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A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope

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Vos, P, van Asseldonk, M, van Jeveren, F, Siezen, R, Simons, G, de Vos, W M·1989·Journal of Bacteriology
DOI 10.1128/jb.171.5.2795-2802.1989
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Abstract

The complete nucleotide sequence of a gene located immediately upstream of the Lactococcus lactis subsp. cremoris SK11 prtP gene encoding the cell envelope-attached proteinase was determined. This gene, designated prtM, was found to be transcribed from the same promotor region as was the proteinase gene but in the opposite direction. The prtM gene directed the expression in Escherichia coli of a protein with a size similar to the expected value of 33 kilodaltons, as deduced from the nucleotide sequence data. The derived amino acid sequence of the PrtM protein indicated the presence of a consensus lipoprotein signal sequence at the N terminus, which suggested that PrtM is a lipoprotein. Plasmids containing the prtM gene, the prtP gene, or both were constructed. Expression studies of L. lactis clones containing these plasmids showed that the prtM gene encodes a trans-acting activity involved in the maturation of cell envelope-located and -secreted forms of the SK11 proteinase.

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Metadata

Authors
Vos, P., van Asseldonk, M., van Jeveren, F., Siezen, R., Simons, G., & de Vos, W. M.
Venue
Journal of Bacteriology
Publisher
American Society for Microbiology
Published
1989-05
Volume
171
Issue
5
Pages
2795-2802
ISSN
  • 0021-9193 (ISSN-L)
  • 1098-5530
DOI
10.1128/jb.171.5.2795-2802.1989
OpenAlex
W1651369685
Cited by
168
References
1
FWCI
6.49
Type
journal article
Language
en
Open access
green
Licence
journals.asm.org/non-commercial-tdm-license (tdm)

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CoChat. (2026). Citation check: A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope. https://app.cochat.ai/papers/10.1128/jb.171.5.2795-2802.1989
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Vos, P., van Asseldonk, M., van Jeveren, F., Siezen, R., Simons, G., & de Vos, W. M. (1989). A maturation protein is essential for production of active forms of Lactococcus lactis SK11 serine proteinase located in or secreted from the cell envelope. Journal of Bacteriology. https://doi.org/10.1128/jb.171.5.2795-2802.1989

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