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Cited by 178Urinary Tract Infections ManagementEscherichia coli research studiesBacterial biofilms and quorum sensing

The DOI matches its Crossref record; 28 of the 28 references it cites were found in a registry or an index.

Construction of a urease-negative mutant of Proteus mirabilis: analysis of virulence in a mouse model of ascending urinary tract infection

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Jones, B D, Lockatell, C V, Johnson, D E, Warren, J W, Mobley, H L·1990·Infection and Immunity
DOI 10.1128/iai.58.4.1120-1123.1990PMC PMC258592
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Abstract

Proteus mirabilis, a urease-producing uropathogen, causes serious urinary tract infections in humans. To specifically evaluate the contribution of urease to virulence, a mutation was introduced into P. mirabilis HI4320 by homologous recombination. Virulence was assessed in the CBA mouse model of ascending urinary tract infection. Twenty mice each were challenged transurethrally with P. mirabilis HI4320 and its urease-negative derivative (1 x 10(9) to 2 x 10(9) CFU). At 48 h animals were sacrificed and the mean log10 CFU per milliliter of urine (parent, 6.23; mutant, 4.19; P = 0.0014) or per gram of bladder (parent, 6.29; mutant, 4.28; P = 0.0002), left kidney (parent, 4.11; mutant, 1.02; P = 0.00009), and right kidney (parent, 4.11; mutant, 2.43; P = 0.036) were all shown to be significantly different. These data demonstrate a role for urease as a critical virulence determinant for uropathogenic P. mirabilis.

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Crossref · 10.1128/iai.58.4.1120-1123.1990

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Metadata

Authors
Jones, B. D., Lockatell, C. V., Johnson, D. E., Warren, J. W., & Mobley, H. L.
Affiliations
University of Maryland, Baltimore (US)
ORCID
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1990-04
Volume
58
Issue
4
Pages
1120-1123
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.58.4.1120-1123.1990
PMC
PMC258592
OpenAlex
W1928804885
Cited by
178
References
28
Citations by year
2026: 2 · 2025: 3 · 2024: 6 · 2023: 3 · 2022: 3 · 2021: 10 · 2020: 4 · 2019: 7 · 2018: 5 · 2017: 3 · 2016: 12 · 2015: 6 · 2014: 11 · 2013: 7 · 2012: 4
FWCI
6.71
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Infection and Immunity (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Funders
  • National Institutes of Health (5p01ag004393-09)
  • National Institute on Aging (AG-04393)
  • National Institute of Allergy and Infectious Diseases (AI23328)
Topics
  • Health Sciences › Medicine › Urology › Urinary Tract Infections Management
  • Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
  • Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
Keywords
Proteus mirabilis, urease, virulence factors, ascending urinary tract infection, uropathogenic bacteria, mouse model, bladder colonization, kidney colonization
MeSH terms
Animals, Female, Mice, Inbred CBA, Mutation, Proteus mirabilis, Urease, Urinary Tract Infections, Virulence, Mice

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