Complete genome sequences of five Escherichia coli strains with probiotic attributes
Open accessAbstract
The complete genome sequences of five Escherichia coli strains with probiotic attributes were determined, including strain A0 34/86, a component of the probiotic product Colinfant New Born, and strains H22, 582, B771, and B1172 with published probiotic potential. The size of sequenced genomes ranged from 5,092 to 5,408 kb.
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E. coli A0 34/86 is apparently devoid of the pks gene.
“Noteworthy, E. coli A0 34/86, a strain apparently devoid of the pks gene [47], contains at least two potential pathogenic genes (hly, cnf-1) respectively responsible for alpha-hemolysin and cytotoxic necrotizing factor-1 production and release [48].”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [47]
The Announcement reports sequencing E. coli A0 34/86, and Table 1 gives its genome characteristics, but neither states whether it carries the pks gene. Searches for “pks gene” and “colibactin polyketide synthase” found no passage addressing its presence or absence. (LLM, conf=0.76)
Experimental — accuracy not yet measuredIn germ-free mice administered Nissle 1917, intestinal epithelial cells showed evidence of DNA damage, which was not observed with clbA or clbP mutants.
“In germ-free mice administered with Nissle 1917, intestinal epithelial cells showed evidence of DNA damage [132], which was not observed when mice were inoculated with variants of Nissle 1917 carrying mutations in clbA or clbP”
From Selection, Comparative Genomics, and Potential Probiotic Features of Escherichia coli 5C, a pks-Negative Strain Isolated from Healthy Infant Donor Feces (2026) · cites this paper as [47]
The ANNOUNCEMENT describes sequencing five E. coli strains, and its genome-characteristics table lists strains other than Nissle 1917 (page not provided). Searches for germ-free mice, intestinal epithelial DNA damage, Nissle 1917, clbA, and clbP found no passage addressing the claimed experiment. (LLM, conf=0.76)
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Cites
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Hrala, M., Bosák, J., Micenková, L., Křenová, J., Lexa, M., Pirková, V., Tomáštíková, Z., Koláčková, I., & Šmajs, D. (2021) · Applied and Environmental Microbiology · doi:10.1128/aem.03121-20 · Cited by 23 · see citing works
Self-citationMicenková, L., Bosák, J., Smatana, S., Novotný, A., Budínská, E., & Šmajs, D. (2019) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-019-09548-3 · Cited by 11 · see citing works
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Di Pierro, F., Sagheddu, V., Galletti, S., Casaroli, A., Labrini, E., Soldi, S., Cazzaniga, M., Bertuccioli, A., Matera, M., Cavecchia, I., et al. (2026) · Probiotics and Antimicrobial Proteins · doi:10.1007/s12602-025-10522-5 · Cited by 5 · see citing works
“…Nissle 1917, intestinal epithelial cells showed evidence of DNA damage [132], which was not observed when mice were inoculated with variants of Nissle 1917 carrying mutations in clbA or clbP , genes encoded within the pks cluster and which are directly involved in colibactin synthesis [47, 133].”
- Authors
- Fedrová, P., Hrala, M., Tom, N., Micenková, L., Nascimento, A. M. A., Bosák, J., & Šmajs, D.
- Affiliations
- Masaryk University (CZ)
- Universidade Federal de Minas Gerais (BR)
- Corresponding author
- Šmajs, David
- Venue
- Microbiology Resource Announcements
- Publisher
- American Society for Microbiology
- Published
- 2023-09-19
- Volume
- 12
- Issue
- 9
- Pages
- e0036323-e0036323
- ISSN
- 2576-098X (ISSN-L)
- PMC
- PMC10508162
- OpenAlex
- W4385620930
- Cited by
- 1
- References
- 14
- Citations by year
- 2025: 1
- FWCI
- 0.14
- Type
- journal article
- Language
- en
- Open access
- diamond
- doi.org
- Licence
- Locations
- Microbiology Resource Announcements (publishedVersion, cc-by)
- PubMed Central (publishedVersion, cc-by)
- PubMed (publishedVersion)
- Funders
- European Union - Programme EXCELES (ID Project No. LX22NPO5103)
- Operational Programme Research, Development, and Education (CZ.02.2.69/0.0/0.0/19_073/0016943)
- e-INFRA CZ (90140)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Escherichia coli, probiotic strains, complete genome sequence, genome size
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