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Substrate specificity and transport properties of the glycerol facilitator of Escherichia coli
Open accessAbstract
The specificity of the glycerol facilitator (glpF) of Escherichia coli was studied with an osmotic method. This transport system allowed the entry of polyols (glycerol and erythritol), pentitols, and hexitols. The analogous sugars were not transported. However, urea, glycine, and DL-glyceraldehyde could use this pathway to enter the cell. The glpF protein allowed the rapid efflux of preequilibrated xylitol. Glycerol surprisingly did not inhibit the uptake of xylitol, and xylitol only slightly reduced the uptake of glycerol. The observation and the insensitivity of the xylitol transport to low temperature suggest that the facilitator behaves as a membrane channel.
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Cited by
Arsenite transport by mammalian aquaglyceroporins AQP7 and AQP9
Liu, Z., Shen, J., Carbrey, J. M., Mukhopadhyay, R., Agre, P., & Rosen, B. P. (2002) · Proceedings of the National Academy of Sciences · doi:10.1073/pnas.092131899 · Cited by 505
The Mechanism of Proton Exclusion in the Aquaporin-1 Water Channel
de Groot, B. L., Frigato, T., Helms, V., & Grubmüller, H. (2003) · Journal of Molecular Biology · doi:10.1016/j.jmb.2003.08.003 · Cited by 285
Saier, M. H. (1994) · Microbiological Reviews · doi:10.1128/mmbr.58.1.71-93.1994 · Cited by 275
Liao, Y., Chen, J., Brandt, B. W., Zhu, Y., Li, J., van Loveren, C., & Deng, D. M. (2015) · PLOS ONE · doi:10.1371/journal.pone.0122630 · Cited by 58
Liu, K., Zhang, Z., Su, Y., Ma, X., Wang, J., Yang, Q., Nie, Q., Mo, Z., Zhou, H., Zouboulis, C. C., et al. (2026) · Journal of Nanobiotechnology · doi:10.1186/s12951-026-04291-8 · Cited by 2
Metadata
- Authors
- Heller, K. B., Lin, E. C., & Wilson, T. H.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1980-10
- Volume
- 144
- Issue
- 1
- Pages
- 274-278
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- OpenAlex
- W1818208691
- Cited by
- 331
- References
- 0
- FWCI
- 3.38
- Type
- journal article
- Language
- en
- Open access
- green
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