Unusual, Virulence Plasmid-Dependent Growth Behavior of Yersinia enterocolitica in Three-Dimensional Collagen Gels
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As a first approach to establishing a three-dimensional culture infection model, we studied the growth behavior of the extracellular pathogen Yersinia enterocolitica in three-dimensional collagen gels (3D-CoG). Surprisingly, we observed that plasmidless Y. enterocolitica was motile in the 3D-CoG in contrast to its growth in traditional motility agar at 37 degrees C. Motility at 37 degrees C was abrogated in the presence of the virulence plasmid pYV or the exclusive expression of the pYV-located Yersinia adhesion gene yadA. YadA-producing yersiniae formed densely packed (dp) microcolonies, whereas pYVDelta yadA-carrying yersiniae formed loosely packed microcolonies at 37 degrees C in 3D-CoG. Furthermore, we demonstrated that the packing density of the microcolonies was dependent on the head domain of YadA. Moreover, dp microcolony formation did not depend on the capacity of YadA to bind to collagen fibers, as demonstrated by the use of yersiniae producing collagen nonbinding YadA. By using a yopE-gfp reporter, we demonstrated Ca(2+)-dependent expression of this pYV-localized virulence gene by yersiniae in 3D-CoG. In conclusion, this study revealed unique plasmid-dependent growth behavior of yersiniae in a three-dimensional matrix environment that resembles the behavior of yersiniae (e.g., formation of microcolonies) in infected mouse tissue. Thus, this 3D-CoG model may be a first step to a more complex level of in vitro infection models that mimic living tissue, enabling us to study the dynamics of pathogen-host cell interactions.
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YadA is crucial for packing density of microcolonies observed in Y. enterocolitica in collagen gels.
“The Yersinia adhesin YadA is involved in autoaggregation [9] and is crucial for the packing density of microcolonies observed in Y. enterocolitica in collagen gels [145].”
From Bacterial autoaggregation (2018) · cites this paper as [145]
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- Authors
- Freund, S., Czech, B., Trülzsch, K., Ackermann, N., & Heesemann, J.
- Affiliations
- Max-von-Pettenkofer-Institut für Hygiene und Medizinische Mikrobiologie (DE)
- Max von Pettenkofer Institute for Hygiene and Medical Microbiology, Ludwig Maximilians University, D-80336 Munich, Germany
- Corresponding author
- Freund, Sandra
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 2008-06-15
- Volume
- 190
- Issue
- 12
- Pages
- 4111-4120
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC2446754
- OpenAlex
- W2109033272
- Cited by
- 31
- References
- 44
- Citations by year
- 2024: 9 · 2020: 2 · 2019: 1 · 2018: 2 · 2017: 1 · 2016: 1 · 2014: 2 · 2013: 2 · 2012: 1
- FWCI
- 1.90
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- Europe PMC (PubMed Central) (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Yersinia bacterium, plague, ectoparasites research
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Molecular Biology › Bacterial biofilms and quorum sensing
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Endocrinology › Escherichia coli research studies
- Keywords
- Yersinia enterocolitica, YadA, three-dimensional collagen gel, microcolony formation, bacterial motility, YopE, collagen binding, infection model, host-pathogen interaction
- MeSH terms
- Bacterial Outer Membrane Proteins, Bacterial Proteins, Collagen, Electrophoresis, Polyacrylamide Gel, Gels, Plasmids, Virulence, Yersinia enterocolitica, Polymerase Chain Reaction, Adhesins, Bacterial, Microscopy, Electron, Transmission
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