The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Aerobactin, but Not Yersiniabactin, Salmochelin, or Enterobactin, Enables the Growth/Survival of Hypervirulent (Hypermucoviscous) Klebsiella pneumoniae Ex Vivo and In Vivo
Open accessAbstract
The siderophore aerobactin is the dominant siderophore produced by hypervirulent Klebsiella pneumoniae (hvKP) and was previously shown to be a major virulence factor in systemic infection. However, strains of hvKP commonly produce the additional siderophores yersiniabactin, salmochelin, and enterobactin. The roles of these siderophores in hvKP infection have not been optimally defined. To that end, site-specific gene disruptions were created in hvKP1 (wild type), resulting in the generation of hvKP1Δ iucA (aerobactin deficient), hvKP1Δ iroB (salmochelin deficient), hvKP1Δ entB (enterobactin and salmochelin deficient), hvKP1Δ irp2 (yersiniabactin deficient), and hvKP1Δ entB Δ irp2 (enterobactin, salmochelin, and yersiniabactin deficient). The growth/survival of these constructs was compared to that of their wild-type parent hvKP1 ex vivo in human ascites fluid, human serum, and human urine and in vivo in mouse systemic infection and pulmonary challenge models. Interestingly, in contrast to aerobactin, the inability to produce enterobactin, salmochelin, or yersiniabactin individually or in combination did not decrease the ex vivo growth/survival in human ascites or serum or decrease virulence in the in vivo infection models. Surprisingly, none of the siderophores increased growth in human urine. In human ascites fluid supplemented with exogenous siderophores, siderophores increased the growth of hvKP1Δ iucA , with the relative activity being enterobactin > aerobactin > yersiniabactin > salmochelin, suggesting that the contribution of aerobactin to virulence is dependent on both innate biologic activity and quantity produced. Taken together, these data confirm and extend a role for aerobactin as a critical virulence factor for hvKP. Since it appears that aerobactin production is a defining trait of hvKP strains, this factor is a potential antivirulence target.
Overview
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Integrity signals
Registry
Cites
Statistics
Verification
Claims the paper makes
The claims and statistics of this paper haven’t been checked yet.
Run the check — free, no account needed. It reads the open-access text and checks the sources it cites.
Claims other papers make about this one
Each is checked against this paper’s own text: does it say what the citing paper says it does?
The cited source doesn’t support the claim as written.
In the citing paper
“The elaboration and acquisition of the latter three siderophores are associated with the pathogenesis and hypervirulence of K. pneumoniae (1,44,53,54).”
From Siderophore-mediated iron acquisition by Klebsiella pneumoniae (2024) · cites this paper as [54]
Checked by a reasoning model — may be wrong.
Experimental — accuracy not yet measuredThe source backs part of the claim, not all of it — or backs it for a different population, condition or comparison.
In the citing paper
“However, unlike FeEnt, FeAbn uptake was not inhibited by SCN, reinforcing its importance in the pathogenesis of hvKp1 (54).”
From Siderophore-mediated iron acquisition by Klebsiella pneumoniae (2024) · cites this paper as [54]
Experimental — accuracy not yet measuredIn the citing paper
“The glucosylated ferric catecholate does not adsorb well to siderocalin ( Kumar et al., 2022 ), which promotes the pathogenesis of clinical isolates of E. coli, K. pneumoniae, and Salmonella typhimurium in human and animal hosts ( Russo et al., 2011 , 2015 ).”
From Specificity and mechanism of TonB-dependent ferric catecholate uptake by Fiu (2024) · cites this paper as Russo et al., 2015
Self-check
Percentages, sums and reported differences re-computed from the paper’s own numbers.
The self-check needs the paper’s full text.
Citations
Cites
Guide for the Care and Use of Laboratory Animals: Eighth Edition
(2011) · doi:10.17226/12910 · Cited by 16,199
Lipocalin 2 mediates an innate immune response to bacterial infection by sequestrating iron
Flo, T. H., Smith, K. D., Sato, S., Rodriguez, D. J., Holmes, M. A., Strong, R. K., Akira, S., & Aderem, A. (2004) · Nature · doi:10.1038/nature03104 · Cited by 1,877
Siderophore-Based Iron Acquisition and Pathogen Control
Miethke, M., & Marahiel, M. A. (2007) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00012-07 · Cited by 1,774
Chemistry and biology of siderophores
Hider, R. C., & Kong, X. (2010) · Natural Product Reports · doi:10.1039/b906679a · Cited by 1,722
Guide for the Care and Use of Laboratory Animals (8th edn)
Albus, U. (2012) · Laboratory Animals · doi:10.1258/la.2012.150312 · Cited by 1,706
Weinberg, E. D. (1978) · Microbiological Reviews · doi:10.1128/mmbr.42.1.45-66.1978 · Cited by 1,254
Neilands, J. B. (1981) · Annual Review of Biochemistry · doi:10.1146/annurev.bi.50.070181.003435 · Cited by 1,232
Hypervirulent (hypermucoviscous) Klebsiella pneumoniae
Shon, A. S. Y., Bajwa, R. P. S., & Russo, T. A. (2013) · Virulence · doi:10.4161/viru.22718 · Cited by 1,217
Self-citation(2018) · doi:10.1001/jama · Cited by 932
Snitkin, E. S., Zelazny, A. M., Thomas, P. J., Stock, F., Program, N. C. S., Henderson, D. K., Palmore, T. N., & Segre, J. A. (2012) · Science Translational Medicine · doi:10.1126/scitranslmed.3004129 · Cited by 863
Research works printed but not linked: 1
- 10.1128/mr.42.1.45-66.1978 · DOI · Paper page
Cited by
Sorted by citations
Klebsiella pneumoniae: Going on the Offense with a Strong Defense
Paczosa, M. K., & Mecsas, J. C. (2016) · Microbiology and Molecular Biology Reviews · doi:10.1128/mmbr.00078-15 · Cited by 2,090
“mouse infections that serve as a model for liver abscesses but not in a pneumonic model (319, 331).”
BackgroundHypervirulent Klebsiella pneumoniae
Russo, T. A., & Marr, C. M. (2019) · Clinical Microbiology Reviews · doi:10.1128/cmr.00001-19 · Cited by 1,398
“that enhances virulence ex vivo and in vivo (87).”
BackgroundInfluentialSelf-citationPopulation genomics of Klebsiella pneumoniae
Wyres, K. L., Lam, M. M. C., & Holt, K. E. (2020) · Nature Reviews Microbiology · doi:10.1038/s41579-019-0315-1 · Cited by 1,175
Lam, M. M. C., Wick, R. R., Watts, S. C., Cerdeira, L., Wyres, K. L., & Holt, K. E. (2021) · Nature Communications · doi:10.1038/s41467-021-24448-3 · Cited by 1,093
Colonization, Infection, and the Accessory Genome of Klebsiella pneumoniae
Martin, R. M., & Bachman, M. A. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00004 · Cited by 1,067
“Furthermore, the siderophore Aer has been distinguished as the most common siderophore secreted by hypervirulent K. pneumoniae (Russo et al., 2015).”
BackgroundRusso, T. A., Olson, R., Fang, C.-T., Stoesser, N., Miller, M., MacDonald, U., Hutson, A., Barker, J. H., La Hoz, R. M., Johnson, J. R., et al. (2018) · Journal of Clinical Microbiology · doi:10.1128/jcm.00776-18 · Cited by 783
“The murine sepsis model was as described previously (26, 33).”
BackgroundMethodSelf-citationHypervirulent Klebsiella pneumoniae – clinical and molecular perspectives
Choby, J. E., Howard-Anderson, J. R., & Weiss, D. S. (2019) · Journal of Internal Medicine · doi:10.1111/joim.13007 · Cited by 747
Klebsiella pneumoniae: an increasing threat to public health
Effah, C. Y., Sun, T., Liu, S., & Wu, Y. (2020) · Annals of Clinical Microbiology and Antimicrobials · doi:10.1186/s12941-019-0343-8 · Cited by 558
Lee, C.-R., Lee, J. H., Park, K. S., Jeon, J. H., Kim, Y. B., Cha, C., Jeong, B. C., & Lee, S. H. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00483 · Cited by 496
“…yersiniabactin, plays a crucial role in the growth and survival of hvKP in human ascites fluid or serum and in the in vivo mouse infection models (Russo et al., 2015), suggesting that aerobactin is a crucial virulence factor of hvKP. Aerobactin is encoded by the iucABCD operon and its cognate…”
BackgroundHypervirulence and hypermucoviscosity: Two different but complementary Klebsiella spp. phenotypes?
Catalán-Nájera, J. C., Garza–Ramos, U., & Barrios-Camacho, H. (2017) · Virulence · doi:10.1080/21505594.2017.1317412 · Cited by 364
“tion of a mouse pulmonary challenge model was also identified.(66) It is noteworthy that these effects were not”
BackgroundWyres, K. L., Nguyen, T. N. T., Lam, M. M. C., Judd, L. M., Châu, N. V. V., Dance, D. A. B., Ip, M., Karkey, A., Ling, C. L., Miliya, T., et al. (2020) · Genome Medicine · doi:10.1186/s13073-019-0706-y · Cited by 324
Zhang, Y., Zhao, C., Wang, Q., Wang, X., Chen, H., Li, H., Zhang, F., Li, S., Wang, R., & Wang, H. (2016) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01127-16 · Cited by 333
“Recently, aerobactin was considered a defining genetic trait and potential antivirulence target for hvKP (24).”
BackgroundVirulence Factors in Hypervirulent Klebsiella pneumoniae
Zhu, J., Wang, T., Chen, L., & Du, H. (2021) · Frontiers in Microbiology · doi:10.3389/fmicb.2021.642484 · Cited by 349
Lan, P., Jiang, Y., Zhou, J., & Yu, Y. (2021) · Journal of Global Antimicrobial Resistance · doi:10.1016/j.jgar.2021.02.020 · Cited by 282
Lam, M. M. C., Wyres, K. L., Judd, L. M., Wick, R. R., Jenney, A. W. J., Brisse, S., & Holt, K. E. (2018) · Genome Medicine · doi:10.1186/s13073-018-0587-5 · Cited by 277
Abbas, R., Chakkour, M., Dine, H. Z. E., Obaseki, E., Obeid, S. T., Jezzini, A., Ghssein, G., & Ezzeddine, Z. (2024) · Biology · doi:10.3390/biology13020078 · Cited by 227
“Aerobactin (Iuc), on the other hand, has been long known to be associated with virulence; numerous studies have shown that it plays a critical role in enhanced iron acquisition, bacterial growth, and/or virulence in a variety of murine models, human ascites fluid, and blood [18].”
BackgroundCarroll, C. S., & Moore, M. M. (2018) · Critical Reviews in Biochemistry and Molecular Biology · doi:10.1080/10409238.2018.1476449 · Cited by 181
“Because K. pneumoniae secretes yersiniabactin, salmochelin, and enterobactin, Russo et al. (2015) created gene knockout strains for each siderophore (DentB was deficient in enterobactin and salmochelin, Dirp2 was deficient in yersiniabactin and DentBirp2 was deficient for yersiniabactin,…”
BackgroundLiu, C., & Guo, J. X. (2019) · Annals of Clinical Microbiology and Antimicrobials · doi:10.1186/s12941-018-0302-9 · Cited by 165
Klebba, P. E., Newton, S. M. C., Six, D. A., Kumar, A., Yang, T., Nairn, B. L., Munger, C., & Chakravorty, S. (2021) · Chemical Reviews · doi:10.1021/acs.chemrev.0c01005 · Cited by 150
The emerging problems ofKlebsiella pneumoniaeinfections: carbapenem resistance and biofilm formation
Chung, P. Y. (2016) · FEMS Microbiology Letters · doi:10.1093/femsle/fnw219 · Cited by 156
Liu, C., Du, P., Xiao, N., Ji, F., Russo, T. A., & Guo, J. X. (2020) · Virulence · doi:10.1080/21505594.2020.1809322 · Cited by 151
Self-citationComplement resistance mechanisms of Klebsiella pneumoniae
Doorduijn, D. J., Rooijakkers, S. H. M., van Schaik, W., & Bardoel, B. W. (2016) · Immunobiology · doi:10.1016/j.imbio.2016.06.014 · Cited by 149
Li, J., Ren, J., Wang, W., Wang, G., Gu, G., Wu, X., Wang, Y., Huang, M., & Li, J. (2017) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-017-3160-z · Cited by 138
Kochan, T. J., Nozick, S., Valdes, A., Mitra, S. D., Cheung, B. H., Lebrun-Corbin, M., Medernach, R. L., Vessely, M., Mills, J. O., Axline, C. M. R., et al. (2023) · Nature Communications · doi:10.1038/s41467-023-43802-1 · Cited by 145
Gomez‐Simmonds, A., & Uhlemann, A. (2017) · The Journal of Infectious Diseases · doi:10.1093/infdis/jiw378 · Cited by 130
“invasive infection in in vitro and mouse models [80].”
BackgroundGomes, A. É. I., Stuchi, L. P., Siqueira, N. M. G., Henrique, J. B., Vicentini, R., Ribeiro, M. L., Darrieux, M., & Ferraz, L. F. C. (2018) · Scientific Reports · doi:10.1038/s41598-018-27420-2 · Cited by 127
Bulger, J. L., MacDonald, U., Olson, R., Beanan, J. M., & Russo, T. A. (2017) · Infection and Immunity · doi:10.1128/iai.00093-17 · Cited by 125
“establish the importance of aerobactin as a virulence factor for hvKP (24).”
BackgroundInfluentialSelf-citationZhang, S., Zhang, X., Wu, Q. F., Zheng, X., Dong, G., Fang, R., Zhang, Y., Cao, J., & Zhou, T. (2019) · Antimicrobial Resistance and Infection Control · doi:10.1186/s13756-019-0615-2 · Cited by 122
Hu, Y., Anes, J., Devineau, S., & Fanning, S. (2020) · Foodborne Pathogens and Disease · doi:10.1089/fpd.2020.2847 · Cited by 118
“…presence of these clusters on virulence plasmids, which are often co-located with resistance markers such as the rmpA and kfuABC or the two-component regulatory system kvgAS (Nassif and Sansonetti, 1986; Darfeuille-Michaud et al. , 1992; Chen et al. , 2004; Wu et al. , 2009; Russo et al. , 2015).”
Shankar, C., Veeraraghavan, B., Nabarro, L., Ravi, R., Ragupathi, N. K. D., & Rupali, P. (2018) · BMC Microbiology · doi:10.1186/s12866-017-1148-6 · Cited by 111
“Aerobactin enables the survival of hvKp in vivo and ex vivo and plays a significant role than the other siderophores such as enterobactin and yersiniabactin [27].”
BackgroundHan, Y., Wen, X., Zhao, W., Cao, X., Wen, J.-X., Wang, J., Hu, Z.-D., & Zheng, W. (2022) · Frontiers in Microbiology · doi:10.3389/fmicb.2022.1003783 · Cited by 111
Yan, Q., Zhou, M., Zou, M., & Liu, W.-E. (2016) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-015-2551-2 · Cited by 107
“This has generated great interest of developing antivirulence agents to target the virulence factors of hvKP strains instead of using antimicrobial to kill them [14, 20].”
BackgroundMethodInfluentialThe making of hypervirulent Klebsiella pneumoniae
Dai, P., & Hu, D. (2022) · Journal of Clinical Laboratory Analysis · doi:10.1002/jcla.24743 · Cited by 111
Hypervirulent Klebsiella pneumoniae: a Call for Consensus Definition and International Collaboration
Harada, S., & Doi, Y. (2018) · Journal of Clinical Microbiology · doi:10.1128/jcm.00959-18 · Cited by 100
“Among them, the iuc genes appear to be critical in conferring accentuated virulence (14, 15).”
BackgroundThe Basics of Bacteriuria: Strategies of Microbes for Persistence in Urine
Ipe, D. S., Horton, E. M., & Ulett, G. C. (2016) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2016.00014 · Cited by 98
Lu, M., Chen, Y.-T., Chiang, M., Wang, Y., Hsiao, P.-Y., Huang, Y.-J., Lin, C.-T., Cheng, C., Liang, C.-L., & Lai, Y. (2017) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2017.00103 · Cited by 101
“Salmochelin, enterobactin, and yersiniabactin are dispensable for the growth and survival of hypervirulent K. pneumoniae strains ex vivo and in vivo (Russo et al., 2015).”
Kochan, T. J., Nozick, S., Medernach, R. L., Cheung, B. H., Gatesy, S. W. M., Lebrun-Corbin, M., Mitra, S. D., Khalatyan, N., Krapp, F., Qi, C., et al. (2022) · BMC Infectious Diseases · doi:10.1186/s12879-022-07558-1 · Cited by 93
“A 2015 report suggested that aerobactin was the only siderophore required for the hypervirulence phenotype of the ST86K2 capsule type strain hvKP1 in both mouse intraperitoneal and pneumonia models [51].”
BackgroundTowards new antibiotics classes targeting bacterial metallophores
Ezzeddine, Z., & Ghssein, G. (2023) · Microbial Pathogenesis · doi:10.1016/j.micpath.2023.106221 · Cited by 92
Lin, Z., Zheng, J., Bai, B., Xu, G., Lin, F.-J., Chen, Z., Sun, X., Qu, D., Yu, Z., & Deng, Q.-W. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00436 · Cited by 84
Liu, Z., Gu, Y., Li, X., Liu, Y., Ye, Y., Guan, S., & Li, J. (2018) · Annals of Laboratory Medicine · doi:10.3343/alm.2019.39.2.167 · Cited by 83
Virulence factors in carbapenem-resistant hypervirulent Klebsiella pneumoniae
Mendes, G., Santos, M. L., Ramalho, J. F., Duarte, A., & Caneiras, C. S. G. (2023) · Frontiers in Microbiology · doi:10.3389/fmicb.2023.1325077 · Cited by 88
Liu, C., Shi, J., & Guo, J. X. (2018) · Infection and Drug Resistance · doi:10.2147/idr.s161075 · Cited by 84
“Until recently, aerobactin, the dominant siderophore, was regarded as a critical virulence factor for hvKp genetic background.(1,8,9) A multicenter study, focusing on middle-aged patients, first demonstrated the clinical and molecular characteristic of hvKp (defined as aerobactin positive) infection.”
BackgroundAnatomy of an extensively drug-resistant Klebsiella pneumoniae outbreak in Tuscany, Italy
Martin, M. J., Corey, B. W., Sannio, F., Hall, L. R., MacDonald, U., Jones, B. T., Mills, E. G., Harless, C., Stam, J. A., Maybank, R. A., et al. (2021) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.2110227118 · Cited by 78
Self-citationRusso, T. A., MacDonald, U., Hassan, S., Camanzo, E., Lebreton, F., Corey, B. W., & McGann, P. (2021) · mSphere · doi:10.1128/msphere.00045-21 · Cited by 76
Self-citationNonribosomal peptide synthetase biosynthetic clusters of ESKAPE pathogens
Gulick, A. M. (2017) · Natural Product Reports · doi:10.1039/c7np00029d · Cited by 73
Straková, N., Kořená, K., & Karpíšková, R. (2021) · Toxicon · doi:10.1016/j.toxicon.2021.04.007 · Cited by 79
Shankar, C., Basu, S., Lal, B., Shanmugam, S., Vasudevan, K., Mathur, P., Ramaiah, S., Anbarasu, A., & Veeraraghavan, B. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2021.709681 · Cited by 73
High Serum Iron level is Associated with Increased Mortality in Patients with Sepsis
Lan, P., Pan, K.-H., Wang, S., Shi, Q., Yu, Y., Fu, Y., Chen, Y., Jiang, Y., Hua, X., Zhou, J., et al. (2018) · Scientific Reports · doi:10.1038/s41598-018-29353-2 · Cited by 78
Joseph, L., Merciecca, T., Forestier, C., Balestrino, D., & Miquel, S. (2021) · Microorganisms · doi:10.3390/microorganisms9061282 · Cited by 82
Zhang, J., Xu, Y., Wang, M., Li, X., Liu, Z., Kuang, D., Deng, Z., Ou, H., & Qu, J. (2023) · Genome Medicine · doi:10.1186/s13073-023-01260-w · Cited by 69
“The virulence genes include the iuc cluster, rmpADC cluster, and rmpA2 , which could confer hypervirulence to K. pneumoniae [16, 32–34].”
BackgroundResultLiu, C., & Guo, J. X. (2018) · Antimicrobial Resistance and Infection Control · doi:10.1186/s13756-018-0371-8 · Cited by 70
Bailey, D. C., Alexander, E. M., Rice, M. R., Drake, E. J., Mydy, L. S., Aldrich, C. C., & Gulick, A. M. (2018) · Journal of Biological Chemistry · doi:10.1074/jbc.ra118.002798 · Cited by 75
“However, in vitro and in vivo experiments have both demonstrated that of the four siderophores, knocking out the production of only aerobactin significantly diminished the virulence of hvKP (26, 27).”
BackgroundShaidullina, E., Schwabe, M., Rohde, T. D., Shapovalova, V. V., Dyachkova, M. S., Дмитриевна, М. А., Savochkina, Y. A., Shelenkov, A. A., Mikhaylova, Y., Sydow, K., et al. (2023) · Genome Medicine · doi:10.1186/s13073-023-01159-6 · Cited by 65
“The presence of certain biomarkers, for example, peg-344 (metabolite transporter), iucA (aerobactin), iroB (salmochelin), and rmpA (regulator hypermucoviscosity), has been shown to predict hypervirulence phenotypes and distinguish hvKp from non-hvKp strains [6, 7].”
BackgroundAn overview of recent progress in siderophore-antibiotic conjugates
Kong, H., Cheng, W., Han, W., Yuan, Y., Yang, Z., & Zhang, X. (2019) · European Journal of Medicinal Chemistry · doi:10.1016/j.ejmech.2019.111615 · Cited by 62
Potential therapeutic targets of Klebsiella pneumoniae: a multi-omics review perspective
Ali, S., Alam, M., Hasan, G. M., & Hassan, M. I. (2021) · Briefings in Functional Genomics · doi:10.1093/bfgp/elab038 · Cited by 62
Whole‐genome analysis of Klebsiella pneumoniae from bovine mastitis milk in the U.S.
Zheng, Z., Gorden, P. J., Xia, X.-Q., Zheng, Y., & Li, G. (2021) · Environmental Microbiology · doi:10.1111/1462-2920.15721 · Cited by 59
Global Evolution of Pathogenic Bacteria With Extensive Use of Fluoroquinolone Agents
Füzi, M., Rodríguez‐Baño, J., & Tóth, Á. (2020) · Frontiers in Microbiology · doi:10.3389/fmicb.2020.00271 · Cited by 57
Fu, L., Huang, M., Zhang, X., Yang, X., Liu, Y., Zhang, L., Zhang, Z., Wang, G., & Zhou, Y. (2018) · Microbial Pathogenesis · doi:10.1016/j.micpath.2018.01.030 · Cited by 56
Liu, X., Xu, Q., Yang, X., Heng, H., Yang, C., Yang, G., Peng, M., Chan, E. W., & Chen, S. (2025) · mBio · doi:10.1128/mbio.03838-24 · Cited by 52
Hetta, H. F., Alanazi, F. E., Ali, M. A. S., Alatawi, A. D., Aljohani, H. M., Ahmed, R., Alansari, N. A., Alkhathami, F., Albogmi, A. A., Alharbi, B. M., et al. (2025) · Pharmaceuticals · doi:10.3390/ph18050724 · Cited by 62
“This concealment may limit the O1 antigen’s interaction with host immune components, raising questions about its functional role in the context of hvKP pathogenesis [119,120].”
Marcoleta, A. E., Berríos-Pastén, C., Núñez, G., Monasterio, O., & Lagos, R. (2016) · Frontiers in Microbiology · doi:10.3389/fmicb.2016.00849 · Cited by 50
New putative vaccine candidates against Acinetobacter baumannii using the reverse vaccinology method
Fereshteh, S., Abdoli, S., Shahcheraghi, F., Ajdary, S., Nazari, M., & Badmasti, F. (2020) · Microbial Pathogenesis · doi:10.1016/j.micpath.2020.104114 · Cited by 51
Wang, X., Xie, Y., Li, G., Liu, J., Li, X., Tian, L., Sun, J., Ou, H., & Qu, H. (2018) · Virulence · doi:10.1080/21505594.2017.1421894 · Cited by 50
“Aerobactin showed relatively higher biological activity than yersiniabactin or salmochelin [47].”
BackgroundTargeting human pathogenic bacteria by siderophores: A proteomics review
Ferreira, D., Seca, A. M. L., Diana, C. G. A., & Silva, A. M. S. (2016) · Journal of Proteomics · doi:10.1016/j.jprot.2016.04.006 · Cited by 49
Chen, T., Ying, L., Xiong, L., Wang, X., Lu, P., Wang, Y., Shen, P., & Xiao, Y. (2024) · hLife · doi:10.1016/j.hlife.2024.06.005 · Cited by 47
“Aerobactin accounts for > 90% of total siderophore activity, and only aerobactin signi fi cantly enhances survival in human ascites, serum, and outbred mouse systemic and pulmonary infection models [70,74].”
A Serendipitous Mutation Reveals the Severe Virulence Defect of a Klebsiella pneumoniae fepB Mutant
Palacios, M., Broberg, C. A., Walker, K. A., & Miller, V. L. (2017) · mSphere · doi:10.1128/msphere.00341-17 · Cited by 50
Xu, C., Dong, N., Chen, K., Yang, X., Zeng, P., Hou, C., Chan, E. W., Yao, X., & Chen, S. (2022) · iScience · doi:10.1016/j.isci.2022.103894 · Cited by 47
“The chemically diverse siderophores produced by hvKP included aerobactin, yersiniabactin, salmochelin, and enterobactin, the activity of which was reported to be six- to 10-fold higher than that of cKP (Russo et al., 2015).”
BackgroundRusso, T. A., MacDonald, U., Drayer, Z. J., Davies, C. J., Alvarado, C. L., Hutson, A. D., Luo, T. L., Martin, M. J., McGann, P., & Lebreton, F. (2024) · EBioMedicine · doi:10.1016/j.ebiom.2024.105302 · Cited by 46
Self-citationValenzuela, M., González, I., & Palavecino, C. E. (2019) · Photodiagnosis and Photodynamic Therapy · doi:10.1016/j.pdpdt.2019.08.012 · Cited by 45
Virulence Factors in Klebsiella pneumoniae: A Literature Review
Monteiro, A. D. S. S., Cordeiro, S. M., & Pedreira, J. N. R. (2024) · Indian Journal of Microbiology · doi:10.1007/s12088-024-01247-0 · Cited by 49
“Aerobactin is a critical virulence factor often found in hyper-virulent K. pneumoniae strains but rare in classical strains [34, 42, 90, 95, 106, 107].”
Marcoleta, A. E., Varas, M. A., Ortíz-Severín, J., Vásquez, L., Berríos-Pastén, C., Sabag, A., Chávez, F. P., Allende, M. L., Santiviago, C. A., Monasterio, O., et al. (2018) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2018.00030 · Cited by 44
“Regarding salmochelin production, a previous work with hypervirulent K. pneumoniae showed that aerobactin but not yersiniabactin, salmochelin, or enterobactin, were required for virulence over in vivo infection models (Russo et al., 2015).”
BackgroundWeber, B. S., Jong, A. M. D., Guo, A. B. Y., Dharavath, S., French, S., Fiebig‐Comyn, A., Coombes, B. K., Magolan, J., & Brown, E. D. (2020) · Cell Reports · doi:10.1016/j.celrep.2020.107927 · Cited by 44
Innate Host Defense against Klebsiella pneumoniae and the Outlook for Development of Immunotherapies
Opoku‐Temeng, C., Małachowa, N., Kobayashi, S. D., & DeLeo, F. R. (2021) · Journal of Innate Immunity · doi:10.1159/000518679 · Cited by 45
Siderophore-mediated iron acquisition by Klebsiella pneumoniae
Kumar, A., Chakravorty, S., Yang, T., Russo, T. A., Newton, S. M., & Klebba, P. E. (2024) · Journal of Bacteriology · doi:10.1128/jb.00024-24 · Cited by 42
“The elaboration and acquisition of the latter three siderophores are associated with the pathogenesis and hypervirulence of K. pneumoniae (1, 44, 53, 54).”
BackgroundSelf-citationTian, D., Wang, W., Li, M., Chen, W., Zhou, Y., Huang, Y., Sheng, Z.-K., & Jiang, X. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2021.752011 · Cited by 42
“Aerobactin is a critical virulence factor for hvKp, while the additional siderophores are not major contributors to high siderophore levels (Russo et al., 2015).”
BackgroundMai, D., Wu, A., Li, R., Cai, D., Tong, H., Wang, N., & Tan, J. (2023) · BMC Microbiology · doi:10.1186/s12866-023-03124-0 · Cited by 41
“Such as iuc (siderophore aerobic actin biosynthesis gene) [13], iro (salmonella bio-synthesis gene) [14], peg-344 (a metabolic transporter of unknown function) [15], rmpA and rmpA2 (a regulator that promotes capsule production) [16].”
BackgroundClinical and laboratory insights into the threat of hypervirulent Klebsiella pneumoniae
Han, X., Yao, J., He, J., Liu, H., Jiang, Y., Zhao, D., Shi, Q., Zhou, J., Hu, H., Lan, P., et al. (2024) · International Journal of Antimicrobial Agents · doi:10.1016/j.ijantimicag.2024.107275 · Cited by 43
Getting hypervirulent Klebsiella pneumoniae on the radar screen
Sellick, J. A., & Russo, T. A. (2018) · Current Opinion in Infectious Diseases · doi:10.1097/qco.0000000000000464 · Cited by 40
Self-citationMolecular validation of clinical Pantoea isolates identified by MALDI-TOF
Soutar, C. D., & Stavrinides, J. (2019) · PLoS ONE · doi:10.1371/journal.pone.0224731 · Cited by 40
“Aerobactin is a known virulence factor and has been demonstrated to be essential for the virulence of hypervirulent, Klebsiella pneumoniae [58,59].”
BackgroundSedighi, P., Zarei, O., Karimi, K., Taheri, M., Karami, P., & Shokoohizadeh, L. (2020) · International Journal of Microbiology · doi:10.1155/2020/8894727 · Cited by 40
Dereeper, A., Gruel, G., Pot, M., Couvin, D., Barbier, E., Bastian, S., Bambou, J., Gelu‐Siméon, M., Ferdinand, S., Guyomard-Rabenirina, S., et al. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.01242-22 · Cited by 40
“nantly to hypervirulence in laboratory experiments and mouse models of disease, while the inactivation of other siderophore systems has minimal effects (35, 36).”
BackgroundAlharbi, M. T., Almuhayawi, M. S., Nagshabandi, M. K., Tarabulsi, M. K., Alruhaili, M. H., Gattan, H. S., Jaouni, S. K. A., Selim, S., Alanazi, A., Alruwaili, Y. S., et al. (2023) · Microorganisms · doi:10.3390/microorganisms11030661 · Cited by 38
“icuA Forward Reverse 5′-GCATAGGCGGATACGAACAT-3′ [10] 5′-CACAGGGCAATTGCTTACCT-3′”
The pathogenicity of rmpA or aerobactin-positive Klebsiella pneumoniae in infected mice
Li, G., Sun, S., Zhao, Z. Y., & Sun, Y. (2019) · Journal of International Medical Research · doi:10.1177/0300060519863544 · Cited by 38
Huang, Y., Li, J., Wang, Q., Tang, K., Cai, X., & Li, C. (2022) · Journal of Hospital Infection · doi:10.1016/j.jhin.2022.09.014 · Cited by 37
Royer, G., Clermont, O., Marin, J., Condamine, B., Dion, S., Blanquart, F., Galardini, M., & Denamur, É. (2023) · Nature Communications · doi:10.1038/s41467-023-39428-y · Cited by 38
“Aerobactin but not yersiniabactin or salmochelin enables survival of hypervirulent Klebsiella pneumoniae in mouse systemic infection 59 and the sit operon is required for systemic stages of infection in Salmonella Typhimurium 60 and Yersinia pestis 61 .”
BackgroundBailey, D. C., Drake, E. J., Grant, T. D., & Gulick, A. M. (2016) · Biochemistry · doi:10.1021/acs.biochem.6b00409 · Cited by 39
Aerobactin Synthesis Proteins as Antivirulence Targets in Hypervirulent Klebsiella pneumoniae
Russo, T. A., & Gulick, A. M. (2019) · ACS Infectious Diseases · doi:10.1021/acsinfecdis.9b00117 · Cited by 37
Self-citationInterplay between siderophores and colibactin genotoxin in Escherichia coli
Martín, P., Tronnet, S., Garcie, C., & Oswald, É. (2017) · IUBMB Life · doi:10.1002/iub.1612 · Cited by 38
“pneumoniae results in an attenuated virulence phenotype in a diversity of models of infection (13).”
BackgroundRadisic, V., Grevskott, D. H., Lunestad, B. T., Øvreås, L., & Marathe, N. P. (2022) · International Journal of Hygiene and Environmental Health · doi:10.1016/j.ijheh.2022.114075 · Cited by 33
Sherif, M. M., Palmieri, M., Mirande, C., El‐Mahallawy, H. A., Rashed, H. G., Reheem, F. A. E., Elmanakhly, A. R., Rabea, R. A., Aboulela, A., Saeed, L. Y., et al. (2021) · European Journal of Clinical Microbiology & Infectious Diseases · doi:10.1007/s10096-021-04177-7 · Cited by 36
Silvester, R., Madhavan, A., Kokkat, A., Parolla, A., Adarsh, B. M., Harikrishnan, M., & Abdulla, M. H. (2021) · The Science of The Total Environment · doi:10.1016/j.scitotenv.2021.149859 · Cited by 38
Wu, X., Shi, Q., Shen, S., Huang, C., & Wu, H. (2021) · Frontiers in Cellular and Infection Microbiology · doi:10.3389/fcimb.2021.688989 · Cited by 38
Virulence Factors and Carbapenem-Resistance Mechanisms in Hypervirulent Klebsiella Pneumoniae
Liao, Y., Gong, J., Yuan, X., Wang, X., Huang, Y., & Chen, X. (2024) · Infection and Drug Resistance · doi:10.2147/idr.s461903 · Cited by 34
“53 Disrupting the synthesis of aerobactin could thus reduce the growth or viability of hvKP in human ascites and serum, while disruption of enterobactin, yersiniabactin, and salmochelin synthesis cannot achieve the same effect, indicating that aerobactin is a key virulence factor in hvKP.”
BackgroundWang, T.-C., Lin, J., Chang, J.-C., Hiaso, Y.-W., Wang, C.-H., Chiu, S., Fung, C., Chang, F., & Siu, L.-K. K. (2021) · Gut Pathogens · doi:10.1186/s13099-021-00439-z · Cited by 34
“Aerobactin has been suggested to play an important role in virulence and is also a key biomarker of the enhancement of the growth and survival of HvKp [23, 31].”
BackgroundDu, P., Liu, C., Fan, S., Baker, S. G., & Guo, J. X. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.01929-21 · Cited by 35
Huang, N., Jia, H., Zhou, B., Zhou, C., Cao, J., Liao, W., Liu, S., Wang, L., Chen, L., Chen, L., et al. (2022) · Frontiers in Public Health · doi:10.3389/fpubh.2022.991306 · Cited by 36
“pneumoniae growth and virulence in the host via regulation of iron supply, which could serve as a potential anti-virulence target (34, 37), and our study found that up to 66.”
BackgroundTsui, C. K. M., Abid, F. B., Ismail, K. A., McElheny, C. L., Maslamani, M. A. R. S. A., Omrani, A. S., & Doi, Y. (2023) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.00030-23 · Cited by 34
“lator, encoded by rmpA, and aerobactin (12, 13).”
BackgroundYu, F., Lv, J., Niu, S., Du, H., Tang, Y., Bonomo, R. A., Kreiswirth, B. N., & Chen, L. (2018) · Antimicrobial Agents and Chemotherapy · doi:10.1128/aac.01031-18 · Cited by 32
Fluorescent sensors of siderophores produced by bacterial pathogens
Kumar, A., Yang, T., Chakravorty, S., Majumdar, A., Nairn, B. L., Six, D. A., Marcondes dos Santos, N., Price, S. L., Lawrenz, M. B., Actis, L. A., et al. (2022) · Journal of Biological Chemistry · doi:10.1016/j.jbc.2022.101651 · Cited by 28
“pneumoniae (HvKp1, HvKp2 (39)) in iron-deficient MOPS (85) and M9 (86) minimal media, removed the cells by centrifugation, and analyzed the supernatants with decoy sensors.”
BackgroundMethodInfluentialSelf-citationRolbiecki, D., Harnisz, M., Korzeniewska, E., Buta, M., Hubeny, J., & Zieliński, W. (2021) · International Journal of Hygiene and Environmental Health · doi:10.1016/j.ijheh.2021.113831 · Cited by 33
Metadata
- Authors
- Russo, T. A., Olson, R., MacDonald, U., Beanan, J., & Davidson, B. A.
- Affiliations
- VA Western New York Healthcare System (US)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 2015-08
- Volume
- 83
- Issue
- 8
- Pages
- 3325-3333
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC4496593
- OpenAlex
- W2283434174
- Semantic Scholar
- e716de1372bc2153e94e76130028b8b30a86c6b0
- Cited by
- 305
- References
- 49
- Citations by year
- 2026: 32 · 2025: 32 · 2024: 34 · 2023: 33 · 2022: 56 · 2021: 28 · 2020: 25 · 2019: 16 · 2018: 24 · 2017: 11 · 2016: 13 · 2015: 1
- FWCI
- 12.66
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Infection and Immunity (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- U.S. Department of Veterans Affairs
- National Institutes of Health (W81XWH)
- National Institute of Allergy and Infectious Diseases (R21AI088318)
- Telemedicine and Advanced Technology Research Center
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Life Sciences › Immunology and Microbiology › Microbiology › Enterobacteriaceae and Cronobacter Research
- Health Sciences › Medicine › Microbiology (medical) › Antibiotic Resistance in Bacteria
- Keywords
- aerobactin, yersiniabactin, salmochelin, enterobactin, siderophores, hypervirulent Klebsiella pneumoniae, hypermucoviscous Klebsiella pneumoniae, virulence factors, iron acquisition, iucA, IRP2, entB, iroB, systemic infection, pulmonary infection, ascitic fluid, human serum, human urine
- MeSH terms
- Animals, Enterobactin, Glucosides, Humans, Hydroxamic Acids, Klebsiella Infections, Klebsiella pneumoniae, Male, Phenols, Thiazoles, Virulence, Siderophores, Microbial Viability, Mice, Young Adult
About these checks
Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.
Statistics and arithmetic are recomputed, not judged.
Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.
What counts as a claim ›How we measure confidence ›How we check this ›
Are you an author, or do you see a problem with this page? Request a review
