The DOI matches its Crossref record; 22 of the 22 references it cites were found in a registry or an index.
Operator sequences of the aerobactin operon of plasmid ColV-K30 binding the ferric uptake regulation (fur) repressor
Open accessAbstract
The promoter region of the pColV-K30-encoded operon specifying biosynthesis and transport of the siderophore aerobactin was subjected to deletion analysis to determine the smallest DNA sequence affording iron regulation of a iucA'-'lacZ gene fusion. A 78-base-pair (bp) region containing the main (P1) promoter retained the character of inducibility under iron starvation. A 250-bp fragment carrying this sequence was examined for protection against DNase I by the Fur protein, the product of a gene (fur) required for negative control of several iron-regulated functions. The DNase I footprints, in the presence of various divalent heavy-metal ions added as corepressors, revealed two contiguous binding sites with different lengths and affinities for Fur. Increased concentrations of the protein appeared to elicit formation of repressor oligomers which bind to the upstream and downstream regions of the P1 promoter in a metal-dependent fashion, but with a presently undefined stoichiometry. The primary site for Fur binding spans 31 bp and contains two overlapping symmetry dyads which share the sequence 5'-TCATT-3'. It also contains extensive homology with a 19-bp consensus sequence for iron-regulated genes as deduced from comparison with the fhuA and fepA putative promoter sequences.
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“Consensus sequences bound by Fur in E. coli , P. aeruginosa , V. cholerae and C. jejuni are also AT rich 10 , 17 , 30 , 32 .”
From Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator (2015) · cites this paper as [32]
Citations
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Self-citationInteractions between DNA-bound repressors govern regulation by the λ phage repressor
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Aerobactin biosynthesis and transport genes of plasmid ColV-K30 in Escherichia coli K-12
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“an allosteric change in the protein that results in Fur binding to conserved nucleotide sequences which often overlap the promoter region of Fur-regulated genes (40, 48).”
Oliveira, F., Lima, T., Correia, A., Silva, A. M., Soares, C., Morais, S., Weißelberg, S., Vilanova, M., Rohde, H., & Cerca, N. (2022) · Microbiology Spectrum · doi:10.1128/spectrum.02168-21 · Cited by 12
“Identification of putative Fur boxes was performed with the FIMO tool (47), using the default parameters and the 19-bp Fur box consensus sequence 59-GATAATGATAATCATTATC-39 (48) as the input motif.”
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“coli is a 17 kDa polypeptide (253) which acts as a transcriptional regulator of iron-regulated promoters by virtue of its Fe-dependent DNA binding activity (13, 73).”
BackgroundCharacterization of iron uptake systems in Campylobacter jejuni
Raphael, B. H. (2002) · UA Campus Repository (The University of Arizona)
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“The promoter region of asbE was shown to contain a putative binding site for the Fur protein or Fur box with an identity of 13 out of 19 residues to the consensus Fur box sequence described in E. coli (de Lorenzo et al. 1987).”
BackgroundBollinger, N. (2001) · Montana State University ScholarWorks (Montana State University)
Drivers and Implementation of Change : An attempt to depict successful factors
Dentinger, S., & Derlyn, E. (2009) · KTH Publication Database DiVA (KTH Royal Institute of Technology)
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Metadata
- Authors
- de Lorenzo, V, Wee, S., Herrero, M., & Neilands, J. B.
- Affiliations
- University of California, Berkeley (US)
- Venue
- Journal of Bacteriology
- Publisher
- American Society for Microbiology
- Published
- 1987-06
- Volume
- 169
- Issue
- 6
- Pages
- 2624-2630
- ISSN
- 0021-9193 (ISSN-L)
- 1098-5530
- PMC
- PMC212138
- OpenAlex
- W2131282911
- Cited by
- 536
- References
- 22
- Citations by year
- 2026: 2 · 2025: 3 · 2024: 7 · 2023: 5 · 2022: 6 · 2021: 8 · 2020: 9 · 2019: 4 · 2018: 8 · 2017: 6 · 2016: 13 · 2015: 12 · 2014: 11 · 2013: 9 · 2012: 21
- FWCI
- 14.06
- Type
- journal article
- Language
- en
- Open access
- green
- www.ncbi.nlm.nih.gov
- Locations
- Journal of Bacteriology (publishedVersion)
- PubMed Central (publishedVersion)
- PubMed (publishedVersion)
- Funders
- Ministerio de Economía y Competitividad
- National Institutes of Health (1f05tw003577-01)
- U.S. Public Health Service (A104156)
- Fogarty International Center (1F05TWO3577-01-BI-5)
- Topics
- Life Sciences › Biochemistry, Genetics and Molecular Biology › Genetics › Bacterial Genetics and Biotechnology
- Life Sciences › Immunology and Microbiology › Microbiology › Escherichia coli research studies
- Health Sciences › Medicine › Hematology › Iron Metabolism and Disorders
- Keywords
- Fur repressor, iron regulation, promoter, DNase I footprinting, repressor binding sites, consensus sequence, siderophore biosynthesis, Escherichia coli
- MeSH terms
- Bacterial Proteins, Chromosome Deletion, Cloning, Molecular, Bacteriocin Plasmids, Escherichia coli, Gene Expression Regulation, Hydroxamic Acids, Iron, Operator Regions, Genetic, Operon, Promoter Regions, Genetic, Recombinant Fusion Proteins, Repressor Proteins, Transcription Factors
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