Effect of Growth Conditions on Production of Rhamnose-Containing Cell Wall and Capsular Polysaccharides by Strains of Lactobacillus casei subsp. rhamnosus
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Strains of Lactobacillus casei subsp. rhamnosus possessing two cell wall polysaccharides, a hexosamine-containing H-polysaccharide and a rhamnose-containing R-polysaccharide, were examined for the effect of growth conditions on the production of these two components. In strain NCTC 6375, R- and H-polysaccharides accounted for an estimated 44 and 20%, respectively, of the cell wall for organisms grown in batch culture with glucose as the carbohydrate source. Growth on fructose-containing media reduced the amount of R-polysaccharide by approximately 50% without affecting the amount of H-polysaccharide. Subculture of fructose-grown organisms in glucose restored the original proportions of the two polysaccharides. Galactose- and sucrose-grown cells behaved similarly to glucose-grown cells with respect to polysaccharide production, whereas growth in rhamnose or ribose showed values close to those for fructose-grown cells. Continuous culture of strain NCTC 6375 for more than 100 generations showed a gradual and irreversible reduction of the R-polysaccharide to less than 5% of the cell wall and an increase of the H-polysaccharide to 40% of the cell wall. Other type culture strains of L. casei subsp. rhamnosus, NCIB 7473 and ATCC 7469, behaved similarly in batch and continuous culture. In contrast, strains of L. casei subsp. rhamnosus isolated at the Institute of Dental Research showed phenotypic stability with respect to the relative proportions of R- and H-polysaccharides in both batch and continuous culture. Changes in polysaccharide composition of type culture strains were also mirrored in changes in the immunogenicity of the two components and resistance to the rate of enzymic lysis of whole organisms. For L. casei subsp. rhamnosus strain NCTC 10302 the R-polysaccharide is present entirely as capsular material. The amount of R-polysaccharide produced was also markedly dependent on the carbohydrate component of the medium in batch culture and both dilution rate and nature of the limiting carbohydrate in continuous culture, varying over a 10-fold range, whereas the cell wall H-polysaccharide remained constant.
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Extraction of EPSs into two fractions has been previously observed in lactic acid bacterial strains such as L. lactis subsp. cremoris, Lactobacillus casei subsp. rhamnosus, and L. plantarum.
“The extraction of EPSs into two fractions has been previously observed in lactic acid bacterial strains such as L. lactis subsp. cremoris, Lactobacillus casei subsp. rhamnosus, and L. plantarum [26,42,43].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [43]
Some EPSs derived from bacteria, including LAB, can act as surfactants by modifying the cell surface and reducing hydrophobicity.
“Some EPSs derived from bacteria, including LAB, can act as surfactants by modifying the cell surface and reducing hydrophobicity [43].”
From Analysis of Chemical Structure and Antibiofilm Properties of Exopolysaccharides from Lactiplantibacillus plantarum EIR/IF-1 Postbiotics (2022) · cites this paper as [43]
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The biosynthesis and functionality of the cell-wall of lactic acid bacteria.
Delcour, J., Ferain, T., Deghorain, M., Palumbo, E., & Pascal Hols, A. (1999) · PubMed · Cited by 286 · see citing works
Sengupta, R., Altermann, E., Anderson, R. C., McNabb, W. C., Moughan, P. J., & Roy, N. C. (2013) · Mediators of Inflammation · doi:10.1155/2013/237921 · Cited by 287 · see citing works
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Delcour, J., Ferain, T., Deghorain, M., Palumbo, E., & Pascal Hols, A. (1999) · Antonie van Leeuwenhoek · doi:10.1023/a:1002089722581 · Cited by 262 · see citing works
“Further complications arise, as different WPS may co-exist in the same strain, as their structure may be strain-dependent, and as their composition may also vary with the growth conditions (Wicken et al. 1983).”
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Bacterial surface polysaccharides: structure and function.
Sutherland, I. W. (1988) · International Review of Cytology · doi:10.1016/s0074-7696(08)60849-9 · Cited by 60 · see citing works
Surface and Adhesion Properties of Lactobacilli
Deepika, G., & Charalampopoulos, D. (2010) · Advances in applied microbiology · doi:10.1016/s0065-2164(10)70004-6 · Cited by 55 · see citing works
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Bergmaier, D., Champagne, C. P., & Lacroix, C. (2005) · Journal of Applied Microbiology · doi:10.1111/j.1365-2672.2004.02462.x · Cited by 51 · see citing works
“In contrast, a gradual and irreversible change in the production of a rhamnose-containing cell wall polysaccharide by different Lactobacillus casei subsp. rhamnosus strains was observed during continuous free-cell fermentations (Wicken et al. 1983).”
BackgroundOperational patterns affecting lactic acid production in ultrafiltration cell recycle bioreactor
Xavier, A. M. R. B., Gonçalves, L., Moreira, J. L., & Carrondo, M. J. T. (1995) · Biotechnology and Bioengineering · doi:10.1002/bit.260450406 · Cited by 49 · see citing works
Regulation of polysaccharide formation by Streptococcus thermophilus in batch and fed-batch cultures
Petit, C., Grill, J.-P., Maâzouzi, N., & Marczak, R. (1991) · Applied Microbiology and Biotechnology · doi:10.1007/bf00164423 · Cited by 46 · see citing works
The biosynthesis and functionality of the cell-wall of lactic acid bacteria
Delcour, J., Ferain, T., Deghorain, M., Palumbo, E., & Pascal Hols, A. (1999) · Springer eBooks · doi:10.1007/978-94-017-2027-4_7 · Cited by 46 · see citing works
Structural investigation of the capsular polysaccharide from Lactobacillus kefiranofaciens K1
Mukai, T., Toba, T., Itoh, T., & Adachi, S. (1990) · Carbohydrate Research · doi:10.1016/0008-6215(90)84039-w · Cited by 45 · see citing works
Microbial polysaccharides containing 6-deoxysugars
Graber, M., Morin, A., Duchiron, F., et al. (1988) · Enzyme and Microbial Technology · doi:10.1016/0141-0229(88)90067-1 · Cited by 40 · see citing works
Lactobacilli Are Prominent in the Initial Stages of Polymicrobial Infection of Dental Pulp
Nadkarni, M. A., Simonian, M. R., Harty, D. W. S., Zoellner, H. F., Jacques, N. A., & Hunter, N. (2010) · Journal of Clinical Microbiology · doi:10.1128/jcm.01912-09 · Cited by 36 · see citing works
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Cook, R. L., Harris, R. J., & Reid, G. (1988) · Current Microbiology · doi:10.1007/bf01573472 · Cited by 32 · see citing works
Mariya, T., Huppertz, T., Beerthuyzen, M. M., van Schalkwijk, S., Janssen, P. W. M., Wels, M., Kok, J., & Bachmann, H. (2017) · Frontiers in Microbiology · doi:10.3389/fmicb.2017.01691 · Cited by 24 · see citing works
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BackgroundPurwandari, U., & Vasiljevic, T. (2009) · International Journal of Dairy Technology · doi:10.1111/j.1471-0307.2009.00501.x · Cited by 17 · see citing works
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Halttunen, T. (2008) · UTUPub (University of Turku) · Cited by 16 · see citing works
Production and recovery of rhamnose-containing polysaccharides from acinetobacter calcoaceticus
Morin, A., Duchiron, F., & MONSAN, P. F. (1987) · Journal of Biotechnology · doi:10.1016/0168-1656(87)90060-5 · Cited by 9 · see citing works
Rijn, I. V. D. (1985) · Infection and Immunity · doi:10.1128/iai.49.3.518-522.1985 · Cited by 9 · see citing works
Bergmaier, D. (2002) · Corpus Université Laval (Université Laval) · Cited by 8 · see citing works
Nadkarni, M. A., Deshpande, N., Wilkins, M. R., & Hunter, N. (2020) · BMC Genomics · doi:10.1186/s12864-020-07062-3 · Cited by 7 · see citing works
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Diosady, L. L., & Puzanov, T. (2005) · doi:10.6703/ijase.2005.3(1).19 · Cited by 7 · see citing works
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Self-citationRowaida, K. S. K., Joseph, F. F., Ashraf, N., & Sanaa, H. O. (2007) · AFRICAN JOURNAL OF BIOTECHNOLOGY · doi:10.5897/ajb2007.000-2357 · Cited by 5 · see citing works
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BackgroundMicrobial Physiology Applied to Process Optimisation: Lactic Acid Bacteria
Bruno-Bárcena, J. M., Ragout, A. L., & Siñeriz, F. (1998) · Springer eBooks · doi:10.1007/978-94-017-0643-8_6 · Cited by 4 · see citing works
Effects of Various sugars on the Protoplast Formation of Lactobacillus
Kumode, A. (1984) · Okayama Igakkai Zasshi (Journal of Okayama Medical Association) · doi:10.4044/joma1947.96.7-8_757 · Cited by 4 · see citing works
Hamatsuka, J., Maegawa, T., Nishitani, Y., et al. (2007) · Bioscience and Microflora · doi:10.12938/bifidus.26.107 · Cited by 2 · see citing works
Tang, Y. (2014) · Työväentutkimus Vuosikirja · Cited by 2 · see citing works
Purwandari, U. (2009) · Victoria University Research Repository (Victoria University) · Cited by 1 · see citing works
FUNABASHI, M. (1984) · Okayama Igakkai Zasshi (Journal of Okayama Medical Association) · doi:10.4044/joma1947.96.7-8_745
Marini, F., Speranza, S., & Gallo, M. D. (1995) · Springer eBooks · doi:10.1007/978-3-642-79906-8_52
Nadkarni, M. A., Deshpande, N. P., Wilkins, M. R., et al. (2020) · Figshare · doi:10.6084/m9.figshare.13004164
Nadkarni, M. A., Deshpande, N. P., Wilkins, M. R., et al. (2020) · Figshare · doi:10.6084/m9.figshare.13004167
Isolation and Characterization of Two Exopolysaccharides Produced by Lactobacillus plantarum EP56
Tallon, R., Bressollier, P., & Urdaci, M. C. (2003) · Research in Microbiology · doi:10.1016/j.resmic.2003.09.006 · Cited by 260 · see citing works
- Authors
- Wicken, A. J., Ayres, A., Campbell, L. K., & Knox, K. W.
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1983-01
- Volume
- 153
- Issue
- 1
- Pages
- 84-92
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC217344
- OpenAlex
- W1586696305
- Cited by
- 38
- References
- 0
- Citations by year
- 2022: 1 · 2020: 3 · 2017: 1 · 2014: 1 · 2013: 2
- FWCI
- 4.23
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Topics
- Life Sciences › Agricultural and Biological Sciences › Food Science › Probiotics and Fermented Foods
- Life Sciences › Agricultural and Biological Sciences › Food Science › Polysaccharides Composition and Applications
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Keywords
- Lactobacillus casei subsp. rhamnosus, cell wall polysaccharides, capsular polysaccharide, growth conditions, fructose, glucose, continuous culture, batch culture, phenotypic stability, immunogenicity
- MeSH terms
- Cell Wall, Culture Media, Fructose, Glucose, Lacticaseibacillus casei, Polysaccharides, Bacterial, Rhamnose, Sucrose
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