CoChat
Sign Up
Cited by 65Complement system in diseasesBiomarkers in Disease MechanismsT-cell and B-cell Immunology

Pneumococcal polysaccharides complexed with C3d bind to human B lymphocytes via complement receptor type 2

Open access
Griffioen, A W, Rijkers, G T, Janssens-Korpela, P, Zegers, B J·1991·Infection and Immunity
DOI 10.1128/iai.59.5.1839-1845.1991PMC PMC257924
Open this paper in CoChat — free account

Abstract

The immunoregulatory function of the complement system has been the focus of many investigations. In particular, fragments of complement factor C3 have been shown to play a role in B-lymphocyte activation and proliferation, lymphokine production, and the generation of in vitro antibody production. Purified pneumococcal polysaccharides (PS) can induce direct activation of C3 via the alternative pathway. Using sera of C1q-deficient patients and healthy subjects, we demonstrated that C3d, a split product of C3 that is generated after degradation of iC3b, can be bound to PS antigens. The binding of C3d to PS can occur in the absence of specific antibodies. Subsequently, we showed that PS complexed with C3d can be recognized by complement receptor type 2 that is expressed on B cells. Treatment of B cells with a monoclonal antibody recognizing the C3d-binding site of complement receptor type 2 reduces the binding of PS-C3d to the cells. In addition, we showed that PS4 complexed with C3d exerted an increased immunogenicity compared with free PS4. Our results show that the complement system plays a role in the activation of PS-specific B cells, carrying membrane receptors for C3d. Consequently, the complement system plays a regulatory role in the antibody response to T-cell-independent type 2 antigens such as PS.

Integrity signals

Registry

Crossref · 10.1128/iai.59.5.1839-1845.1991

Cites

39 of 39 references verified0 citation errors · 0 not checkable

Statistics

Recomputing p-values needs the paper’s full text.

Claims the paper makes

The text we found for this paper looks like another document, so its claims and self-check weren’t run. The registry and citation checks still apply.

Self-check

Percentages, sums and reported differences re-computed from the paper’s own numbers.

The text we found for this paper looks like another document, so the self-check wasn’t run.

Cites

Flagged first, then by citations

Cited by

Sorted by citations

Related

Authors
Griffioen, A. W., Rijkers, G. T., Janssens-Korpela, P., & Zegers, B. J.
Affiliations
University Medical Center Utrecht (NL)
ORCID
Venue
Infection and Immunity
Publisher
American Society for Microbiology
Published
1991-05
Volume
59
Issue
5
Pages
1839-1845
ISSN
  • 0019-9567 (ISSN-L)
  • 1098-5522
DOI
10.1128/iai.59.5.1839-1845.1991
PMC
PMC257924
OpenAlex
W2153684695
Cited by
65
References
39
Citations by year
2023: 1 · 2022: 1 · 2021: 1 · 2018: 1 · 2016: 1 · 2015: 2 · 2014: 3 · 2013: 2 · 2012: 3
FWCI
1.39
Type
journal article
Language
en
Open access
Licence
journals.asm.org/non-commercial-tdm-license (tdm)
Locations
  • Infection and Immunity (publishedVersion)
  • Europe PMC (PubMed Central) (publishedVersion)
  • PubMed Central (publishedVersion)
  • PubMed (publishedVersion)
Topics
  • Life Sciences › Immunology and Microbiology › Immunology › Complement system in diseases
  • Life Sciences › Immunology and Microbiology › Immunology › Biomarkers in Disease Mechanisms
  • Life Sciences › Immunology and Microbiology › Immunology › T-cell and B-cell Immunology
Keywords
pneumococcal polysaccharide, C3d, CR2, B cell activation, complement system, alternative complement pathway, immunogenicity, T-independent antigens, antibody response regulation, C1q deficiency
MeSH terms
Adult, Antibodies, Bacterial, Antigens, Differentiation, B-Lymphocyte, B-Lymphocytes, Complement Activation, Humans, Polysaccharides, Bacterial, Receptors, Complement, Streptococcus pneumoniae, Complement C3d, Receptors, Complement 3d

About these checks

Registry, retraction, Cites and statistics results are computed, not judged, with one exception: a printed reference that no index lists is read once by a model before it counts as a citation error. Claims are extracted by a model and kept only when their quote appears verbatim in the text; each cited source is then checked, with a model’s help, for whether it says what the claim relies on. Those verdicts can be wrong — read the claim’s quote and the cited source before relying on one.

Statistics and arithmetic are recomputed, not judged.

Confidence bands are shown only for fields calibrated on our annotated test set. None is calibrated yet, so no band is shown.

What counts as a claim ›How we measure confidence ›How we check this ›

Are you an author, or do you see a problem with this page? Request a review