Complete Sequence of Probiotic Symbioflor 2 Escherichia coli Strain G3/10 and Draft Sequences of Symbioflor 2 E. coli Strains G1/2, G4/9, G5, G6/7, and G8
Open accessAbstract
The complete genome of probiotic Escherichia coli strain G3/10 is presented here. In addition, the probiotic E. coli strains G1/2, G4/9, G5, G6/7, and G8 are presented in draft form. These six strains together comprise the probiotic product Symbioflor 2 (DSM 17252).
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E. coli DSM 17252 is devoid of potential pathogenic genes.
“As regards the E. coli mixture described before (DSM 17252), it is devoid of potential pathogenic genes, but the information about the gene pks is not precisely reported [49].”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [49]
The Genome Announcement identifies DSM 17252 as the six-strain Symbioflor 2 product and describes it as safe based on clinical use and risk-group classification. It does not state that the strains lack potential pathogenic genes; searches for pathogenic or virulence genes and pks found no such assessment in our copy. No page number is available for these passages. (LLM, conf=0.73)
Experimental — accuracy not yet measuredInformation about the pks gene in E. coli DSM 17252 is not precisely reported.
“As regards the E. coli mixture described before (DSM 17252), it is devoid of potential pathogenic genes, but the information about the gene pks is not precisely reported [49].”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [49]
The Genome Announcement identifies the six strains in DSM 17252 and describes genome sequencing and annotation, but it does not discuss the pks gene. Searches for pks, colibactin, and pathogenic or virulence genes found no passage assessing how precisely pks information was reported. Under the evidence rule, that absence alone does not verify the claim. (LLM, conf=0.72)
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“G1/2 (DSM 16441) 5,090,326 252 JPKH00000000 pSYM10 (1,549), pSYM12 (7,111) KM107846 (pSYM10), KM107848 (pSYM12) G3/10 (DSM 16443) 4,999,267 12 JPKI00000000 pSYM1 (50,572), pSYM2 (4,197), pSYM3 (1,934), pSYM4 (1,304), pSYM5 (6,567), pSYM6 (10,433) JN887338 (pSYM1), KM107838 (pSYM2), KM107839 (pSYM3), KM107840 (pSYM4),…”
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“As regards the E. coli mixture described before (DSM 17252), it is devoid of potential pathogenic genes, but the information about the gene pks is not precisely reported [49].”
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- Authors
- Zschüttig, A., Auerbach, C., Meltke, S., Eichhorn, C., Brandt, M., Blom, J., Goesmann, A., Jarek, M., Scharfe, M., Zimmermann, K., Wassenaar, T. M., & Gunzer, F.
- Affiliations
- Technische Universität Dresden (DE)
- Justus-Liebig-Universität Gießen (DE)
- Helmholtz Centre for Infection Research (DE)
- SymbioPharm (Germany) (DE)
- Molecular Microbiology and Genomics Consultants (Germany) (DE)
- Corresponding author
- Wassenaar, Trudy M.
- Gunzer, Florian
- Venue
- Genome Announcements
- Publisher
- American Society for Microbiology
- Published
- 2015-04-30
- Volume
- 3
- Issue
- 2
- ISSN
- 2169-8287 (ISSN-L)
- PMC
- PMC4358396
- OpenAlex
- W1969116438
- Cited by
- 16
- References
- 8
- Citations by year
- 2025: 1 · 2024: 2 · 2022: 1 · 2020: 1 · 2019: 3 · 2018: 2 · 2017: 2 · 2016: 3 · 2015: 1
- FWCI
- 3.57
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Genome Announcements (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- HZI (Helmholtz Centre for Infection Research) (submittedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- probiotic Escherichia coli, complete genome sequence, draft genome sequence, probiotic strains, bacterial genomics
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