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Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid

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Meyers, J A, Sanchez, D, Elwell, L P, Falkow, S·1976·Journal of Bacteriology

Abstract

Agarose gel electrophoresis may be employed effectively for the detection and preliminary characterization of plasmid deoxyribonucleic acid (DNA) present in clinical isolates and laboratory strains of gram-negative microorganisms. The method is sensitive and does not require radioisotopes or ultracentrifugation. The estimation of plasmid mass from the extent of DNA migration in gels compares favorably with results obtained by electron microscopy of plasmid DNA purified by equilibrium density centrifugation. The method has proved to be a useful tool for survey work and the epidemiological investigation of plasmid dissemination, as well as an important adjunct to the genetic analysis of plasmids.

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Metadata

Authors
Meyers, J. A., Sanchez, D., Elwell, L. P., & Falkow, S.
Venue
Journal of Bacteriology
Published
1976-09
Volume
127
Issue
3
Pages
1529-1537
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
OpenAlex
W2015098348
Cited by
1,098
References
0
FWCI
38.77
Type
journal article
Language
en
Open access
bronze

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CoChat. (2026). Citation check: Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. https://app.cochat.ai/papers/10.1128/jb.127.3.1529-1537.1976
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Meyers, J. A., Sanchez, D., Elwell, L. P., & Falkow, S. (1976). Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology. https://doi.org/10.1128/jb.127.3.1529-1537.1976

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Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.