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Aerobactin, but Not Yersiniabactin, Salmochelin, or Enterobactin, Enables the Growth/Survival of Hypervirulent (Hypermucoviscous) Klebsiella pneumoniae Ex Vivo and In Vivo
Open accessAbstract
The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence factor in systemic infection. However, strains of hvKP commonly produce the additional siderophores yersiniabactin, salmochelin, and enterobactin. The roles of these siderophores in hvKP infection have not been optimally defined. To that end, site-specific gene disruptions were created in hvKP1 (wild type), resulting in the generation of hvKP1Δ iucA (aerobactin deficient), hvKP1Δ iroB (salmochelin deficient), hvKP1Δ entB (enterobactin and salmochelin deficient), hvKP1Δ irp2 (yersiniabactin deficient), and hvKP1Δ entB Δ irp2 (enterobactin, salmochelin, and yersiniabactin deficient). The growth/survival of these constructs was compared to that of their wild-type parent hvKP1 ex vivo in human ascites fluid, human serum, and human urine and in vivo in mouse systemic infection and pulmonary challenge models. Interestingly, in contrast to aerobactin, the inability to produce enterobactin, salmochelin, or yersiniabactin individually or in combination did not decrease the ex vivo growth/survival in human ascites or serum or decrease virulence in the in vivo infection models. Surprisingly, none of the siderophores increased growth in human urine. In human ascites fluid supplemented with exogenous siderophores, siderophores increased the growth of hvKP1Δ iucA , with the relative activity being enterobactin > aerobactin > yersiniabactin > salmochelin, suggesting that the contribution of aerobactin to virulence is dependent on both innate biologic activity and quantity produced. Taken together, these data confirm and extend a role for aerobactin as a critical virulence factor for hvKP. Since it appears that aerobactin production is a defining trait of hvKP strains, this factor is a potential antivirulence target.
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“The elaboration and acquisition of the latter three siderophores are associated with the pathogenesis and hypervirulence of K. pneumoniae (1,44,53,54).”
From Siderophore-mediated iron acquisition by Klebsiella pneumoniae (2024) · cites this paper as [54]
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“However, unlike FeEnt, FeAbn uptake was not inhibited by SCN, reinforcing its importance in the pathogenesis of hvKp1 (54).”
From Siderophore-mediated iron acquisition by Klebsiella pneumoniae (2024) · cites this paper as [54]
Experimental — accuracy not yet measuredIn the citing paper
“The glucosylated ferric catecholate does not adsorb well to siderocalin ( Kumar et al., 2022 ), which promotes the pathogenesis of clinical isolates of E. coli, K. pneumoniae, and Salmonella typhimurium in human and animal hosts ( Russo et al., 2011 , 2015 ).”
From Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu (2024) · cites this paper as Russo et al., 2015
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Cites
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Hypervirulent (hypermucoviscous) Klebsiella pneumoniae
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Sorted by citations
Klebsiella pneumoniae: Going on the Offense with a Strong Defense
Paczosa, M. K., & Mecsas, J. C. (2016) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00078-15 · Cited by 2,090
“mouse infections that serve as a model for liver abscesses but not in a pneumonic model (319, 331).”
BackgroundHypervirulent Klebsiella pneumoniae
Russo, T. A., & Marr, C. M. (2019) · Clinical Microbiology Reviews · doi:10.1128/cmr.00001-19 · Cited by 1,398
“that enhances virulence ex vivo and in vivo (87).”
BackgroundInfluentialSelf-citationPopulation genomics of Klebsiella pneumoniae
Wyres, K. L., Lam, M. M. C., & Holt, K. E. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0315-1 · Cited by 1,175
Lam, M. M. C., Wick, R. R., Watts, S. C., Cerdeira, L., Wyres, K. L., & Holt, K. E. (2021) · Nature Communications · doi:10.1038/s41467-021-24448-3 · Cited by 1,093
Colonization, Infection, and the Accessory Genome of Klebsiella pneumoniae
Martin, R. M., & Bachman, M. A. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00004 · Cited by 1,067
“Furthermore, the siderophore Aer has been distinguished as the most common siderophore secreted by hypervirulent K. pneumoniae (Russo et al., 2015).”
Background
Metadata
- Authors
- Russo, T. A., Olson, R., MacDonald, U., Beanan, J., & Davidson, B. A.
- Affiliations
- VA Western New York Healthcare System (US)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2015-08
- Volume
- 83
- Issue
- 8
- Pages
- 3325-3333
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC4496593
- OpenAlex
- W2283434174
- Semantic Scholar
- e716de1372bc2153e94e76130028b8b30a86c6b0
- Cited by
- 305
- References
- 49
- Citations by year
- 2026: 32 · 2025: 32 · 2024: 34 · 2023: 33 · 2022: 56 · 2021: 28 · 2020: 25 · 2019: 16 · 2018: 24 · 2017: 11 · 2016: 13 · 2015: 1
- FWCI
- 12.66
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Veterans Affairs
- National Institutes of Health (W81XWH)
- National Institute of Allergy and Infectious Diseases (R21AI088318)
- Telemedicine and Advanced Technology Research Center
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Immunology and Microbiology › Microbiology › Enterobacteriaceae and Cronobacter Research
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Keywords
- aerobactin, yersiniabactin, salmochelin, enterobactin, siderophores, hypervirulent Klebsiella pneumoniae, hypermucoviscous Klebsiella pneumoniae, virulence factors, iron acquisition, iucA, IRP2, entB, iroB, systemic infection, pulmonary infection, ascitic fluid, human serum, human urine
- MeSH terms
- Animals, Enterobactin, Glucosides, Humans, Hydroxamic Acids, Klebsiella Infections, Klebsiella pneumoniae, Male, Phenols, Thiazoles, Virulence, Siderophores, Microbial Viability, Mice, Young Adult
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