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Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid
Open accessAbstract
Agarose gel electrophoresis may be employed effectively for the detection and preliminary characterization of plasmid deoxyribonucleic acid (DNA) present in clinical isolates and laboratory strains of gram-negative microorganisms. The method is sensitive and does not require radioisotopes or ultracentrifugation. The estimation of plasmid mass from the extent of DNA migration in gels compares favorably with results obtained by electron microscopy of plasmid DNA purified by equilibrium density centrifugation. The method has proved to be a useful tool for survey work and the epidemiological investigation of plasmid dissemination, as well as an important adjunct to the genetic analysis of plasmids.
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Citations
Cited by
Illnesses Associated with Escherichia coli 0157:H7 Infections: A Broad Clinical Spectrum
Griffin, P. M., Ostroff, S. M., Tauxe, R. V., Greene, K. D., Wells, J. G., Lewis, J. H., & Blake, P. A. (1988) · Annals of Internal Medicine · doi:10.7326/0003-4819-109-9-705 · Cited by 503
Emergence of Resistance to Imipenem During Therapy for Pseudomonas aeruginosa Infections
Quinn, J. P., Dudek, E. J., DiVincenzo, C. A., Lucks, D. A., & Lerner, S. A. (1986) · Journal of Infectious Diseases · doi:10.1093/infdis/154.2.289 · Cited by 276
Friedrich, B., Hogrefe, C., & Schlegel, H. G. (1981) · Journal of Bacteriology · doi:10.1128/jb.147.1.198-205.1981 · Cited by 273
Plasmids in the Genus Bifidobacterium
Sgorbati, B., Scardovi, V., & Leblanc, D. J. (1982) · Microbiology · doi:10.1099/00221287-128-9-2121 · Cited by 76
Plasmid profiles ofMycobacterium fortuitum complex isolates
Labidi, A., Dauguet, C., Goh, K. S., & David, H. L. (1984) · Current Microbiology · doi:10.1007/bf01567167 · Cited by 63
Metadata
- Authors
- Meyers, J. A., Sanchez, D., Elwell, L. P., & Falkow, S.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1976-09
- Volume
- 127
- Issue
- 3
- Pages
- 1529-1537
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W2015098348
- Cited by
- 1,098
- References
- 0
- FWCI
- 38.77
- Type
- journal article
- Language
- en
- Open access
- bronze
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- The paper
- Meyers, J. A., Sanchez, D., Elwell, L. P., & Falkow, S. (1976). Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. Journal of Bacteriology. https://doi.org/10.1128/jb.127.3.1529-1537.1976
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Contains information from the Semantic Scholar Academic Graph Datasets, made available under the ODC Attribution License (ODC-BY 1.0).
Semantic Scholar’s data is described in: Kinney, R., et al. (2023). The Semantic Scholar Open Data Platform. arXiv:2301.10140.