 · Cited by 486
Multiplex PCR: advantages, development, and applications.Preprint
Edwards, M. C., & Gibbs, R. A. (1994) · Genome Research · doi:10.1101/gr.3.4.s65 · Cited by 417
Microchip Device for Cell Lysis, Multiplex PCR Amplification, and Electrophoretic Sizing
Waters, L. C., Jacobson, S. C., Kroutchinina, N., et al. (1998) · Analytical Chemistry · doi:10.1021/ac970642d · Cited by 405

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Authors: Elnifro, E. M., Ashshi, A. M., Cooper, R. J., & Klapper, P. E.
Affiliations: University of Manchester (GB)
School of Medicine, The University of Manchester,1 and
ORCID: Klapper, Paul E.
Venue: Clinical Microbiology Reviews
Publisher: American Society for Microbiology
Published: 2000-10
Volume: 13
Issue: 4
Pages: 559-570
ISSN: 0893-8512 (ISSN-L)
1098-6618
DOI: 10.1128/cmr.13.4.559-570.2000
PMC: PMC88949
OpenAlex: W2137089963
Cited by: 964
References: 126
Citations by year: 2026: 54 · 2025: 58 · 2024: 67 · 2023: 60 · 2022: 50 · 2021: 57 · 2020: 51 · 2019: 38 · 2018: 29 · 2017: 37 · 2016: 22 · 2015: 43 · 2014: 30 · 2013: 46 · 2012: 40
FWCI: 0.55
Type: journal article
Language: en
Open access: green
europepmc.org
Licence: journals.asm.org/non-commercial-tdm-license (tdm)
Locations: Clinical Microbiology Reviews (publishedVersion)
Europe PMC (PubMed Central) (publishedVersion)
PubMed Central (publishedVersion)
PubMed (publishedVersion)
Research Explorer (The University of Manchester) (publishedVersion)
Topics: Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Molecular Biology Techniques and Applications
Physical Sciences › Engineering › Biomedical Engineering › Biosensors and Analytical Detection
Health Sciences › Medicine › Microbiology (medical) › Bacterial Identification and Susceptibility Testing
Keywords: multiplex PCR, PCR optimization, diagnostic virology, virus detection, primer design, hot-start PCR, infectious disease diagnosis, viral nucleic acids, assay sensitivity and specificity
MeSH terms: Humans, Virology, Virus Diseases, Viruses, Polymerase Chain Reaction

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Multiplex PCR: Optimization and Application in Diagnostic Virology

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