Immunochemical characterization of two surface polysaccharides of Bacteroides fragilis
Open accessAbstract
Immunochemical analysis of the capsular polysaccharide from Bacteroides fragilis NCTC 9343 revealed a novel structure composed of two distinct polysaccharides. Immunoelectrophoresis of an extract of purified surface polysaccharide from fermenter-grown organisms showed a complex precipitin profile with varying anodal mobility. DEAE-Sephacel anion-exchange chromatography of the polysaccharide extract failed to separate the majority of this aggregate. Disaggregation of this complex was accomplished by very mild acid treatment; purification was achieved by DEAE-Sephacel anion-exchange chromatography. Polysaccharide A had a neutral charge at pH 7.3, a net negative charge at pH 8.6, and an average Mr = 110,000; chemical analysis showed it to contain galactose, galactosamine, and an unidentified amino sugar. Polysaccharide B eluted from the anion-exchange column with increased salt concentration; it had a net negative charge and an average Mr = 200,000, and contained fucose, galactose, quinovosamine, galacturonic acid, and glucosamine. Neither of these polysaccharides contained detectable 3-deoxy-D-manno-octolusonic acid, and both were recognized as distinct antigens on the basis of their reactivity with monoclonal antibodies CE3 and F10, which reacted with the complex before acid treatment. These data indicate that the capsule of B. fragilis NCTC 9343 comprises two discrete, surface-exposed polysaccharides with differing physiochemical properties that are distinct from the lipopolysaccharide of this organism. The finding of two surface polysaccharides has not been described for other bacteria pathogenic to humans.
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PSA is produced by the common gut bacterium Bacteroides fragilis.
“Another important microbial immune modulator is the zwitterionic PSA, produced by the common gut bacterium Bacteroides fragilis (Pantosti et al., 1991; Sharma et al., 2017) (Fig. 3).”
From Understanding the Holobiont: How Microbial Metabolites Affect Human Health and Shape the Immune System (2017) · cites this paper as Pantosti et al., 1991
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- Authors
- Pantosti, A., Tzianabos, A. O., Onderdonk, A. B., & Kasper, D. L.
- Affiliations
- Brigham and Women's Hospital (US)
- Venue
- Infection and Immunity
- Publisher
- American Society for Microbiology
- Published
- 1991-06
- Volume
- 59
- Issue
- 6
- Pages
- 2075-2082
- ISSN
- 0019-9567 (ISSN-L)
- 1098-5522
- PMC
- PMC257968
- OpenAlex
- W1823444145
- Cited by
- 110
- References
- 46
- Citations by year
- 2026: 5 · 2025: 2 · 2024: 6 · 2023: 3 · 2022: 4 · 2021: 5 · 2020: 2 · 2019: 5 · 2018: 5 · 2017: 3 · 2016: 5 · 2015: 1 · 2014: 3 · 2013: 2
- FWCI
- 4.00
- Type
- journal article
- Language
- en
- Open access
- bronze
- doi.org
- Locations
- Infection and Immunity (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- National Institutes of Health (5r01ai022807-05)
- National Institute of Allergy and Infectious Diseases (1R01 AI 22807)
- Topics
- Life Sciences › Immunology and Microbiology › Microbiology › Bacterial Infections and Vaccines
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- Bacteroides fragilis, capsular polysaccharide, surface polysaccharides, polysaccharide A, polysaccharide structure, monoclonal antibodies, galacturonic acid, amino sugars, bacterial capsule
- MeSH terms
- Animals, Antibodies, Monoclonal, Bacterial Outer Membrane Proteins, Bacteroides fragilis, Carbohydrates, Chromatography, Ion Exchange, Electrophoresis, Polyacrylamide Gel, Fluorescent Antibody Technique, Hydrogen-Ion Concentration, Immunochemistry, Immunoelectrophoresis, Mice, Inbred BALB C, Molecular Weight, Polysaccharides, Bacterial, Rabbits, Mice
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