The DOI matches its Crossref record; 50 of the 50 references it cites were found in a registry or an index.
Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production
Open accessAbstract
Summary: Exopolysaccharides contribute significantly to attachment and biofilm formation in the opportunisitc pathogen Pseudomonas aeruginosa. The Psl polysaccharide, which is synthesized by the p olysaccharide s ynthesis l ocus (psl), is required for biofilm formation in non‐mucoid strains that do not rely on alginate as the principal biofilm polysaccharide. In‐frame deletion and complementation studies of individual psl genes revealed that 11 psl genes, pslACDEFGHIJKL, are required for Psl production and surface attachment. We also present the first structural analysis of the psl‐dependent polysaccharide, which consists of a repeating pentasaccharide containing d‐mannose, d‐glucose and l‐rhamnose: image In addition, we identified the sugar nucleotide precursors involved in Psl generation and demonstrated the requirement for GDP‐d‐mannose, UDP‐d‐glucose and dTDP‐l‐rhamnose in Psl production and surface attachment. Finally, genetic analyses revealed that wbpW restored Psl production in a pslB mutant and pslB promoted A‐band LPS synthesis in a wbpW mutant, indicating functional redundancy and overlapping roles for these two enzymes. The structural and genetic data presented here provide a basis for further investigation of the Psl proteins and potential roles for Psl in the biology and pathogenesis of P. aeruginosa.
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Self-citationBiofilm formation by Pseudomonas aeruginosa wild type, flagella and type IV pili mutants
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Cited by
Flemming, H.-C., & Wingender, J. (2010) · Nature Reviews Microbiology · doi:10.1038/nrmicro2415 · Cited by 10,137
Pseudomonas aeruginosa Lifestyle: A Paradigm for Adaptation, Survival, and Persistence
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Self-citationTseng, B. S., Zhang, W., Harrison, J. J., Quach, T. P., Song, J. L., Penterman, J., Singh, P. K., Chopp, D. L., Packman, A. I., & Parsek, M. R. (2013) · Environmental Microbiology · doi:10.1111/1462-2920.12155 · Cited by 466
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Metadata
- Authors
- Byrd, M. S., Sadovskaya, I., Vinogradov, E., Lu, H., Sprinkle, A. B., Richardson, S. H., Ma, L., Ralston, B., Parsek, M. R., Anderson, E. M., Lam, J. S., & Wozniak, D. J.
- Venue
- Molecular Microbiology
- Publisher
- Wiley
- Published
- 2009-08
- Volume
- 73
- Issue
- 4
- Pages
- 622-638
- ISSN
- 0950-382X (ISSN-L)
- 1365-2958
- OpenAlex
- W1969537603
- Cited by
- 386
- References
- 50
- FWCI
- 5.52
- Type
- journal article
- Language
- en
- Open access
- bronze
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- Byrd, M. S., Sadovskaya, I., Vinogradov, E., Lu, H., Sprinkle, A. B., Richardson, S. H., Ma, L., Ralston, B., Parsek, M. R., Anderson, E. M., Lam, J. S., & Wozniak, D. J. (2009). Genetic and biochemical analyses of the Pseudomonas aeruginosa Psl exopolysaccharide reveal overlapping roles for polysaccharide synthesis enzymes in Psl and LPS production. Molecular Microbiology. https://doi.org/10.1111/j.1365-2958.2009.06795.x
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