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Cited by 438

The DOI matches its Crossref record; 18 of the 18 references it cites were found in a registry or an index.

Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes

Open access
Gaillard, J L, Berche, P, Sansonetti, P·1986·Infection and Immunity
DOI 10.1128/iai.52.1.50-55.1986
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Abstract

The role of hemolysin in the virulence of Listeria monocytogenes was studied by using transposon mutagenesis. The 26-kilobase conjugative transposon Tn1545, originally found in Streptococcus pneumoniae, was transferred to a hemolytic virulent strain of L. monocytogenes. The frequency of transfer was estimated to be about 10(-8) per recipient. This allowed us to isolate a nonhemolytic mutant which most likely harbors a single copy of Tn1545. Loss of hemolysin production was associated with loss of virulence. The 50% lethal dose of the mutant was assessed to about 10(9.6) bacteria per mouse after intravenous challenge. Nonhemolytic bacteria were unable to grow in host tissues and were rapidly eliminated from the spleen and liver of infected mice. Virulence was restored in hemolysin-producing revertant obtained by spontaneous loss of transposon Tn1545. These results strongly suggest that hemolysin is a major virulence factor implicated in the intracellular growth of L. monocytogenes.

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Metadata

Authors
Gaillard, J. L., Berche, P., & Sansonetti, P.
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1986-04
Volume
52
Issue
1
Pages
50-55
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.52.1.50-55.1986
OpenAlex
W2141486991
Cited by
438
References
18
FWCI
23.76
Type
journal article
Language
en
Open access
green

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CoChat. (2026). Citation check: Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. https://app.cochat.ai/papers/10.1128/iai.52.1.50-55.1986
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Gaillard, J. L., Berche, P., & Sansonetti, P. (1986). Transposon mutagenesis as a tool to study the role of hemolysin in the virulence of Listeria monocytogenes. Infection and Immunity. https://doi.org/10.1128/iai.52.1.50-55.1986

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