Cited by 69Antimicrobial Resistance in StaphylococcusBacterial Genetics and BiotechnologyBacterial biofilms and quorum sensing

Identification of Point Mutations in Clinical Staphylococcus aureus Strains That Produce Small-Colony Variants Auxotrophic for Menadione

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Dean, Melissa A., Olsen, Randall J., Long, S. Wesley, Rosato, Adriana E., Musser, James M.·2014·Infection and Immunity

Abstract

Staphylococcus aureus small-colony variants (SCVs) are implicated in chronic and relapsing infections that are difficult to diagnose and treat. Despite many years of study, the underlying molecular mechanisms and virulence effect of the small-colony phenotype remain incompletely understood. We sequenced the genomes of five S. aureus SCV strains recovered from human patients and discovered previously unidentified nonsynonymous point mutations in three genes encoding proteins in the menadione biosynthesis pathway. Analysis of genetic revertants and complementation with wild-type alleles confirmed that these mutations caused the SCV phenotype and decreased virulence for mice.

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Crossref · 10.1128/iai.01487-13

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Authors
Dean, M. A., Olsen, R. J., Long, S. W., Rosato, A. E., & Musser, J. M.
Affiliations
Houston Methodist (US)
Venue
Infection and Immunity
Published
2014-04
Volume
82
Issue
4
Pages
1600-1605
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
OpenAlex
W2097252391
Cited by
69
References
38
Citations by year
2026: 3 · 2025: 8 · 2024: 9 · 2023: 3 · 2022: 4 · 2021: 7 · 2020: 2 · 2019: 8 · 2018: 3 · 2017: 6 · 2016: 7 · 2015: 7 · 2014: 2
FWCI
5.14
Type
journal article
Language
en
Open access
Locations
  • Infection and Immunity (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • Houston Methodist Hospital
  • National Institutes of Health
  • Houston Methodist Research Institute
  • National Institute of Allergy and Infectious Diseases (R01AI080688)
Topics
  • Health Sciences › Medicine › Infectious Diseases › Antimicrobial Resistance in Staphylococcus
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
Staphylococcus aureus, small colony variants, virulence, revertants, complementation, chronic infection, infection relapse
MeSH terms
Animals, Anti-Bacterial Agents, Disease Models, Animal, Gentamicins, Microbial Sensitivity Tests, Phenotype, Staphylococcal Infections, Staphylococcus aureus, Virulence, Genome, Bacterial, Point Mutation, Sequence Analysis, DNA, Polymorphism, Single Nucleotide, Vitamin K 3, Mice

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